Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,212 T→C 23.5% L224L (TTG→CTG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,2120TC23.5% 153.9 / 48.4 85L224L (TTG→CTG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (31/34);  new base C (11/9);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTC  >  minE/832142‑832282
                                                                      |                                                                      
cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                                                        <  1:2150949/71‑1 (MQ=255)
cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                                                        <  1:1830429/71‑1 (MQ=255)
 gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                                                       >  1:917353/1‑71 (MQ=255)
 gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                                                       >  1:1258687/1‑71 (MQ=255)
   aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                                                                     >  1:396847/1‑71 (MQ=255)
     cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                                                    >  1:246326/1‑70 (MQ=255)
      gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                  <  1:3345869/71‑1 (MQ=255)
      gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                  <  1:2526776/71‑1 (MQ=255)
      gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                  >  1:2472942/1‑71 (MQ=255)
      gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcccc                                                                  <  1:1947401/71‑3 (MQ=255)
       ggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACCTGcctcc                                                                 >  1:2531308/1‑71 (MQ=255)
        gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGTTTCTACGACTGGTACTGCGACTTGcctcct                                                                <  1:2330743/71‑1 (MQ=255)
         tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                                                        <  1:3922766/62‑1 (MQ=255)
           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                                                       >  1:1317014/1‑61 (MQ=255)
           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                             <  1:2010019/71‑1 (MQ=255)
           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                             >  1:2483430/1‑71 (MQ=255)
           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                             >  1:1408826/1‑71 (MQ=255)
           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                             >  1:2843832/1‑71 (MQ=255)
             cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                           <  1:1525087/71‑1 (MQ=255)
             cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                           >  1:1970190/1‑71 (MQ=255)
              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          >  1:3162483/1‑71 (MQ=255)
              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          >  1:3778550/1‑71 (MQ=255)
              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          >  1:510064/1‑71 (MQ=255)
              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          >  1:1003353/1‑71 (MQ=255)
              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                           >  1:2269785/1‑70 (MQ=255)
               cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                         <  1:1105305/71‑1 (MQ=255)
                 aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                                                              >  1:3501843/1‑64 (MQ=255)
                 aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                        >  1:3292544/1‑70 (MQ=255)
                 aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                        <  1:2645165/70‑1 (MQ=255)
                  tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                        <  1:3875853/69‑1 (MQ=255)
                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                      >  1:2059903/1‑70 (MQ=255)
                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                      >  1:1034909/1‑70 (MQ=255)
                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                      >  1:1599583/1‑70 (MQ=255)
                     gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat                                                     >  1:214126/2‑67 (MQ=255)
                      tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                    <  1:2128473/69‑1 (MQ=255)
                      tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                                                  >  1:3680238/1‑71 (MQ=255)
                      tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                                                  >  1:2177040/1‑71 (MQ=255)
                        gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                         <  1:3811769/62‑1 (MQ=255)
                         cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                               <  1:3590913/71‑1 (MQ=255)
                         cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                               <  1:1659438/71‑1 (MQ=255)
                           gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                                             >  1:1951126/1‑71 (MQ=255)
                            aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg                                            >  1:3435915/1‑71 (MQ=255)
                             ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc                                           <  1:3029009/71‑1 (MQ=255)
                             ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc                                           >  1:2810613/1‑71 (MQ=255)
                              ttgCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg                                          <  1:3865011/69‑1 (MQ=255)
                                gCGGTACTTGCTTAAGCTTCTACGACTGGTACAGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAg                                        <  1:2912485/71‑1 (MQ=255)
                                gCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAg                                        >  1:1652358/1‑71 (MQ=255)
                                  ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                  <  1:3037837/43‑1 (MQ=255)
                                  ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCa                                                     >  1:2643338/1‑56 (MQ=255)
                                   gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa                                      >  1:3821945/1‑70 (MQ=255)
                                    tACTTGCTTAAGCTTCTACGACTCGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                                             <  1:869607/62‑1 (MQ=255)
                                      cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt                                  <  1:3917703/71‑1 (MQ=255)
                                       ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                                                   >  1:2709945/1‑53 (MQ=255)
                                        tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          >  1:247265/1‑45 (MQ=255)
                                         gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg                                 <  1:700467/69‑1 (MQ=255)
                                          cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg                                 <  1:1064268/68‑1 (MQ=255)
                                          cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg                              <  1:1482933/71‑1 (MQ=255)
                                            tAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                                                              >  1:247908/1‑37 (MQ=16)
                                            tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa                            >  1:1842024/1‑71 (MQ=255)
                                             aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          <  1:824904/40‑1 (MQ=255)
                                               gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                         >  1:824643/1‑39 (MQ=255)
                                               gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                          <  1:2590855/38‑1 (MQ=255)
                                               gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                                                        >  1:2858709/1‑40 (MQ=255)
                                               gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                                                        >  1:81729/1‑40 (MQ=255)
                                                cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCTCAAACCTGGGGCGAGCAAACTGACGTACCgg                        <  1:375734/71‑1 (MQ=255)
                                                cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                               <  1:1304482/48‑1 (MQ=255)
                                                  tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGc                                           <  1:1338106/50‑1 (MQ=255)
                                                  tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCa                                      <  1:3075661/55‑1 (MQ=255)
                                                  tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa                       <  1:3881838/70‑1 (MQ=255)
                                                  tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa                       >  1:2231834/1‑70 (MQ=255)
                                                  tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa                      >  1:902386/1‑71 (MQ=255)
                                                   cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                    <  1:3819244/40‑1 (MQ=255)
                                                   cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                    <  1:2584082/40‑1 (MQ=255)
                                                   cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                       <  1:877986/69‑1 (MQ=255)
                                                   cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                     >  1:1349517/1‑71 (MQ=255)
                                                    tACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGa                                         >  1:2636449/1‑50 (MQ=255)
                                                    tACGACTGGTATTGCGACCTGCCGCCCGCATCGCCGATGACCTGGGGCg                                          <  1:2179961/49‑1 (MQ=255)
                                                     aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                     >  1:3906156/1‑69 (MQ=255)
                                                      cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctg                  >  1:2893140/1‑71 (MQ=255)
                                                      cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATct                   <  1:3163374/70‑1 (MQ=255)
                                                      cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                     <  1:3273029/68‑1 (MQ=255)
                                                         cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg                                          <  1:2402401/44‑1 (MQ=255)
                                                         cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg               <  1:2482226/71‑1 (MQ=255)
                                                              attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctgc                 <  1:3019215/62‑1 (MQ=255)
                                                              attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTgg          >  1:1164402/3‑71 (MQ=255)
                                                                tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                       <  1:3892563/56‑1 (MQ=255)
                                                                 gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                       <  1:3828782/55‑1 (MQ=255)
                                                                 gCGACTTGCCTCATGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa        >  1:1000755/1‑70 (MQ=255)
                                                                  cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc       <  1:1217644/70‑1 (MQ=255)
                                                                  cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc       <  1:3645032/70‑1 (MQ=255)
                                                                  cGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat       <  1:3768923/70‑2 (MQ=255)
                                                                      ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc  >  1:2335376/1‑71 (MQ=255)
                                                                      |                                                                      
CGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTC  >  minE/832142‑832282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: