Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,170 A→C 14.1% I210L (ATT→CTT)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1700AC14.1% 200.5 / 36.2 99I210L (ATT→CTT) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (48/37);  new base C (6/8);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 104 total aligned reads displayed.

GCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGG  >  minE/832101‑832240
                                                                     |                                                                      
gCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                       >  1:3524794/1‑71 (MQ=255)
 ccggTTTCTCGCCGATCCCGGCTATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCtt                                                                      <  1:1692044/69‑1 (MQ=255)
 cTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAtt                                                                      <  1:276185/71‑1 (MQ=255)
 cTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                       <  1:3205353/70‑1 (MQ=255)
  cggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTg                                                                     <  1:1763539/70‑1 (MQ=255)
    gTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGc                                                                   <  1:1921210/71‑1 (MQ=255)
    gTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                        >  1:688888/1‑66 (MQ=255)
    gTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                        >  1:1134028/1‑66 (MQ=255)
    gTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGc                                                                   <  1:3392997/71‑1 (MQ=255)
       tctcGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGATATCTCTCGCTGATTGGCgg                                                                 >  1:503240/1‑70 (MQ=255)
        ctcGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTa                                                               >  1:34490/1‑71 (MQ=255)
           gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCAcct                                                            <  1:2778830/71‑3 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                        >  1:754515/1‑59 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                              >  1:3864520/1‑69 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                              >  1:1179512/1‑69 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            <  1:3831561/71‑1 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            <  1:741763/71‑1 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            <  1:604534/71‑1 (MQ=255)
           gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            >  1:2991987/1‑71 (MQ=255)
            ccAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTg                                                           >  1:2483212/1‑71 (MQ=255)
             cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                          >  1:3388541/1‑71 (MQ=255)
             cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                          <  1:3323066/71‑1 (MQ=255)
              aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTgg                                                                    >  1:3641737/1‑60 (MQ=255)
              aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                         >  1:1457850/1‑71 (MQ=255)
                ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                         >  1:3323626/1‑69 (MQ=255)
                ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGAGGTACTTGCtt                                                        >  1:3808029/1‑70 (MQ=255)
                 tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                        >  1:2817410/1‑69 (MQ=255)
                   cGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                              <  1:3705275/61‑1 (MQ=255)
                   cGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                     <  1:2844012/70‑1 (MQ=255)
                    ggCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAgtt                                                   <  1:2549326/71‑3 (MQ=255)
                     gcgaTGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGttt                                                  >  1:3752547/4‑71 (MQ=255)
                     gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            <  1:3367791/61‑1 (MQ=255)
                     gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                            <  1:2404714/61‑1 (MQ=255)
                     gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                  >  1:2286859/1‑71 (MQ=255)
                      cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                  >  1:3806575/1‑70 (MQ=255)
                       aaTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTgg                                                          >  1:816553/1‑60 (MQ=255)
                         tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                        <  1:2568209/45‑1 (MQ=255)
                          gTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTaa                                                      <  1:3395008/62‑1 (MQ=255)
                           tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                            <  1:1302671/71‑1 (MQ=255)
                              aTGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTg                                         <  1:1520559/71‑1 (MQ=255)
                              aTGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                           <  1:2846263/69‑1 (MQ=255)
                               tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                       >  1:2253290/1‑40 (MQ=255)
                               tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                       >  1:2485685/1‑40 (MQ=255)
                                ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                      >  1:83013/1‑56 (MQ=255)
                                ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                      >  1:3128065/1‑56 (MQ=255)
                                ggTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                          >  1:1223452/1‑52 (MQ=255)
                                    tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                             >  1:1491344/1‑45 (MQ=255)
                                    tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                    <  1:1480589/70‑1 (MQ=255)
                                    tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                   >  1:2595552/1‑71 (MQ=255)
                                      ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                 <  1:585165/55‑1 (MQ=255)
                                        aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                  <  1:1296926/68‑1 (MQ=255)
                                        aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                <  1:2233866/70‑1 (MQ=255)
                                        aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                <  1:1987235/70‑1 (MQ=255)
                                         cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                               <  1:3524280/70‑1 (MQ=255)
                                         cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                              <  1:2150949/71‑1 (MQ=255)
                                         cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                              <  1:1830429/71‑1 (MQ=255)
                                          gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                             >  1:1258687/1‑71 (MQ=255)
                                          gCACCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                              >  1:3469226/1‑54 (MQ=255)
                                           cATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                      <  1:1785476/61‑1 (MQ=255)
                                            aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                           >  1:396847/1‑71 (MQ=255)
                                              cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                          >  1:246326/1‑70 (MQ=255)
                                               gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                        <  1:3345869/71‑1 (MQ=255)
                                               gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                        <  1:2526776/71‑1 (MQ=255)
                                               gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                        >  1:2472942/1‑71 (MQ=255)
                                                ggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACCTGcctcc                       >  1:2531308/1‑71 (MQ=255)
                                                 gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGTTTCTACGACTGGTACTGCGACTTGcctcct                      <  1:2330743/71‑1 (MQ=255)
                                                  tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                              <  1:3922766/62‑1 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                            >  1:3525635/1‑46 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                             >  1:1317014/1‑61 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                   >  1:1408826/1‑71 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                   <  1:2010019/71‑1 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                   >  1:2483430/1‑71 (MQ=255)
                                                    cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                   >  1:2843832/1‑71 (MQ=255)
                                                      cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                  >  1:89341/1‑38 (MQ=255)
                                                      cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                   >  1:1523585/1‑37 (MQ=255)
                                                      cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                 <  1:1525087/71‑1 (MQ=255)
                                                      cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                 >  1:1970190/1‑71 (MQ=255)
                                                      cGCTATCTCTAGCTGATTGGCGGTACTTGCTTAAGCtt                                                  >  1:3912387/1‑38 (MQ=255)
                                                       gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                >  1:1003353/1‑71 (MQ=255)
                                                       gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                >  1:510064/1‑71 (MQ=255)
                                                       gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                >  1:3778550/1‑71 (MQ=255)
                                                       gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                >  1:3162483/1‑71 (MQ=255)
                                                       gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                 >  1:2269785/1‑70 (MQ=255)
                                                        cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc               <  1:1105305/71‑1 (MQ=255)
                                                          aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                    >  1:3501843/1‑64 (MQ=255)
                                                          aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc              >  1:3292544/1‑70 (MQ=255)
                                                          aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                           <  1:1050555/41‑1 (MQ=255)
                                                           tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc              <  1:3875853/69‑1 (MQ=255)
                                                            ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc            >  1:2059903/1‑70 (MQ=255)
                                                            ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc            >  1:1599583/1‑70 (MQ=255)
                                                            ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc            >  1:1034909/1‑70 (MQ=255)
                                                              gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat           >  1:214126/2‑67 (MQ=255)
                                                               tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa          <  1:2128473/69‑1 (MQ=255)
                                                               tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc        >  1:3680238/1‑71 (MQ=255)
                                                               tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc        >  1:2177040/1‑71 (MQ=255)
                                                                 gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc               <  1:3811769/62‑1 (MQ=255)
                                                                  cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg     <  1:3590913/71‑1 (MQ=255)
                                                                  cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg     <  1:1659438/71‑1 (MQ=255)
                                                                    gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg   >  1:1951126/1‑71 (MQ=255)
                                                                     aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg  >  1:3435915/1‑71 (MQ=255)
                                                                     |                                                                      
GCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGG  >  minE/832101‑832240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: