| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,094 | C→T | 15.0% | D184D (GAC→GAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,094 | 0 | C | T | 15.0% | 150.0 / 14.1 | 60 | D184D (GAC→GAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base T (2/7); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTC > minE/832025‑832163 | ttcCTGGCAGGAGGTGAACGAACTGATCTCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGAcc > 1:2541407/1‑71 (MQ=255) tcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCg > 1:1909914/1‑71 (MQ=255) ccTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgt > 1:677371/1‑71 (MQ=255) cTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:2900557/71‑1 (MQ=255) ggCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGtt > 1:119835/1‑71 (MQ=255) gCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtgt < 1:2940158/69‑1 (MQ=255) gCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTg < 1:196690/71‑1 (MQ=255) gaggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTgg < 1:2809470/71‑1 (MQ=255) gTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCTGACCGTGTTGCTGGt < 1:3855664/69‑1 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTgg > 1:788178/1‑66 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGttt < 1:351880/69‑1 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:2537009/58‑1 (MQ=255) tGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAtt > 1:1041434/1‑71 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGttt < 1:2612761/58‑1 (MQ=255) tgccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCgg > 1:935703/2‑71 (MQ=255) cgccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGAcc > 1:634622/1‑44 (MQ=255) cgccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCg > 1:1298201/1‑70 (MQ=255) cgccgcATCTAACGTTTACACCATCAAAAACTACGGCCCCGACCGTGTTGCTGGTTTCTCGCCAATTCCgg > 1:3104682/1‑71 (MQ=255) gccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCgg < 1:3031776/70‑1 (MQ=255) gcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAAt < 1:2560064/71‑1 (MQ=255) gcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAAt < 1:2572548/71‑1 (MQ=255) cATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATg > 1:3678561/1‑71 (MQ=255) cgcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGt < 1:636593/69‑1 (MQ=255) cgcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGt < 1:3671646/69‑1 (MQ=255) cTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc > 1:3169106/1‑70 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCg < 1:3300731/68‑1 (MQ=255) ttACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGtt > 1:1988771/1‑70 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGttt < 1:866329/70‑1 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTc > 1:3519133/1‑71 (MQ=255) gacCATCAAAACTTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGttt < 1:2066270/67‑1 (MQ=255) gacCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCtt < 1:1990199/70‑1 (MQ=255) cacCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt < 1:1292348/71‑1 (MQ=255) acCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCt < 1:3504019/69‑1 (MQ=255) acCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:3564092/1‑71 (MQ=255) ccATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTAc < 1:3482110/71‑1 (MQ=255) cATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACg < 1:2068268/71‑1 (MQ=255) aaaacCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGccg < 1:757389/70‑3 (MQ=255) aaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:2730605/1‑47 (MQ=255) aaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:2802201/1‑47 (MQ=255) aaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCgg < 1:3312136/71‑1 (MQ=255) aaaCTACGGCCCGGACCGTGTTGCTGGTTTATCGCCAATTCCGGCaa > 1:942336/1‑47 (MQ=255) aaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGcc > 1:1054923/1‑35 (MQ=255) aaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCggg < 1:3059410/71‑1 (MQ=255) aaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCgg > 1:371405/1‑70 (MQ=255) cTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt < 1:2516922/59‑1 (MQ=255) aCGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:204929/1‑58 (MQ=255) cGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGtt > 1:1641533/1‑52 (MQ=255) cGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCa < 1:1010444/70‑1 (MQ=255) ggCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGa > 1:2791558/1‑46 (MQ=255) ggCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGa > 1:3263008/1‑46 (MQ=255) gCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGtt < 1:2548387/50‑1 (MQ=255) ccGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTa > 1:436276/1‑71 (MQ=255) ggACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtc > 1:3637623/1‑71 (MQ=255) ggACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtc > 1:616363/1‑71 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc < 1:2185862/38‑1 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATgg > 1:1314347/1‑43 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATg < 1:1062666/42‑1 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATg < 1:2907780/42‑1 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATg < 1:954096/42‑1 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtct < 1:1066680/71‑1 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtct < 1:1695461/71‑1 (MQ=255) aCCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctc < 1:14805/71‑1 (MQ=255) | TTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTC > minE/832025‑832163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |