| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,688 | T→A | 10.1% | intergenic (‑225/+229) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,688 | 0 | T | A | 10.1% | 158.0 / 8.3 | 59 | intergenic (‑225/+229) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (34/19); new base A (3/3); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGT > minE/821620‑821758 | ccaccaGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGtc < 1:2098884/70‑1 (MQ=255) ccaGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCaa > 1:2651770/1‑71 (MQ=255) ccaGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCaa > 1:766583/1‑71 (MQ=255) caGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAAt < 1:219669/71‑1 (MQ=255) caGGGATAAAGCCCCCGCAACATTGCGCCTAACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAAt > 1:3360830/1‑71 (MQ=11) aTAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATc > 1:3365683/1‑71 (MQ=11) aTAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATc > 1:2131324/1‑71 (MQ=11) aaGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATctt > 1:735884/1‑70 (MQ=255) ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcacttca < 1:3508590/71‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAATTCCGGCTTTGTGTGGATACTACGTCTCAATTCATcttcac > 1:5191/1‑65 (MQ=255) cAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATcc > 1:8343/1‑71 (MQ=255) cATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCt > 1:548841/1‑70 (MQ=255) ctccTCACCGGATAACTCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAAt < 1:3814752/68‑1 (MQ=11) ccTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAAt < 1:443702/44‑1 (MQ=255) cTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:1125969/1‑71 (MQ=255) cTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:2547852/71‑1 (MQ=255) cACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:1923557/1‑70 (MQ=255) cACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:3850212/1‑70 (MQ=255) aCCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:3418216/1‑70 (MQ=255) aCCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:132844/1‑70 (MQ=255) ccGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGa < 1:2700037/60‑1 (MQ=255) ccGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa < 1:587324/71‑1 (MQ=255) ccGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTAATCCCTGAAATGTTTGCa > 1:2164242/1‑70 (MQ=255) cGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATctt < 1:2581294/45‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAAt < 1:2977120/36‑1 (MQ=255) aTAATTCCGGCTTGGTGTGCATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGtt < 1:986188/62‑1 (MQ=255) aaTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:663596/1‑62 (MQ=255) aaTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:2637212/1‑62 (MQ=255) aaTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:95301/1‑62 (MQ=255) tCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAAt > 1:2033020/1‑54 (MQ=255) tCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAAt < 1:1300771/54‑1 (MQ=255) ccGGCTTGGTGTGGATACTACGTCTCAATTCATcttcac > 1:2129050/1‑39 (MQ=40) ccGGCTTGGTGTGGATACTACGACTCAATTCATcttcacttca < 1:738422/43‑1 (MQ=255) ccGGCTTGGTGTGGATACTACGACTCAATTCATcttcacttca < 1:3792300/43‑1 (MQ=255) cGGCTTGGTGTGGATACTACGTCTCAATTCATctt > 1:1488630/1‑35 (MQ=40) cGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGt > 1:596032/1‑54 (MQ=35) cGGCTTGGTGTGGATACTACGTCT‑AATTCATCTTCACTTCATCCCTGAAATGt > 1:1887740/1‑53 (MQ=11) ggCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:3193887/1‑56 (MQ=35) ggCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACAt < 1:3693528/71‑1 (MQ=39) gCTTGGTCTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGaa > 1:1170739/1‑48 (MQ=255) tGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCg > 1:300655/1‑71 (MQ=39) gtgtGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCgg < 1:1936849/70‑1 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:509117/1‑70 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:804117/1‑70 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCttt > 1:1489830/1‑69 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCttt > 1:87792/1‑69 (MQ=40) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtt < 1:198576/66‑1 (MQ=40) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa < 1:2913701/71‑1 (MQ=39) ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:66514/1‑66 (MQ=40) tacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:3239704/41‑1 (MQ=38) acGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:2209650/1‑38 (MQ=40) acGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:915968/1‑38 (MQ=40) acGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGt > 1:254168/1‑50 (MQ=255) acGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGt > 1:1683290/1‑50 (MQ=255) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAc > 1:2593556/1‑67 (MQ=40) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAg < 1:2751842/69‑1 (MQ=40) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc < 1:586455/70‑1 (MQ=40) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCt > 1:3906341/1‑71 (MQ=39) gTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTg < 1:2767886/71‑1 (MQ=39) gTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTg < 1:3762849/71‑1 (MQ=39) tctcAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGt > 1:1724755/1‑71 (MQ=39) | CCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGT > minE/821620‑821758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |