| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,500 | T→C | 35.2% | L375L (TTA→CTA) ‡ | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,500 | 0 | T | C | 35.2% | 51.6 / 49.5 | 54 | L375L (TTA→CTA) ‡ | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base T (12/23); new base C (12/7); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT > minE/775430‑775564 | aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACt > 1:1124496/1‑71 (MQ=255)aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACt > 1:3263982/1‑71 (MQ=255) ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa < 1:3803664/71‑1 (MQ=255) tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa < 1:3346182/69‑1 (MQ=255) ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:1377093/70‑1 (MQ=255) ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:3127539/70‑1 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGa < 1:1239885/68‑1 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGa < 1:1499814/68‑1 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg < 1:2283565/68‑1 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:3301351/1‑71 (MQ=255) cTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg > 1:1714068/1‑63 (MQ=255) tGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:3162991/1‑71 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:2997594/71‑1 (MQ=255) gacgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:2011454/68‑1 (MQ=255) ctaatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:3770846/68‑1 (MQ=255) atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:1334831/71‑1 (MQ=255) tgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:164725/70‑1 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa < 1:3803803/48‑1 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa > 1:2101086/1‑45 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:2660882/71‑2 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:1458380/1‑70 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:3095905/1‑70 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGaa < 1:236511/54‑1 (MQ=255) cgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg > 1:339422/1‑49 (MQ=37) cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg > 1:352914/1‑43 (MQ=255) gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:3032286/1‑62 (MQ=255) acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTaaa < 1:3699681/38‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:890819/1‑71 (MQ=255) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGTTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:2453484/1‑71 (MQ=255) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:841539/1‑71 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa > 1:248786/1‑55 (MQ=255) gggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct > 1:2044335/1‑69 (MQ=255) gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa > 1:2328811/1‑54 (MQ=255) gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc > 1:3033035/1‑69 (MQ=255) ggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActt < 1:1609592/70‑3 (MQ=255) ttGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATg < 1:2920408/71‑1 (MQ=255) tGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGa > 1:889719/1‑71 (MQ=255) gACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg < 1:2726767/38‑1 (MQ=255) gACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACt < 1:1054709/71‑1 (MQ=255) ctgAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc > 1:226522/3‑71 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTc < 1:3111728/71‑1 (MQ=255) cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTc > 1:1353041/1‑70 (MQ=255) cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:2441030/71‑1 (MQ=255) cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:587455/71‑1 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:1300231/42‑1 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaa > 1:583720/1‑70 (MQ=255) aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGc < 1:775697/71‑1 (MQ=255) cGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa < 1:378070/58‑1 (MQ=25) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:353651/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct < 1:819651/54‑2 (MQ=25) cTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:456989/36‑1 (MQ=255) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa > 1:318741/1‑54 (MQ=21) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc > 1:3342495/1‑61 (MQ=25) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc < 1:1910208/68‑1 (MQ=255) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgc > 1:1044885/1‑37 (MQ=255) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGAGAAAAcc > 1:1837481/1‑44 (MQ=255) | AAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT > minE/775430‑775564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |