| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,222 | T→A | 11.7% | intergenic (‑198/+206) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,222 | 0 | T | A | 11.7% | 205.2 / 17.8 | 77 | intergenic (‑198/+206) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (40/28); new base A (2/7); total (42/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
ATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGT > minE/822152‑822292 | aTGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt < 1:1786588/71‑1 (MQ=255) accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa < 1:1319901/71‑1 (MQ=255) accaCGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa < 1:960485/71‑1 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt > 1:1118681/1‑71 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt > 1:1890918/1‑71 (MQ=255) caGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt < 1:2079661/64‑1 (MQ=255) caGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt < 1:521496/64‑1 (MQ=255) gggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:3710635/1‑71 (MQ=255) ggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCAt > 1:488825/1‑71 (MQ=255) gATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCg < 1:147665/62‑1 (MQ=255) agcccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttca < 1:1171231/68‑1 (MQ=1) aGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcac > 1:3693127/1‑71 (MQ=255) gCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTAATcttcact > 1:797305/1‑71 (MQ=255) cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCAt < 1:1966961/71‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCAGCTTCACTTCATc > 1:1616908/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc < 1:2177149/71‑1 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc > 1:1440127/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc > 1:3526780/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc > 1:3464517/1‑71 (MQ=255) cAACATTGAGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc > 1:2815525/1‑71 (MQ=255) cATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATctt < 1:3083292/58‑1 (MQ=255) cATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCt > 1:785521/1‑70 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAAt < 1:1798398/70‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATg < 1:3244186/71‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATg < 1:1434139/71‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATg < 1:1359373/71‑1 (MQ=255) ccTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:426196/1‑71 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATCttt < 1:3455081/71‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGt < 1:2084305/36‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:451728/44‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGtttt > 1:1613180/1‑70 (MQ=255) cACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:3678782/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc < 1:896309/45‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:1943507/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTAAGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:3512277/1‑70 (MQ=255) ccGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:1194245/1‑42 (MQ=255) ccGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaaa > 1:2671251/1‑62 (MQ=255) ccGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:1976913/69‑1 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:3217359/63‑1 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:3658768/1‑70 (MQ=255) ggATAACGCCGGCTTGGTGTGGATACTACGTCGCAAtt < 1:1593523/38‑1 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGACTCAAt < 1:551162/36‑1 (MQ=14) aTAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg > 1:2185348/1‑69 (MQ=255) aTAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg > 1:834305/1‑69 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg > 1:1250754/1‑68 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:2056928/1‑71 (MQ=255) cGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCATGAAATGTTTGCAATGAagag > 1:1787643/1‑70 (MQ=255) cGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:3588394/1‑70 (MQ=255) ccGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:151007/1‑56 (MQ=35) cGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:1808010/1‑71 (MQ=39) ggCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACAt < 1:168253/71‑1 (MQ=39) cTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa < 1:1692959/56‑1 (MQ=35) cTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc < 1:1373289/71‑1 (MQ=39) ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTcc > 1:819932/1‑71 (MQ=39) ggTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTcc < 1:458069/69‑1 (MQ=40) ggTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:2280375/1‑71 (MQ=39) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGc < 1:1834663/71‑1 (MQ=39) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaa < 1:2664049/40‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaa < 1:2365845/40‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGt < 1:1337933/58‑1 (MQ=255) ggATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:2626367/47‑1 (MQ=38) taTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt < 1:1831327/69‑1 (MQ=255) gATACTACGTCGCAATTCATCTTCACTTCATCCCt < 1:3536035/35‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2201998/71‑1 (MQ=39) aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCa < 1:3503504/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa < 1:582289/71‑1 (MQ=39) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa < 1:3813511/71‑1 (MQ=39) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:1901168/1‑41 (MQ=38) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:644106/1‑41 (MQ=38) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:3789318/1‑41 (MQ=38) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:2587123/1‑41 (MQ=38) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCttt > 1:3703888/1‑63 (MQ=40) acGTCGCAATTCATCTTAACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCttt > 1:153315/1‑63 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCGTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt > 1:3787728/1‑71 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:2636052/1‑58 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:366524/1‑58 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa > 1:2580893/1‑68 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa > 1:184691/1‑68 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg > 1:1772258/1‑69 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt < 1:3704765/71‑1 (MQ=39) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt < 1:3229484/71‑1 (MQ=39) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTg < 1:3484285/70‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGt < 1:1046257/71‑1 (MQ=39) | ATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGT > minE/822152‑822292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |