| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,826 | A→T | 38.6% | intergenic (‑363/+91) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,826 | 0 | A | T | 38.6% | 32.5 / 48.2 | 44 | intergenic (‑363/+91) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (9/18); new base T (7/10); total (16/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCC > minE/821760‑821887 | acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGAt < 1:2122423/69‑1 (MQ=255) aGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:105634/71‑1 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:1774006/1‑70 (MQ=255) tttCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaa < 1:3172004/71‑1 (MQ=255) ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:773758/1‑65 (MQ=255) tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa > 1:278430/1‑70 (MQ=255) aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAAt < 1:3394965/71‑1 (MQ=255) tGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:662431/71‑1 (MQ=255) gAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:2399610/70‑1 (MQ=255) gTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGcc < 1:3825580/71‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGc < 1:1552748/70‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2069879/71‑1 (MQ=255) ctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1268934/69‑1 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATaaa > 1:3393867/1‑53 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCg > 1:807849/1‑67 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:668563/1‑71 (MQ=255) atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:2425254/71‑1 (MQ=255) atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt > 1:535585/1‑71 (MQ=255) tgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt > 1:1029920/1‑70 (MQ=255) atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:2162208/66‑1 (MQ=255) gCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2080475/60‑1 (MQ=255) ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaaaa < 1:3702351/48‑1 (MQ=255) ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc > 1:3201175/1‑71 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg < 1:209352/71‑1 (MQ=255) gCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCgg > 1:55312/1‑70 (MQ=255) cTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg < 1:3689502/68‑1 (MQ=255) cGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGAc < 1:3634067/37‑1 (MQ=255) gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCg < 1:3682159/47‑1 (MQ=255) gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCg < 1:1124784/47‑1 (MQ=255) gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAAt < 1:2931438/40‑1 (MQ=255) gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAAt < 1:2992327/40‑1 (MQ=255) ttGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc < 1:1627388/71‑1 (MQ=255) ctGCCAATATTTACTTGTGGAAGTGATAAAGACaaaa < 1:1712164/36‑1 (MQ=39) gCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc > 1:2977712/1‑40 (MQ=255) cAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgc > 1:3890915/1‑71 (MQ=255) cAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:2076553/48‑1 (MQ=255) atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:3503344/40‑1 (MQ=255) atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg > 1:2478154/1‑70 (MQ=255) tatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc > 1:165946/1‑42 (MQ=255) atTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa > 1:199331/1‑70 (MQ=255) aCTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTg > 1:2829459/1‑71 (MQ=255) gtgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGcc < 1:864509/69‑1 (MQ=255) tgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTAc < 1:535924/38‑1 (MQ=255) aaGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGcc < 1:29224/45‑1 (MQ=255) agaTAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc > 1:848335/1‑38 (MQ=255) | GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCC > minE/821760‑821887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |