| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,226 | T→G | 24.6% | S228S (TCT→TCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,226 | 0 | T | G | 24.6% | 87.0 / 47.4 | 69 | S228S (TCT→TCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/30); new base G (8/9); total (30/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCA > minE/832156‑832293 | gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct > 1:1003353/1‑71 (MQ=255)gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct > 1:3162483/1‑71 (MQ=255)gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct > 1:3778550/1‑71 (MQ=255)gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct > 1:510064/1‑71 (MQ=255) cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc < 1:1105305/71‑1 (MQ=255) aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc < 1:2645165/70‑1 (MQ=255) aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc > 1:3292544/1‑70 (MQ=255) tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGcc < 1:3875853/69‑1 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc > 1:1034909/1‑70 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc > 1:2059903/1‑70 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc > 1:1599583/1‑70 (MQ=255) gtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgat > 1:214126/2‑67 (MQ=255) tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa < 1:2128473/69‑1 (MQ=255) tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc > 1:2177040/1‑71 (MQ=255) tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc > 1:3680238/1‑71 (MQ=255) gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc < 1:3811769/62‑1 (MQ=255) cTGATTGGCTGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg < 1:3590913/71‑1 (MQ=255) cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg < 1:1659438/71‑1 (MQ=255) gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg > 1:1951126/1‑71 (MQ=255) aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTgggg > 1:3435915/1‑71 (MQ=255) ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc > 1:2810613/1‑71 (MQ=255) ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc < 1:3029009/71‑1 (MQ=255) ttgCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg < 1:3865011/69‑1 (MQ=255) gCGGTACTTGCTTAAGCTTCTACGACTGGTACAGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAg < 1:2912485/71‑1 (MQ=255) gCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAg > 1:1652358/1‑71 (MQ=255) ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCa > 1:2643338/1‑56 (MQ=255) gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa > 1:3821945/1‑70 (MQ=255) tACTTGCTTAAGCTTCTACGACTCGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg < 1:869607/62‑1 (MQ=255) cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt < 1:3917703/71‑1 (MQ=255) ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa > 1:2709945/1‑53 (MQ=255) tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct > 1:247265/1‑45 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg < 1:700467/69‑1 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg < 1:1064268/68‑1 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg < 1:1482933/71‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa > 1:1842024/1‑71 (MQ=255) aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct < 1:824904/40‑1 (MQ=255) gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc > 1:824643/1‑39 (MQ=255) gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtct < 1:2590855/38‑1 (MQ=255) gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:81729/1‑40 (MQ=255) gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:2858709/1‑40 (MQ=255) cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCTCAAACCTGGGGCGAGCAAACTGACGTACCgg < 1:375734/71‑1 (MQ=255) cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTg < 1:1304482/48‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGc < 1:1338106/50‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCa < 1:3075661/55‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa > 1:2231834/1‑70 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa < 1:3881838/70‑1 (MQ=255) tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa > 1:902386/1‑71 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa < 1:3819244/40‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa < 1:2584082/40‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:877986/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1349517/1‑71 (MQ=255) tACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGa > 1:2636449/1‑50 (MQ=255) tACGACTGGTATTGCGACCTGCCGCCCGCATCGCCGATGACCTGGGGCg < 1:2179961/49‑1 (MQ=255) aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:3906156/1‑69 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctg > 1:2893140/1‑71 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATct < 1:3163374/70‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt < 1:3273029/68‑1 (MQ=255) cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg < 1:2402401/44‑1 (MQ=255) cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:2482226/71‑1 (MQ=255) attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctgc < 1:3019215/62‑1 (MQ=255) attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTgg > 1:1164402/3‑71 (MQ=255) tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3892563/56‑1 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3828782/55‑1 (MQ=255) gCGACTTGCCTCATGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:1000755/1‑70 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1217644/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3645032/70‑1 (MQ=255) cGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat < 1:3768923/70‑2 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:2335376/1‑71 (MQ=255) gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:2292016/61‑1 (MQ=255) gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:313955/61‑1 (MQ=255) cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa > 1:1639362/1‑70 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2042996/68‑1 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2338139/68‑1 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAc < 1:2468480/71‑1 (MQ=255) gCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2493073/1‑71 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2353916/1‑70 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca < 1:3653117/69‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatca < 1:784003/71‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatc > 1:3764119/1‑70 (MQ=255) tctcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:971659/51‑1 (MQ=255) | GCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCA > minE/832156‑832293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |