| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 788,075 | G→A | 28.6% | P461S (CCA→TCA) | ycgB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 788,075 | 0 | G | A | 28.6% | 61.0 / 26.9 | 42 | P461S (CCA→TCA) | ycgB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/18); new base A (6/6); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCA > minE/788009‑788143 | cccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAat < 1:548767/70‑1 (MQ=255) ccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAatat < 1:187626/71‑1 (MQ=255) gCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCa < 1:673941/71‑1 (MQ=255) tGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGAGt < 1:1441794/71‑1 (MQ=255) acaTGCTTCAGGACTTCTTTGCGCCCCCGATCTAGCGGTGCGCGATTATGTgg < 1:3431034/53‑1 (MQ=255) aTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCg < 1:1584187/71‑1 (MQ=255) tGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGa < 1:3335757/71‑1 (MQ=255) cTTCAGTACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGaa < 1:533717/70‑1 (MQ=255) cttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGt < 1:1123299/66‑1 (MQ=255) cttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTcgccg > 1:156816/1‑71 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTcgccgc > 1:1106077/1‑71 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTcgccgc > 1:2192647/1‑71 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTcgccgc > 1:1950734/1‑71 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTcgccg > 1:2175364/1‑70 (MQ=255) cttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCcgcg > 1:230876/1‑70 (MQ=255) cttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCcgcg > 1:2605247/1‑70 (MQ=255) tGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCaa < 1:854101/70‑1 (MQ=255) gcgcCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTc > 1:2591487/1‑49 (MQ=255) gcgcCCCCGATCCAGCGGTGAGCGATTATGTGGAATATAACGCAGCGTc > 1:1508299/1‑49 (MQ=255) gcCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATcc < 1:1182818/71‑1 (MQ=255) cccTATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGt < 1:2856702/54‑1 (MQ=255) cccGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCAc > 1:3502551/1‑70 (MQ=255) cGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGtt > 1:3847548/1‑71 (MQ=255) gATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCa < 1:1748347/60‑1 (MQ=255) aTCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGt < 1:3092849/50‑1 (MQ=255) aTCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTcc < 1:2894773/71‑1 (MQ=255) tCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTcgccgc < 1:2031779/55‑1 (MQ=255) tCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTcc < 1:2979154/70‑1 (MQ=255) cAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAAc > 1:3235093/1‑44 (MQ=255) cAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAg < 1:2354668/70‑1 (MQ=255) aGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTc > 1:340937/1‑66 (MQ=255) cGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCt < 1:3056880/71‑1 (MQ=255) ggggcgcGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCt > 1:1141065/4‑70 (MQ=255) ggTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTg < 1:2609896/71‑1 (MQ=255) gTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTg > 1:186860/1‑70 (MQ=255) cgcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTc > 1:830455/1‑40 (MQ=255) cgcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAata < 1:926751/71‑1 (MQ=255) cgcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAata < 1:3437630/71‑1 (MQ=255) cgcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGACCTGAata < 1:3291789/71‑1 (MQ=255) gcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAatat > 1:3238249/1‑71 (MQ=255) ttATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCg > 1:3184303/1‑70 (MQ=255) gtgGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTc < 1:3719746/70‑1 (MQ=255) tgGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCa < 1:3811341/71‑1 (MQ=255) ggAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTc > 1:1054525/1‑68 (MQ=255) | CCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCA > minE/788009‑788143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |