| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,307 | A→T | 38.8% | S255S (TCA→TCT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,307 | 0 | A | T | 38.8% | 67.3 / 98.5 | 85 | S255S (TCA→TCT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/33); new base T (21/12); total (40/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
GGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTG > minE/832238‑832377 | gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3053453/70‑1 (MQ=255)gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3753141/70‑1 (MQ=255) cGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg < 1:259794/71‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACg > 1:98572/1‑70 (MQ=255) gctaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:1355083/68‑1 (MQ=255) gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:2662689/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:1191578/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc < 1:618696/71‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc > 1:1545580/1‑71 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc > 1:1217330/1‑70 (MQ=255) aaaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:2915844/63‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc < 1:3461661/71‑1 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2568647/1‑63 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC‑ACATCATCGCCTGGGGGTCAAAc > 1:2409408/1‑63 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:2536065/4‑69 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2502871/1‑69 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa < 1:3652509/69‑1 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAc < 1:3415441/70‑1 (MQ=255) gTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacg < 1:2666827/71‑1 (MQ=255) tACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGt < 1:225416/71‑1 (MQ=255) tACCGGAATCTGCCGACTGGTATAACTCCAGCTACTTCATCGCCTGGGGGTCTAACGTACCGCAGAcac < 1:228291/69‑3 (MQ=255) tACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacg < 1:1049604/70‑1 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTAGATCATCGCCTGGGGGTCTAACGTACCGCAGACACGt < 1:1323966/70‑1 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTa > 1:2193418/1‑71 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTa > 1:2342308/1‑71 (MQ=255) ccGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa < 1:3919279/64‑1 (MQ=255) ccGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAcacgaa > 1:1522485/1‑68 (MQ=255) cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcc < 1:2679312/71‑1 (MQ=255) cGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAca > 1:1701875/1‑64 (MQ=255) ggAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAccc > 1:3743391/1‑71 (MQ=255) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAcgcc < 1:390411/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGTTCAAACGTGCCGCAGACGCGTACCCCg < 1:485721/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCg < 1:3692813/71‑1 (MQ=255) aaTCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:2412859/1‑60 (MQ=255) aactgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:211184/55‑1 (MQ=255) tgccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGacg > 1:170482/1‑69 (MQ=255) gccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGc > 1:1510936/4‑71 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg < 1:887414/68‑1 (MQ=255) cTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAc < 1:971018/70‑1 (MQ=255) tGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:2318042/36‑1 (MQ=255) tGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt > 1:2942217/1‑71 (MQ=255) ggTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:2889815/35‑1 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGCCACGTACGCCGGACGCCCActtct > 1:2614210/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtct > 1:2291785/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACACGTACCCCGGATGCTCActtct < 1:1890624/71‑1 (MQ=255) gTACAACTCCAGCTAAATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc < 1:3567884/70‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:1598700/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCAGGATGATCActtctt > 1:3898303/1‑70 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAg < 1:1068020/42‑1 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:2280594/69‑1 (MQ=255) cAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCttt < 1:599472/70‑1 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:1188893/1‑50 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAca > 1:1200998/1‑43 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAca > 1:2186408/1‑43 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAc > 1:637283/1‑43 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAc > 1:641960/1‑43 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAc > 1:3615971/1‑43 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAcc > 1:2859635/1‑70 (MQ=255) aCTCCAGCTACATAATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAc > 1:1291364/1‑70 (MQ=255) gtCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtc < 1:1262830/63‑1 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTg > 1:900394/1‑70 (MQ=255) ccAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:1608210/1‑35 (MQ=38) ccAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc > 1:1116364/1‑36 (MQ=255) aGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGt > 1:459677/1‑71 (MQ=255) cTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAct < 1:463447/56‑1 (MQ=255) cTACATCATCGCCTGGGGGTAAAACGTGCCGCAGACGCGTACCCCGGa > 1:3713743/1‑48 (MQ=255) aCATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:2290592/58‑1 (MQ=255) catcatCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt < 1:866089/70‑1 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAc > 1:954430/1‑51 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTa < 1:1878824/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:714420/56‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:3473666/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:2476045/71‑1 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActt < 1:277374/51‑1 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:3042733/70‑1 (MQ=255) cGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg > 1:1611567/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg > 1:2569466/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:84732/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:531809/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:3844702/71‑1 (MQ=255) ccTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAggg < 1:2463365/71‑1 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3366837/1‑71 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3325524/1‑71 (MQ=255) tGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAc > 1:467969/1‑71 (MQ=255) gggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTA‑AAAGGGAcc > 1:3904245/1‑70 (MQ=255) ggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa < 1:134540/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:353513/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa > 1:317598/1‑70 (MQ=255) gTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAgg < 1:3732973/63‑1 (MQ=255) gTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaaa < 1:759853/71‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGa < 1:1595823/45‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:838937/1‑71 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3450809/1‑71 (MQ=255) | GGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTG > minE/832238‑832377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |