| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,788 | T→G | 37.8% | intergenic (‑325/+129) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,788 | 0 | T | G | 37.8% | 16.4 / 62.2 | 53 | intergenic (‑325/+129) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (16/17); new base G (10/10); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > minE/821723‑821854 | gCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:2229396/70‑1 (MQ=255) cAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTc < 1:57373/70‑1 (MQ=255) tAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGc > 1:2248697/1‑71 (MQ=255) gaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTc > 1:1806389/2‑66 (MQ=21) gTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:3690362/60‑1 (MQ=255) cATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCGAGCTCggg > 1:161017/1‑67 (MQ=255) aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:661338/1‑55 (MQ=25) aTTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCt < 1:2248109/55‑1 (MQ=255) tCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa > 1:2617331/1‑71 (MQ=38) tCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa > 1:1973720/1‑71 (MQ=38) tCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa > 1:2305213/1‑70 (MQ=255) ggCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCt > 1:2577897/1‑57 (MQ=255) gtttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt < 1:1741522/69‑1 (MQ=255) gtttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt < 1:3696396/69‑1 (MQ=255) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAttt < 1:3726787/70‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt < 1:3699251/60‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAat < 1:1490457/66‑1 (MQ=255) tttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt > 1:3897959/1‑59 (MQ=255) tttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGtt > 1:546941/1‑59 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTc > 1:345701/1‑47 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:1920230/70‑1 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:2325240/70‑1 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACt > 1:3843171/1‑71 (MQ=255) cAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:2223815/70‑1 (MQ=255) cAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:1277908/70‑1 (MQ=255) aGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAg > 1:3075574/1‑44 (MQ=255) aGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg > 1:847166/1‑70 (MQ=255) cTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAag < 1:1648264/71‑1 (MQ=255) tGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg < 1:1008128/64‑1 (MQ=255) acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGAt < 1:2122423/69‑1 (MQ=255) aGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:105634/71‑1 (MQ=255) aGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAttt < 1:29036/53‑1 (MQ=255) cGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg > 1:119851/1‑37 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAat > 1:3023663/1‑45 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:1774006/1‑70 (MQ=255) tttCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTtgt < 1:1373198/54‑1 (MQ=255) tttCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaa < 1:3172004/71‑1 (MQ=255) ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:773758/1‑65 (MQ=255) tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAtt > 1:403520/1‑45 (MQ=255) tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa > 1:278430/1‑70 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:3384180/1‑48 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:10341/1‑48 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg > 1:3869755/1‑54 (MQ=255) aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAAt < 1:3394965/71‑1 (MQ=255) tGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:662431/71‑1 (MQ=255) gAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:2399610/70‑1 (MQ=255) gTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:1656257/1‑50 (MQ=255) gTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGcc < 1:3825580/71‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGc < 1:1552748/70‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2069879/71‑1 (MQ=255) ctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1268934/69‑1 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATaaa > 1:3393867/1‑53 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCg > 1:807849/1‑67 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:668563/1‑71 (MQ=255) atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt > 1:535585/1‑71 (MQ=255) atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:2425254/71‑1 (MQ=255) tgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt > 1:1029920/1‑70 (MQ=255) atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa < 1:3756016/38‑1 (MQ=255) atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:2162208/66‑1 (MQ=255) | GCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > minE/821723‑821854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |