| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,607 | C→A | 15.5% | D410E (GAC→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,607 | 0 | C | A | 15.5% | 129.1 / 8.2 | 45 | D410E (GAC→GAA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (20/18); new base A (5/2); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
AAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGT > minE/775537‑775676 | aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAc < 1:1276596/71‑1 (MQ=255)aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAc < 1:2238057/71‑1 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:540875/71‑1 (MQ=255) cGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAagcg > 1:1092867/1‑70 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcat > 1:3354160/1‑71 (MQ=255) cTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 1:1127717/65‑1 (MQ=255) tATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCg > 1:1698382/1‑70 (MQ=255) aTGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGa > 1:2398538/1‑70 (MQ=255) tGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa > 1:2320314/1‑70 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa < 1:950675/70‑1 (MQ=255) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg > 1:932792/1‑71 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAagc > 1:1887323/1‑54 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACAt < 1:3071595/69‑1 (MQ=255) tggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGt < 1:3379597/71‑1 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:1691546/1‑71 (MQ=255) tggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa > 1:788489/1‑48 (MQ=255) gcgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:2936314/1‑56 (MQ=255) aaaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:3725205/1‑70 (MQ=255) aaaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:1956097/1‑70 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta > 1:1062547/1‑71 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:3109982/47‑1 (MQ=255) aCTGCTGAAATGATCAGAGTTTGGTGACGCGATCATCGaaa > 1:1191619/1‑41 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:1931516/67‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttat > 1:3094396/1‑70 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGAAGCGatcatc > 1:908422/1‑36 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGAAGCGatcatc > 1:78610/1‑36 (MQ=255) gctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg > 1:3504427/1‑58 (MQ=255) gctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg > 1:874273/1‑58 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatta < 1:3105785/69‑1 (MQ=255) gAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:3643202/47‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:367322/1‑35 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACggg < 1:1370934/71‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACgg < 1:2763833/70‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg < 1:3778791/39‑1 (MQ=255) aaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa < 1:177902/71‑1 (MQ=255) aTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:823773/1‑37 (MQ=255) aTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt > 1:194820/1‑38 (MQ=255) aTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAg > 1:3261257/1‑71 (MQ=255) ttCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatta < 1:3182803/61‑1 (MQ=255) ttCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatta < 1:1991316/61‑1 (MQ=255) cAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:1767977/1‑54 (MQ=255) gagTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAttt > 1:1912298/1‑53 (MQ=255) agTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:2393484/1‑51 (MQ=255) agTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGc > 1:3900617/1‑71 (MQ=255) tttGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:1739446/49‑1 (MQ=255) tttGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:2623190/49‑1 (MQ=255) tttGGTGACGCGATAATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGt < 1:712975/64‑1 (MQ=255) gTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACgg < 1:115158/54‑1 (MQ=255) tGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg < 1:1420201/37‑1 (MQ=255) aCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgt < 1:458473/71‑1 (MQ=255) cgcgATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTaa > 1:412275/1‑47 (MQ=255) | AAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGT > minE/775537‑775676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |