| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,334 | T→C | 21.4% | D264D (GAT→GAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,334 | 0 | T | C | 21.4% | 137.2 / 32.9 | 70 | D264D (GAT→GAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/34); new base C (8/7); total (29/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAA > minE/832266‑832403 | gccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGc > 1:1510936/4‑71 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg < 1:887414/68‑1 (MQ=255) cTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAc < 1:971018/70‑1 (MQ=255) tGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt > 1:2942217/1‑71 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGCCACGTACGCCGGACGCCCActtct > 1:2614210/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtct > 1:2291785/1‑71 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACACGTACCCCGGATGCTCActtct < 1:1890624/71‑1 (MQ=255) gTACAACTCCAGCTAAATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc < 1:3567884/70‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:1598700/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCAGGATGATCActtctt > 1:3898303/1‑70 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:2280594/69‑1 (MQ=255) cAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCttt < 1:599472/70‑1 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAcc > 1:2859635/1‑70 (MQ=255) aCTCCAGCTACATAATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAc > 1:1291364/1‑70 (MQ=255) gtCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtc < 1:1262830/63‑1 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTg > 1:900394/1‑70 (MQ=255) aGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGt > 1:459677/1‑71 (MQ=255) cTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAct < 1:463447/56‑1 (MQ=255) aCATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:2290592/58‑1 (MQ=255) catcatCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt < 1:866089/70‑1 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAc > 1:954430/1‑51 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTa < 1:1878824/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:714420/56‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:3473666/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:2476045/71‑1 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActt < 1:277374/51‑1 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:3042733/70‑1 (MQ=255) cGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg > 1:1611567/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:84732/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:3844702/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:531809/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg > 1:2569466/1‑71 (MQ=255) ccTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAggg < 1:2463365/71‑1 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3325524/1‑71 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3366837/1‑71 (MQ=255) tGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAc > 1:467969/1‑71 (MQ=255) gggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTA‑AAAGGGAcc > 1:3904245/1‑70 (MQ=255) ggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa < 1:134540/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:353513/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa > 1:317598/1‑70 (MQ=255) gTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAgg < 1:3732973/63‑1 (MQ=255) gTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaaa < 1:759853/71‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGa < 1:1595823/45‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3450809/1‑71 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:838937/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTa > 1:2319136/1‑37 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGCACCAAAACTGTTGcc < 1:1592419/71‑1 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg > 1:2477889/1‑70 (MQ=255) ccGCAGACACGTACGCCGGACGCCCACTTCTTTAc < 1:1460777/35‑1 (MQ=19) cAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca > 1:1664343/1‑70 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:108177/71‑1 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:3465792/71‑1 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac > 1:3088918/1‑68 (MQ=255) cgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGa < 1:479214/71‑1 (MQ=255) gTACGCCGGACGCCCACTTCTTTACCGAACTACGCTACAAAGGCACAAAAAcc > 1:1529866/1‑52 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAggg < 1:3381511/44‑1 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGa > 1:591485/1‑45 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGa > 1:1628907/1‑45 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAcc < 1:2224803/47‑1 (MQ=255) tACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:420838/39‑1 (MQ=255) tACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACt < 1:217214/71‑1 (MQ=255) aCCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa > 1:2124047/1‑38 (MQ=255) aCCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGa > 1:894911/1‑68 (MQ=255) ccccGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:3627714/36‑1 (MQ=255) cccGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:3251747/36‑1 (MQ=255) cGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGt > 1:1109946/1‑49 (MQ=255) cGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCt < 1:223840/71‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAcc < 1:3524582/40‑1 (MQ=255) aTGCTCACTTCTTTACTTAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa < 1:2710426/71‑1 (MQ=255) aTGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGa < 1:1685507/70‑1 (MQ=255) | GCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAA > minE/832266‑832403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |