| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,277 | C→T | 24.7% | Y245Y (TAC→TAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,277 | 0 | C | T | 24.7% | 145.0 / 46.9 | 81 | Y245Y (TAC→TAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/38); new base T (11/9); total (34/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTT > minE/832208‑832346 | cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3645032/70‑1 (MQ=255)cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1217644/70‑1 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:2335376/1‑71 (MQ=255) cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa > 1:1639362/1‑70 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2042996/68‑1 (MQ=255) tcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:2338139/68‑1 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAc < 1:2468480/71‑1 (MQ=255) gCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2493073/1‑71 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:2353916/1‑70 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca < 1:3653117/69‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatca < 1:784003/71‑1 (MQ=255) gTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatc > 1:3764119/1‑70 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCt > 1:2402554/1‑61 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:2893811/69‑1 (MQ=255) ccGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:3814001/1‑71 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:907856/1‑69 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt < 1:700540/71‑1 (MQ=255) gCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt < 1:844787/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGTAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:2627818/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGTAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:1783589/71‑1 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg > 1:457520/1‑71 (MQ=255) atgaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg < 1:634745/68‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgg > 1:224929/1‑71 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgg < 1:2405470/71‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:1740443/70‑1 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTggg > 1:2159452/2‑71 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:403347/47‑1 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg > 1:1181728/1‑71 (MQ=255) aCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTgggg < 1:216741/71‑1 (MQ=255) cTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg > 1:3310098/1‑69 (MQ=255) tGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt > 1:2265544/1‑70 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3053453/70‑1 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3753141/70‑1 (MQ=255) cGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg < 1:259794/71‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACg > 1:98572/1‑70 (MQ=255) gctaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:1355083/68‑1 (MQ=255) gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:2662689/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:618696/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:1191578/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc > 1:1545580/1‑71 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc > 1:1217330/1‑70 (MQ=255) aaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:2915844/63‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc < 1:3461661/71‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2568647/1‑63 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2409408/1‑63 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:2536065/4‑69 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2502871/1‑69 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa < 1:3652509/69‑1 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAc < 1:3415441/70‑1 (MQ=255) gTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTgggg < 1:120462/50‑1 (MQ=38) gTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacg < 1:2666827/71‑1 (MQ=255) tACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:3269632/41‑1 (MQ=255) tACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGt < 1:225416/71‑1 (MQ=255) tACCGGAATCTGCCGACTGGTATAACTCCAGCTACTTCATCGCCTGGGGGTCTAACGTACCGCAGAcac < 1:228291/69‑3 (MQ=255) tACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacg < 1:1049604/70‑1 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTAGATCATCGCCTGGGGGTCTAACGTACCGCAGACACGt < 1:1323966/70‑1 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTa > 1:2342308/1‑71 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTa > 1:2193418/1‑71 (MQ=255) ccGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa < 1:3919279/64‑1 (MQ=255) ccGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacgaa > 1:1522485/1‑68 (MQ=255) cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt > 1:161398/1‑48 (MQ=255) cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcc < 1:2679312/71‑1 (MQ=255) cGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAca > 1:1701875/1‑64 (MQ=255) ggAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAccc > 1:3743391/1‑71 (MQ=255) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAcgcc < 1:390411/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGTTCAAACGTGCCGCAGACGCGTACCCCg < 1:485721/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCg < 1:3692813/71‑1 (MQ=255) aaTCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:2412859/1‑60 (MQ=255) aactgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:211184/55‑1 (MQ=255) tCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTct < 1:188414/45‑2 (MQ=38) tgccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGacg > 1:170482/1‑69 (MQ=255) gccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGc > 1:1510936/4‑71 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg < 1:887414/68‑1 (MQ=255) cTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAc < 1:971018/70‑1 (MQ=255) tGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:2318042/36‑1 (MQ=255) tGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt > 1:2942217/1‑71 (MQ=255) ggTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:2889815/35‑1 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGCCACGTACGCCGGACGCCCActtct > 1:2614210/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtct > 1:2291785/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACACGTACCCCGGATGCTCActtct < 1:1890624/71‑1 (MQ=255) gTACAACTCCAGCTAAATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc < 1:3567884/70‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:1598700/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCAGGATGATCActtctt > 1:3898303/1‑70 (MQ=255) cAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCttt < 1:599472/70‑1 (MQ=255) | CGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTT > minE/832208‑832346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |