| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,576 | T→G | 41.9% | intergenic (‑113/+341) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,576 | 0 | T | G | 41.9% | 26.3 / 56.8 | 43 | intergenic (‑113/+341) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (12/13); new base G (7/11); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAA > minE/821508‑821643 | tGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTa > 1:1253158/1‑71 (MQ=11)tGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa < 1:196800/71‑1 (MQ=34)tGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:2540806/1‑71 (MQ=34) ttttGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCaa > 1:2809023/1‑71 (MQ=34) tttGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAAc > 1:2311261/1‑71 (MQ=34) gCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGt < 1:869883/70‑1 (MQ=11) gCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCgg < 1:2183978/71‑1 (MQ=11) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg < 1:770188/71‑1 (MQ=34) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg < 1:3169187/71‑1 (MQ=34) cTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCggg > 1:3039768/1‑71 (MQ=11) cTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCggg > 1:3641791/1‑71 (MQ=11) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:626545/1‑56 (MQ=35) tATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACctct > 1:135042/1‑59 (MQ=11) tATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:3402600/1‑55 (MQ=35) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGc < 1:318933/65‑1 (MQ=11) ttCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGAGGGt > 1:3699716/1‑71 (MQ=21) cTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGttt > 1:808936/1‑71 (MQ=34) ttGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAAc < 1:506088/58‑1 (MQ=11) ttGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGttt > 1:110065/1‑70 (MQ=11) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:3690061/48‑1 (MQ=38) tGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtc > 1:209765/1‑71 (MQ=34) aaGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtctc < 1:2216441/71‑1 (MQ=34) aGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt > 1:1725563/1‑43 (MQ=38) tCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTc < 1:962963/69‑1 (MQ=11) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc < 1:1157142/43‑1 (MQ=38) gTTTCAAGATTAGACCCCGTTCTGTTGTCAGGTTTTACCTCTCaa > 1:3900291/1‑45 (MQ=255) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:2743903/70‑1 (MQ=35) aaGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCa < 1:1599961/70‑1 (MQ=35) aaGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCa < 1:675432/70‑1 (MQ=35) aGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAg > 1:3529007/1‑63 (MQ=25) aGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAAt < 1:2480140/71‑1 (MQ=21) tAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:380991/1‑71 (MQ=21) aGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:1712516/1‑70 (MQ=35) cccccGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:3316450/61‑1 (MQ=35) cccccGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:877587/61‑1 (MQ=35) cccccGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccacca < 1:1957681/71‑1 (MQ=34) ccccGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTtct < 1:2517534/47‑1 (MQ=255) ccccGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAg < 1:2118913/71‑1 (MQ=21) cccGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:2147854/59‑1 (MQ=255) gTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGa < 1:3247547/70‑1 (MQ=35) ttCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATa < 1:1500648/71‑1 (MQ=34) ttCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAGTGCCACCAGGGATa < 1:2770583/71‑1 (MQ=255) tCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAggg < 1:2471799/67‑1 (MQ=18) aGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCg > 1:246674/1‑70 (MQ=33) gTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa > 1:3363216/1‑71 (MQ=38) tttACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGccccc > 1:594259/1‑65 (MQ=31) | TGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAA > minE/821508‑821643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |