| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,250 | T→C | 18.9% | T236T (ACT→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,250 | 0 | T | C | 18.9% | 155.7 / 30.6 | 74 | T236T (ACT→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/38); new base C (6/8); total (28/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGC > minE/832180‑832317 | cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt < 1:3917703/71‑1 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg < 1:700467/69‑1 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg < 1:1064268/68‑1 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg < 1:1482933/71‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa > 1:1842024/1‑71 (MQ=255) cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCTCAAACCTGGGGCGAGCAAACTGACGTACCgg < 1:375734/71‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa < 1:3881838/70‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa > 1:2231834/1‑70 (MQ=255) tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa > 1:902386/1‑71 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:877986/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1349517/1‑71 (MQ=255) aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:3906156/1‑69 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctg > 1:2893140/1‑71 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATct < 1:3163374/70‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt < 1:3273029/68‑1 (MQ=255) cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:2482226/71‑1 (MQ=255) attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATctgc < 1:3019215/62‑1 (MQ=255) attGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTgg > 1:1164402/3‑71 (MQ=255) tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3892563/56‑1 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3828782/55‑1 (MQ=255) gCGACTTGCCTCATGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:1000755/1‑70 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3645032/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1217644/70‑1 (MQ=255) cGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat < 1:3768923/70‑2 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:2335376/1‑71 (MQ=255) gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:313955/61‑1 (MQ=255) gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:2292016/61‑1 (MQ=255) cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa > 1:1639362/1‑70 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2338139/68‑1 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2042996/68‑1 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAc < 1:2468480/71‑1 (MQ=255) gCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2493073/1‑71 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2353916/1‑70 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca < 1:3653117/69‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatca < 1:784003/71‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatc > 1:3764119/1‑70 (MQ=255) tctcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:971659/51‑1 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCt > 1:2402554/1‑61 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:2893811/69‑1 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:3814001/1‑71 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:678182/42‑1 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:907856/1‑69 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt < 1:700540/71‑1 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt < 1:844787/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGTAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:2627818/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGTAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:1783589/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg > 1:457520/1‑71 (MQ=255) atgaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg < 1:634745/68‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgg < 1:2405470/71‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgg > 1:224929/1‑71 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:1740443/70‑1 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTggg > 1:2159452/2‑71 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:1945852/37‑1 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:403347/47‑1 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg > 1:1181728/1‑71 (MQ=255) aCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTgggg < 1:216741/71‑1 (MQ=255) cTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg > 1:3310098/1‑69 (MQ=255) tGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt > 1:2265544/1‑70 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3753141/70‑1 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3053453/70‑1 (MQ=255) gCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat < 1:1616344/38‑2 (MQ=37) gCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTat < 1:2570036/38‑2 (MQ=37) cGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg < 1:259794/71‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACg > 1:98572/1‑70 (MQ=255) gctaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:1355083/68‑1 (MQ=255) gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:2662689/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:618696/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc > 1:1545580/1‑71 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:1191578/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc > 1:1217330/1‑70 (MQ=255) aaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:2915844/63‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc < 1:3461661/71‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2409408/1‑63 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2568647/1‑63 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2502871/1‑69 (MQ=255) | CTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGC > minE/832180‑832317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |