Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,184 C→T 12.9% C214C (TGC→TGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1840CT12.9% 225.8 / 18.2 85C214C (TGC→TGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (40/34);  new base T (7/4);  total (47/38)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACG  >  minE/832114‑832254
                                                                      |                                                                      
cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                        <  1:3323066/71‑1 (MQ=255)
cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                        >  1:3388541/1‑71 (MQ=255)
 aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                       >  1:1457850/1‑71 (MQ=255)
   ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                       >  1:3323626/1‑69 (MQ=255)
   ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGAGGTACTTGCtt                                                                      >  1:3808029/1‑70 (MQ=255)
    tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      >  1:2817410/1‑69 (MQ=255)
      cGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                                   <  1:2844012/70‑1 (MQ=255)
       ggCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAgtt                                                                 <  1:2549326/71‑3 (MQ=255)
        gcgaTGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGttt                                                                >  1:3752547/4‑71 (MQ=255)
        gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:2286859/1‑71 (MQ=255)
         cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:3806575/1‑70 (MQ=255)
             gTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTaa                                                                    <  1:3395008/62‑1 (MQ=255)
              tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                          <  1:1302671/71‑1 (MQ=255)
                 aTGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTg                                                       <  1:1520559/71‑1 (MQ=255)
                 aTGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                                         <  1:2846263/69‑1 (MQ=255)
                   ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                                    >  1:3128065/1‑56 (MQ=255)
                   ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTaa                                                                    >  1:83013/1‑56 (MQ=255)
                   ggTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGc                                                                        >  1:1223452/1‑52 (MQ=255)
                       tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                                  <  1:1480589/70‑1 (MQ=255)
                       tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                                 >  1:2595552/1‑71 (MQ=255)
                         ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               <  1:585165/55‑1 (MQ=255)
                           aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                                <  1:1296926/68‑1 (MQ=255)
                           aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                              <  1:2233866/70‑1 (MQ=255)
                           aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGa                                              <  1:1987235/70‑1 (MQ=255)
                            cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                             <  1:3524280/70‑1 (MQ=255)
                            cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                            <  1:2150949/71‑1 (MQ=255)
                            cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                            <  1:1830429/71‑1 (MQ=255)
                             gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           >  1:917353/1‑71 (MQ=255)
                             gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           >  1:1258687/1‑71 (MQ=255)
                             gCACCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                            >  1:3469226/1‑54 (MQ=255)
                              cATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    <  1:1785476/61‑1 (MQ=255)
                               aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                                         >  1:396847/1‑71 (MQ=255)
                                 cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                        >  1:246326/1‑70 (MQ=255)
                                  gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      >  1:2472942/1‑71 (MQ=255)
                                  gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      <  1:2526776/71‑1 (MQ=255)
                                  gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      <  1:3345869/71‑1 (MQ=255)
                                  gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcccc                                      <  1:1947401/71‑3 (MQ=255)
                                   ggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACCTGcctcc                                     >  1:2531308/1‑71 (MQ=255)
                                    gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGTTTCTACGACTGGTACTGCGACTTGcctcct                                    <  1:2330743/71‑1 (MQ=255)
                                     tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                             <  1:3529190/61‑1 (MQ=255)
                                     tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                            <  1:3922766/62‑1 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                          >  1:3525635/1‑46 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           >  1:1317014/1‑61 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                 <  1:2010019/71‑1 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                 >  1:1408826/1‑71 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                 >  1:2843832/1‑71 (MQ=255)
                                       cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                 >  1:2483430/1‑71 (MQ=255)
                                         cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:89341/1‑38 (MQ=255)
                                         cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                                 >  1:1523585/1‑37 (MQ=255)
                                         cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                               <  1:1525087/71‑1 (MQ=255)
                                         cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                               >  1:1970190/1‑71 (MQ=255)
                                         cGCTATCTCTAGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:3912387/1‑38 (MQ=255)
                                          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                              >  1:510064/1‑71 (MQ=255)
                                          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                              >  1:3162483/1‑71 (MQ=255)
                                          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                              >  1:3778550/1‑71 (MQ=255)
                                          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                              >  1:1003353/1‑71 (MQ=255)
                                          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                               >  1:2269785/1‑70 (MQ=255)
                                           cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                             <  1:1105305/71‑1 (MQ=255)
                                             aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                                  >  1:3501843/1‑64 (MQ=255)
                                             aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                            <  1:2645165/70‑1 (MQ=255)
                                             aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                            >  1:3292544/1‑70 (MQ=255)
                                             aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGAc                                                         <  1:1050555/41‑1 (MQ=255)
                                              tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                            <  1:3875853/69‑1 (MQ=255)
                                               ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                          >  1:1034909/1‑70 (MQ=255)
                                               ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                          >  1:1599583/1‑70 (MQ=255)
                                               ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                          >  1:2059903/1‑70 (MQ=255)
                                                 gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat                         >  1:214126/2‑67 (MQ=255)
                                                  tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                        <  1:2128473/69‑1 (MQ=255)
                                                  tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                      >  1:2177040/1‑71 (MQ=255)
                                                  tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                      >  1:3680238/1‑71 (MQ=255)
                                                    gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                             <  1:3811769/62‑1 (MQ=255)
                                                     cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                   <  1:3590913/71‑1 (MQ=255)
                                                     cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                   <  1:1659438/71‑1 (MQ=255)
                                                       gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                 >  1:1951126/1‑71 (MQ=255)
                                                        aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg                >  1:3435915/1‑71 (MQ=255)
                                                         ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc               <  1:3029009/71‑1 (MQ=255)
                                                         ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc               >  1:2810613/1‑71 (MQ=255)
                                                          ttgCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg              <  1:3865011/69‑1 (MQ=255)
                                                            gCGGTACTTGCTTAAGCTTCTACGACTGGTACAGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAg            <  1:2912485/71‑1 (MQ=255)
                                                            gCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAg            >  1:1652358/1‑71 (MQ=255)
                                                              ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      <  1:3037837/43‑1 (MQ=255)
                                                              ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCa                         >  1:2643338/1‑56 (MQ=255)
                                                               gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa          >  1:3821945/1‑70 (MQ=255)
                                                                tACTTGCTTAAGCTTCTACGACTCGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                 <  1:869607/62‑1 (MQ=255)
                                                                  cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt      <  1:3917703/71‑1 (MQ=255)
                                                                   ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                       >  1:2709945/1‑53 (MQ=255)
                                                                    tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                              >  1:247265/1‑45 (MQ=255)
                                                                     gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg     <  1:700467/69‑1 (MQ=255)
                                                                      cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg     <  1:1064268/68‑1 (MQ=255)
                                                                      cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg  <  1:1482933/71‑1 (MQ=255)
                                                                      |                                                                      
CAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACG  >  minE/832114‑832254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: