Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	806,555	C→T	12.7%	G538S (GGC→AGC)	ycgV ←	predicted adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	806,555	0	C	T	12.7%	144.7 / 10.7	55	G538S (GGC→AGC)	ycgV	predicted adhesin
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (5/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

GATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGT  >  minE/806489‑806624                                                                   |                                                                      gATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                    >  1:3742511/1‑70 (MQ=255) gATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCca                                                                      >  1:592351/1‑68 (MQ=255)  aTTACCAGCACTACTCCCGCTTATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCtaaca                                                                   <  1:3226800/70‑1 (MQ=255)   ttACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                                 <  1:2425884/71‑1 (MQ=255)      ccAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                               <  1:153728/70‑1 (MQ=255)       cAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                               <  1:3647084/69‑1 (MQ=255)       cAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt                                                               >  1:2590970/1‑69 (MQ=255)         gCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAg                                                            >  1:2264446/1‑70 (MQ=255)           actactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaaca                                                                   >  1:1301087/1‑61 (MQ=255)            ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACg                                                        >  1:1761830/1‑71 (MQ=255)             tactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCa                                                          <  1:3906033/68‑1 (MQ=255)             tactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCa                                                          <  1:2590908/68‑1 (MQ=255)                  ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                       >  1:878243/1‑50 (MQ=255)                  ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                       >  1:1499559/1‑50 (MQ=255)                    gCTGATATTCAATAAATCCCCTTTATTATTACCGCCATTACCCTCGCCAACAACATCAGCACGCataata                                                 >  1:3362487/1‑70 (MQ=255)                    gCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataat                                                  >  1:664637/1‑69 (MQ=255)                     cTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                               <  1:1277313/71‑1 (MQ=255)                       gATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                       <  1:1122619/45‑1 (MQ=255)                        atatTCAATAAATCCCGTTTATTATTAACGCCATTACCCTCGcc                                                                       <  1:3329623/44‑1 (MQ=255)                         tatTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                           <  1:1345904/71‑1 (MQ=255)                          atTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                           <  1:857157/70‑1 (MQ=255)                           ttCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAg                                              >  1:2724006/1‑66 (MQ=255)                             cAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTa                                           >  1:805735/1‑67 (MQ=255)                              aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGt                                       <  1:208115/70‑1 (MQ=255)                                tAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTa                                     >  1:1267776/1‑70 (MQ=255)                                tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc                                    >  1:830969/1‑71 (MQ=255)                                  aaTCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                                  >  1:3653407/1‑39 (MQ=255)                                  aaTCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                                  >  1:2901106/1‑39 (MQ=255)                                   aTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc                                 >  1:1444327/1‑71 (MQ=255)                                   aTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc                                 <  1:2422555/71‑1 (MQ=255)                                    tCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa                                                          >  1:113345/1‑45 (MQ=255)                                     ccccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa                                     >  1:623839/1‑65 (MQ=255)                                       ccTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAg                             >  1:486504/1‑71 (MQ=255)                                        cTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt                                      <  1:874592/61‑1 (MQ=255)                                        cTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg                            >  1:3183724/1‑71 (MQ=255)                                         tttATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGt                           >  1:3175410/1‑71 (MQ=255)                                           tattattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg                                             <  1:3573125/51‑1 (MQ=255)                                            attattaACGCCATTACCCTCGCCAACAACATCAg                                                            <  1:3046883/35‑1 (MQ=255)                                            attattaACGCCATTACCCTCGCCAACAACATCAg                                                            <  1:455151/35‑1 (MQ=255)                                            attattaACGCCATTACCCTCGCCAACAACATCAg                                                            <  1:2194522/35‑1 (MQ=255)                                            attattaACGCCATTACCCTCGCCAACAACATCAg                                                            <  1:390338/35‑1 (MQ=255)                                            attattaACGCCATTACCCTCGCCAACAACATCAg                                                            <  1:1661097/35‑1 (MQ=255)                                             ttattaACGCCATTACCCTCGCCAACAACATCAGCACg                                                        >  1:2419589/1‑38 (MQ=255)                                             ttattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                       >  1:2888017/1‑71 (MQ=255)                                             ttattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                       <  1:36137/71‑1 (MQ=255)                                             ttattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc                       <  1:115249/71‑1 (MQ=255)                                                   aCGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGt                  <  1:3946843/70‑1 (MQ=255)                                                     gCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc                                    >  1:3562492/1‑50 (MQ=255)                                                     gCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt                 <  1:941432/69‑1 (MQ=255)                                                     gCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa               <  1:1270339/71‑1 (MQ=255)                                                      ccATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa              >  1:1465354/1‑71 (MQ=255)                                                       cATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCt                                >  1:2943789/1‑52 (MQ=255)                                                       cATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCt                                >  1:2939140/1‑52 (MQ=255)                                                       cATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt             <  1:397923/71‑1 (MQ=255)                                                        aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg            >  1:3641431/1‑71 (MQ=255)                                                        aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt             <  1:1418865/70‑1 (MQ=255)                                                         ttACCCTCGCCAACAACATCAGGACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg            <  1:1314586/70‑1 (MQ=255)                                                         ttACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc                                 >  1:606018/1‑49 (MQ=255)                                                         ttACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgt           >  1:599459/1‑71 (MQ=255)                                                         ttACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgt           >  1:1746192/1‑71 (MQ=255)                                                          tACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg          >  1:3507851/1‑71 (MQ=255)                                                           aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg         >  1:1108804/1‑71 (MQ=255)                                                            cccTCGCCAACAACATCAGCACGCATAATAAAGGTACt                                         >  1:3039860/1‑38 (MQ=255)                                                                cGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaaa     >  1:1935761/1‑70 (MQ=255)                                                                 gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAt    <  1:2699001/70‑1 (MQ=255)                                                                   caacaaCATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAtgt  <  1:2852140/70‑1 (MQ=255)                                                                   |                                                                      GATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGT  >  minE/806489‑806624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑