Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,181 T→C 12.6% T213T (ACT→ACC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1810TC12.6% 224.3 / 20.4 87T213T (ACT→ACCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (7/4);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACT  >  minE/832112‑832250
                                                                     |                                                                     
gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      >  1:2991987/1‑71 (MQ=255)
gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      <  1:3831561/71‑1 (MQ=255)
gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      <  1:604534/71‑1 (MQ=255)
gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      <  1:741763/71‑1 (MQ=255)
 ccAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTg                                                                     >  1:2483212/1‑71 (MQ=255)
  cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                    <  1:3323066/71‑1 (MQ=255)
  cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                    >  1:3388541/1‑71 (MQ=255)
   aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                   >  1:1457850/1‑71 (MQ=255)
     ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                   >  1:3323626/1‑69 (MQ=255)
     ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGAGGTACTTGCtt                                                                  >  1:3808029/1‑70 (MQ=255)
      tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  >  1:2817410/1‑69 (MQ=255)
        cGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                               <  1:2844012/70‑1 (MQ=255)
         ggCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAgtt                                                             <  1:2549326/71‑3 (MQ=255)
          gcgaTGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGttt                                                            >  1:3752547/4‑71 (MQ=255)
          gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      <  1:3367791/61‑1 (MQ=255)
          gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACtt                                                                      <  1:2404714/61‑1 (MQ=255)
          gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:2286859/1‑71 (MQ=255)
           cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:3806575/1‑70 (MQ=255)
            aaTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTgg                                                                    >  1:816553/1‑60 (MQ=255)
               gTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTaa                                                                <  1:3395008/62‑1 (MQ=255)
                tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                      <  1:1302671/71‑1 (MQ=255)
                   aTGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTg                                                   <  1:1520559/71‑1 (MQ=255)
                   aTGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                                     <  1:2846263/69‑1 (MQ=255)
                     ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                                >  1:83013/1‑56 (MQ=255)
                     ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                                >  1:3128065/1‑56 (MQ=255)
                     ggTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                    >  1:1223452/1‑52 (MQ=255)
                         tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                                       >  1:1491344/1‑45 (MQ=255)
                         tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                              <  1:1480589/70‑1 (MQ=255)
                         tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                             >  1:2595552/1‑71 (MQ=255)
                           ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                           <  1:585165/55‑1 (MQ=255)
                             aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                            <  1:1296926/68‑1 (MQ=255)
                             aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                          <  1:2233866/70‑1 (MQ=255)
                             aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                          <  1:1987235/70‑1 (MQ=255)
                              cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                         <  1:3524280/70‑1 (MQ=255)
                              cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                        <  1:2150949/71‑1 (MQ=255)
                              cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                        <  1:1830429/71‑1 (MQ=255)
                               gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       >  1:1258687/1‑71 (MQ=255)
                               gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       >  1:917353/1‑71 (MQ=255)
                               gCACCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                        >  1:3469226/1‑54 (MQ=255)
                                cATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                <  1:1785476/61‑1 (MQ=255)
                                 aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                                     >  1:396847/1‑71 (MQ=255)
                                   cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                    >  1:246326/1‑70 (MQ=255)
                                    gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                  <  1:2526776/71‑1 (MQ=255)
                                    gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                  <  1:3345869/71‑1 (MQ=255)
                                    gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                  >  1:2472942/1‑71 (MQ=255)
                                    gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcccc                                  <  1:1947401/71‑3 (MQ=255)
                                     ggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACCTGcctcc                                 >  1:2531308/1‑71 (MQ=255)
                                      gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGTTTCTACGACTGGTACTGCGACTTGcctcct                                <  1:2330743/71‑1 (MQ=255)
                                       tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                         <  1:3529190/61‑1 (MQ=255)
                                       tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                        <  1:3922766/62‑1 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                      >  1:3525635/1‑46 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       >  1:1317014/1‑61 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                             >  1:2843832/1‑71 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                             <  1:2010019/71‑1 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                             >  1:1408826/1‑71 (MQ=255)
                                         cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                             >  1:2483430/1‑71 (MQ=255)
                                           cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:89341/1‑38 (MQ=255)
                                           cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                             >  1:1523585/1‑37 (MQ=255)
                                           cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                           >  1:1970190/1‑71 (MQ=255)
                                           cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                           <  1:1525087/71‑1 (MQ=255)
                                           cGCTATCTCTAGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:3912387/1‑38 (MQ=255)
                                            gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                          >  1:3778550/1‑71 (MQ=255)
                                            gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                          >  1:1003353/1‑71 (MQ=255)
                                            gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                          >  1:3162483/1‑71 (MQ=255)
                                            gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                          >  1:510064/1‑71 (MQ=255)
                                            gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                           >  1:2269785/1‑70 (MQ=255)
                                             cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                         <  1:1105305/71‑1 (MQ=255)
                                               aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                              >  1:3501843/1‑64 (MQ=255)
                                               aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                        >  1:3292544/1‑70 (MQ=255)
                                               aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                        <  1:2645165/70‑1 (MQ=255)
                                               aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                                     <  1:1050555/41‑1 (MQ=255)
                                                tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                        <  1:3875853/69‑1 (MQ=255)
                                                 ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                      >  1:1034909/1‑70 (MQ=255)
                                                 ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                      >  1:1599583/1‑70 (MQ=255)
                                                 ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                      >  1:2059903/1‑70 (MQ=255)
                                                   gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat                     >  1:214126/2‑67 (MQ=255)
                                                    tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                    <  1:2128473/69‑1 (MQ=255)
                                                    tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                  >  1:3680238/1‑71 (MQ=255)
                                                    tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                  >  1:2177040/1‑71 (MQ=255)
                                                      gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                         <  1:3811769/62‑1 (MQ=255)
                                                       cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg               <  1:3590913/71‑1 (MQ=255)
                                                       cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg               <  1:1659438/71‑1 (MQ=255)
                                                         gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg             >  1:1951126/1‑71 (MQ=255)
                                                          aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg            >  1:3435915/1‑71 (MQ=255)
                                                           ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc           >  1:2810613/1‑71 (MQ=255)
                                                           ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc           <  1:3029009/71‑1 (MQ=255)
                                                            ttgCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg          <  1:3865011/69‑1 (MQ=255)
                                                              gCGGTACTTGCTTAAGCTTCTACGACTGGTACAGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAg        <  1:2912485/71‑1 (MQ=255)
                                                              gCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAg        >  1:1652358/1‑71 (MQ=255)
                                                                ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                  <  1:3037837/43‑1 (MQ=255)
                                                                ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCa                     >  1:2643338/1‑56 (MQ=255)
                                                                 gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa      >  1:3821945/1‑70 (MQ=255)
                                                                  tACTTGCTTAAGCTTCTACGACTCGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg             <  1:869607/62‑1 (MQ=255)
                                                                    cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt  <  1:3917703/71‑1 (MQ=255)
                                                                     ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                   >  1:2709945/1‑53 (MQ=255)
                                                                     |                                                                     
GCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACT  >  minE/832112‑832250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: