| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,269 | A→G | 8.1% | intergenic (‑245/+159) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,269 | 0 | A | G | 8.1% | 205.8 / 7.8 | 74 | intergenic (‑245/+159) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (32/36); new base G (3/3); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATT > minE/822201‑822338 | cGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:1808010/1‑71 (MQ=39) ggCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACAt < 1:168253/71‑1 (MQ=39) cTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc < 1:1373289/71‑1 (MQ=39) ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTcc > 1:819932/1‑71 (MQ=39) ggTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTcc < 1:458069/69‑1 (MQ=40) ggTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:2280375/1‑71 (MQ=39) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGc < 1:1834663/71‑1 (MQ=39) taTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt < 1:1831327/69‑1 (MQ=255) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2201998/71‑1 (MQ=39) aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCa < 1:3503504/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa < 1:582289/71‑1 (MQ=39) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa < 1:3813511/71‑1 (MQ=39) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCttt > 1:3703888/1‑63 (MQ=40) acGTCGCAATTCATCTTAACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCttt > 1:153315/1‑63 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCGTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt > 1:3787728/1‑71 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:366524/1‑58 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg > 1:2636052/1‑58 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa > 1:2580893/1‑68 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACa > 1:184691/1‑68 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg > 1:1772258/1‑69 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt < 1:3229484/71‑1 (MQ=39) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCt < 1:3704765/71‑1 (MQ=39) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTg < 1:3484285/70‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGt < 1:1046257/71‑1 (MQ=39) ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCggtt < 1:2703103/52‑3 (MQ=255) ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGt < 1:530263/71‑1 (MQ=34) tCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAg > 1:1907939/1‑60 (MQ=11) tCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAg > 1:1849298/1‑60 (MQ=11) ttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGc < 1:193888/56‑1 (MQ=11) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCaa < 1:1492259/50‑1 (MQ=11) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGttgc < 1:3203528/69‑3 (MQ=255) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGtt < 1:1881329/67‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTc < 1:1308077/69‑1 (MQ=40) ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:3249432/56‑1 (MQ=35) tcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGtt < 1:2846250/63‑1 (MQ=40) caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGtta > 1:2533098/1‑53 (MQ=11) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:2377031/1‑45 (MQ=38) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg > 1:1580054/1‑48 (MQ=38) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAgg > 1:1188287/1‑71 (MQ=255) ccTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtgt < 1:3540193/71‑1 (MQ=255) ccTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtgt > 1:3490891/1‑71 (MQ=255) ccTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtg < 1:718169/70‑1 (MQ=255) ccTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtg < 1:2167625/70‑1 (MQ=255) cTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGtgtg < 1:3909709/71‑1 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTgg < 1:1173134/69‑1 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGt < 1:617587/70‑1 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGt < 1:151232/70‑1 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa > 1:3236550/1‑71 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa > 1:1960187/1‑71 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa < 1:1221587/71‑1 (MQ=255) aaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGtt > 1:421502/1‑44 (MQ=38) aaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGtt > 1:222464/1‑44 (MQ=38) aTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACa < 1:3769323/71‑1 (MQ=255) tttGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGc > 1:1706967/1‑71 (MQ=255) ttGCAATGAAGAGTACATTCCGGCTTTTTAACAGCTGTTGTAGTGGTTTCGTGCAGGGTGTTGTACAGGCt > 1:2992040/1‑71 (MQ=21) tGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGtt > 1:1011061/1‑38 (MQ=40) tGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTc > 1:3688529/1‑71 (MQ=255) tGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTc > 1:2267544/1‑71 (MQ=255) cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg < 1:797970/40‑1 (MQ=40) aaTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgt > 1:1393725/1‑55 (MQ=255) aaTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTgg > 1:3261759/1‑59 (MQ=255) aaTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGc > 1:3749856/1‑70 (MQ=255) aaTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGc > 1:3857699/1‑70 (MQ=255) aaTGAAGAGTACATTCCGCCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTgg > 1:1631771/1‑59 (MQ=255) aTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTAc < 1:2618971/61‑1 (MQ=255) tGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAAt < 1:263028/71‑1 (MQ=255) aaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg > 1:3129859/1‑45 (MQ=255) aaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtg < 1:525217/52‑1 (MQ=255) agTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGa < 1:2097700/71‑1 (MQ=255) gTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgt > 1:1829139/1‑47 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAg < 1:1185521/55‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAg < 1:6262/55‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAtt < 1:3868239/71‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTATCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAt > 1:500221/1‑70 (MQ=255) | CGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATT > minE/822201‑822338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |