| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,496 | T→C | 14.1% | S331S (TCT→TCC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,496 | 0 | T | C | 14.1% | 174.7 / 18.4 | 71 | S331S (TCT→TCC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (32/29); new base C (5/5); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGC > minE/189430‑189560 | tgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCt < 1:2713246/71‑1 (MQ=255) gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg < 1:168469/71‑1 (MQ=255) gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTg < 1:2679645/70‑1 (MQ=255) cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTggg < 1:3873571/71‑1 (MQ=255) cTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTcgg < 1:2089225/71‑1 (MQ=255) tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg > 1:3499844/1‑71 (MQ=255) gCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGc > 1:87838/1‑71 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt < 1:3242697/70‑3 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa < 1:3480662/71‑1 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa > 1:3923733/1‑71 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa < 1:3537515/71‑1 (MQ=255) tACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTa > 1:2404752/1‑71 (MQ=255) tACAACACCGACTGGATCAAACAGACTCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1280254/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1474286/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg < 1:1557950/69‑1 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:540775/1‑71 (MQ=255) tACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:476107/1‑71 (MQ=255) acaacaCCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGcctt < 1:673216/69‑3 (MQ=255) acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:2144761/1‑70 (MQ=255) acaacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa > 1:1123100/1‑70 (MQ=255) caacaCCGACTTCATCAACAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTaca > 1:3742465/1‑71 (MQ=255) acagACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa > 1:669736/4‑71 (MQ=255) acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCa > 1:3811930/1‑71 (MQ=255) acCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACATAGATTCt > 1:3890559/1‑51 (MQ=255) ccGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTa < 1:59962/64‑1 (MQ=255) gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑ttt < 1:3002159/71‑1 (MQ=255) gACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑ttt < 1:757113/71‑1 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑tttt > 1:2482060/1‑71 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑tttt > 1:3379048/1‑71 (MQ=255) cTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑tttt < 1:3861866/70‑1 (MQ=255) tGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca < 1:1512994/61‑1 (MQ=255) ggATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt > 1:781594/1‑52 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACAcc > 1:755731/1‑61 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCa < 1:2709764/69‑1 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCa < 1:1265227/69‑1 (MQ=255) gATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCAt < 1:1294762/70‑1 (MQ=255) cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:1569762/1‑56 (MQ=255) cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:416130/1‑56 (MQ=255) aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATc > 1:382467/1‑71 (MQ=255) aaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATc < 1:2252234/71‑1 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTGACTTCGATTCTGCCTGGGTGCCGTacac > 1:1430428/1‑54 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCt < 1:2792172/71‑1 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTATGCCTGGGTGCCGTACACCCA‑TTTTCAta > 1:1193992/1‑64 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCg > 1:2710033/1‑66 (MQ=255) aCGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTAc > 1:483846/1‑69 (MQ=255) gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:2573694/1‑37 (MQ=255) gCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCa < 1:872736/57‑1 (MQ=255) gATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTa > 1:651933/1‑70 (MQ=255) gATGT‑CCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaaa < 1:667737/71‑1 (MQ=255) aTGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaaa < 1:3153832/71‑1 (MQ=255) tCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGt < 1:2497198/71‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGGCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTgg > 1:3355659/1‑71 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGAt > 1:683974/1‑53 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCATGGTaa < 1:2600200/65‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTgg > 1:205432/1‑71 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTgg < 1:3386711/71‑1 (MQ=255) ccGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTg < 1:1167861/70‑1 (MQ=255) gTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAggg > 1:2070441/1‑60 (MQ=255) gTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTa > 1:3089411/1‑71 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTa > 1:1457966/1‑53 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaaa > 1:1462377/1‑63 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTAt > 1:523469/1‑71 (MQ=255) tCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcg > 1:3752459/1‑71 (MQ=255) cACTTTGACTCCGCGTGGGTGCCTTACACCAACTTCTCA‑CCGATTTACGAAGGTAAATGCGGTATGAgcgg < 1:3016437/71‑1 (MQ=255) cACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcg < 1:2506511/70‑1 (MQ=255) aCTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcggc < 1:1098767/71‑1 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGAt > 1:1972281/1‑39 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAg > 1:647477/1‑63 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGc > 1:578701/1‑71 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGc > 1:3855481/1‑71 (MQ=255) gATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTAt < 1:3514064/59‑1 (MQ=255) cTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAg < 1:3432581/65‑1 (MQ=255) | TGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGC > minE/189430‑189560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |