| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,760 | G→A | 37.5% | intergenic (‑297/+157) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,760 | 0 | G | A | 37.5% | 75.8 / 62.9 | 64 | intergenic (‑297/+157) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base G (16/24); new base A (12/12); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
AATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAG > minE/821692‑821825 | aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCa < 1:914898/71‑1 (MQ=34) aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCg < 1:743751/70‑1 (MQ=39) ttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg < 1:1271260/59‑1 (MQ=35) tcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg < 1:2102745/58‑1 (MQ=35) tcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg < 1:1585193/58‑1 (MQ=35) aTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGc < 1:2768597/71‑1 (MQ=14) tCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGct < 1:1387584/71‑1 (MQ=14) ccTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:1129744/62‑1 (MQ=11) cTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:2463129/71‑1 (MQ=17) cTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTg < 1:3720935/71‑1 (MQ=255) tGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAg > 1:583189/1‑63 (MQ=14) tGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTg > 1:3138346/1‑70 (MQ=255) gAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:3123666/1‑71 (MQ=17) aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat > 1:3682894/1‑70 (MQ=17) aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat > 1:240717/1‑70 (MQ=17) aaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:23727/1‑65 (MQ=255) aaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatg < 1:3007666/71‑1 (MQ=255) aTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatga < 1:1700420/71‑1 (MQ=255) aTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatga < 1:1275452/71‑1 (MQ=255) tGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag < 1:3071787/71‑2 (MQ=18) gCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:2229396/70‑1 (MQ=255) cAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct < 1:1323280/56‑1 (MQ=255) cAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct < 1:3878576/56‑1 (MQ=255) cAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTc < 1:57373/70‑1 (MQ=255) tgaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg < 1:3052251/58‑1 (MQ=21) tAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGc > 1:2248697/1‑71 (MQ=255) gaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTc > 1:1806389/2‑66 (MQ=21) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatga > 1:1164136/1‑59 (MQ=21) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:2556154/1‑56 (MQ=21) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:2005732/1‑56 (MQ=21) aaGAGTGCATTCCGGTATTTCAACAGCTGTTACAGTCATTTCATGAGAGCTCTGgatga > 1:2361553/1‑59 (MQ=21) aaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:3570292/1‑40 (MQ=40) agTGCATTCCGGTTTTTCAACAGCTGTTACAGTCAttt < 1:540275/38‑1 (MQ=11) gTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:3690362/60‑1 (MQ=255) cATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCGAGCTCggg > 1:161017/1‑67 (MQ=255) aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:661338/1‑55 (MQ=25) aTTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCt < 1:2248109/55‑1 (MQ=255) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:3387662/35‑1 (MQ=17) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:410323/35‑1 (MQ=17) tCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa > 1:1973720/1‑71 (MQ=38) tCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa > 1:2617331/1‑71 (MQ=38) tCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa > 1:2305213/1‑70 (MQ=255) cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct > 1:2129108/1‑39 (MQ=255) cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct > 1:2953427/1‑39 (MQ=255) ggCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCt > 1:2577897/1‑57 (MQ=255) gtttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt < 1:1741522/69‑1 (MQ=255) gtttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt < 1:3696396/69‑1 (MQ=255) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAttt < 1:3726787/70‑1 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat > 1:3242477/1‑42 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatga < 1:1992919/43‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt < 1:3699251/60‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAat < 1:1490457/66‑1 (MQ=255) tttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt > 1:3897959/1‑59 (MQ=255) tttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt > 1:546941/1‑59 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTc > 1:345701/1‑47 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:2325240/70‑1 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:1920230/70‑1 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACt > 1:3843171/1‑71 (MQ=255) cAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:1277908/70‑1 (MQ=255) cAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:2223815/70‑1 (MQ=255) aGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAg > 1:3075574/1‑44 (MQ=255) aGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg > 1:847166/1‑70 (MQ=255) cTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAag < 1:1648264/71‑1 (MQ=255) tGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg < 1:1008128/64‑1 (MQ=255) | AATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAG > minE/821692‑821825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |