Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,217 T→G 22.1% P225P (CCT→CCG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,2170TG22.1% 113.6 / 47.0 77P225P (CCT→CCGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base G (8/9);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCA  >  minE/832148‑832284
                                                                     |                                                                   
gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                    <  1:2526776/71‑1 (MQ=255)
gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                    >  1:2472942/1‑71 (MQ=255)
gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                    <  1:3345869/71‑1 (MQ=255)
 ggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACCTGcctcc                                                                   >  1:2531308/1‑71 (MQ=255)
  gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGTTTCTACGACTGGTACTGCGACTTGcctcct                                                                  <  1:2330743/71‑1 (MQ=255)
     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                               <  1:2010019/71‑1 (MQ=255)
     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                               >  1:2483430/1‑71 (MQ=255)
     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                               >  1:2843832/1‑71 (MQ=255)
     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCg                                                               >  1:1408826/1‑71 (MQ=255)
       cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                             <  1:1525087/71‑1 (MQ=255)
       cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                             >  1:1970190/1‑71 (MQ=255)
        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            >  1:3778550/1‑71 (MQ=255)
        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            >  1:1003353/1‑71 (MQ=255)
        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            >  1:510064/1‑71 (MQ=255)
        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            >  1:3162483/1‑71 (MQ=255)
        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                                                             >  1:2269785/1‑70 (MQ=255)
         cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                           <  1:1105305/71‑1 (MQ=255)
           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                                                                >  1:3501843/1‑64 (MQ=255)
           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                          <  1:2645165/70‑1 (MQ=255)
           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                          >  1:3292544/1‑70 (MQ=255)
            tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc                                                          <  1:3875853/69‑1 (MQ=255)
             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                        >  1:1034909/1‑70 (MQ=255)
             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                        >  1:2059903/1‑70 (MQ=255)
             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                                                        >  1:1599583/1‑70 (MQ=255)
               gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat                                                       >  1:214126/2‑67 (MQ=255)
                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                      <  1:2128473/69‑1 (MQ=255)
                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                                                    >  1:3680238/1‑71 (MQ=255)
                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                                                    >  1:2177040/1‑71 (MQ=255)
                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                           <  1:3811769/62‑1 (MQ=255)
                   cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                                 <  1:3590913/71‑1 (MQ=255)
                   cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                                 <  1:1659438/71‑1 (MQ=255)
                     gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                                               >  1:1951126/1‑71 (MQ=255)
                      aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg                                              >  1:3435915/1‑71 (MQ=255)
                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc                                             <  1:3029009/71‑1 (MQ=255)
                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc                                             >  1:2810613/1‑71 (MQ=255)
                        ttgCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg                                            <  1:3865011/69‑1 (MQ=255)
                          gCGGTACTTGCTTAAGCTTCTACGACTGGTACAGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAg                                          <  1:2912485/71‑1 (MQ=255)
                          gCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAg                                          >  1:1652358/1‑71 (MQ=255)
                            ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                                                    <  1:3037837/43‑1 (MQ=255)
                            ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCa                                                       >  1:2643338/1‑56 (MQ=255)
                             gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa                                        >  1:3821945/1‑70 (MQ=255)
                              tACTTGCTTAAGCTTCTACGACTCGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                                               <  1:869607/62‑1 (MQ=255)
                                cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt                                    <  1:3917703/71‑1 (MQ=255)
                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                                                     >  1:2709945/1‑53 (MQ=255)
                                  tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            >  1:247265/1‑45 (MQ=255)
                                   gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg                                   <  1:700467/69‑1 (MQ=255)
                                    cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg                                   <  1:1064268/68‑1 (MQ=255)
                                    cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg                                <  1:1482933/71‑1 (MQ=255)
                                      tAAGTTTCTACGACTGGTATTGCGACCTGccgcccgc                                                                >  1:247908/1‑37 (MQ=16)
                                      tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa                              >  1:1842024/1‑71 (MQ=255)
                                       aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            <  1:824904/40‑1 (MQ=255)
                                         gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                                                           >  1:824643/1‑39 (MQ=255)
                                         gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct                                                            <  1:2590855/38‑1 (MQ=255)
                                         gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                                                          >  1:81729/1‑40 (MQ=255)
                                         gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                                                          >  1:2858709/1‑40 (MQ=255)
                                          cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCTCAAACCTGGGGCGAGCAAACTGACGTACCgg                          <  1:375734/71‑1 (MQ=255)
                                          cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg                                                 <  1:1304482/48‑1 (MQ=255)
                                            tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGc                                             <  1:1338106/50‑1 (MQ=255)
                                            tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCa                                        <  1:3075661/55‑1 (MQ=255)
                                            tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa                         <  1:3881838/70‑1 (MQ=255)
                                            tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa                         >  1:2231834/1‑70 (MQ=255)
                                            tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa                        >  1:902386/1‑71 (MQ=255)
                                             cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                      <  1:2584082/40‑1 (MQ=255)
                                             cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                                                      <  1:3819244/40‑1 (MQ=255)
                                             cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                         <  1:877986/69‑1 (MQ=255)
                                             cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                       >  1:1349517/1‑71 (MQ=255)
                                              tACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGa                                           >  1:2636449/1‑50 (MQ=255)
                                              tACGACTGGTATTGCGACCTGCCGCCCGCATCGCCGATGACCTGGGGCg                                            <  1:2179961/49‑1 (MQ=255)
                                               aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                       >  1:3906156/1‑69 (MQ=255)
                                                cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctg                    >  1:2893140/1‑71 (MQ=255)
                                                cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATct                     <  1:3163374/70‑1 (MQ=255)
                                                cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt                       <  1:3273029/68‑1 (MQ=255)
                                                   cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg                                            <  1:2402401/44‑1 (MQ=255)
                                                   cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg                 <  1:2482226/71‑1 (MQ=255)
                                                        attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctgc                   <  1:3019215/62‑1 (MQ=255)
                                                        attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTgg            >  1:1164402/3‑71 (MQ=255)
                                                          tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                         <  1:3892563/56‑1 (MQ=255)
                                                           gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa                         <  1:3828782/55‑1 (MQ=255)
                                                           gCGACTTGCCTCATGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa          >  1:1000755/1‑70 (MQ=255)
                                                            cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc         <  1:1217644/70‑1 (MQ=255)
                                                            cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc         <  1:3645032/70‑1 (MQ=255)
                                                            cGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat         <  1:3768923/70‑2 (MQ=255)
                                                                ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc    >  1:2335376/1‑71 (MQ=255)
                                                                  gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg            <  1:2292016/61‑1 (MQ=255)
                                                                  gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg            <  1:313955/61‑1 (MQ=255)
                                                                   cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa  >  1:1639362/1‑70 (MQ=255)
                                                                     tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa  <  1:2042996/68‑1 (MQ=255)
                                                                     tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa  <  1:2338139/68‑1 (MQ=255)
                                                                     |                                                                   
GGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCA  >  minE/832148‑832284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: