| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,582 | T→A | 9.6% | F360Y (TTC→TAC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,582 | 0 | T | A | 9.6% | 137.8 / 7.0 | 52 | F360Y (TTC→TAC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (21/26); new base A (3/2); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
ACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGC > minE/189512‑189649 | acCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCtt < 1:507438/71‑1 (MQ=255) cATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:779238/71‑1 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaa < 1:3336562/71‑1 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa > 1:367910/1‑70 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa > 1:3424102/1‑70 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa > 1:1892658/1‑70 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACg > 1:2187315/1‑71 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTCGTATGAGCGGCGAGCGTGATGCGGGAAAAGTGATCTTCGaa > 1:2855256/1‑68 (MQ=255) tCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAAc < 1:3640658/66‑1 (MQ=255) cGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:1163969/1‑70 (MQ=255) gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGaaa < 1:573051/62‑1 (MQ=255) gATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:1372304/1‑71 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:1228178/1‑69 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc > 1:895463/1‑71 (MQ=255) tCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc > 1:1263497/1‑71 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:1081263/60‑1 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCa < 1:1621085/60‑1 (MQ=255) ggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt > 1:1494879/1‑71 (MQ=255) gTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCa < 1:3047840/55‑1 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATg > 1:2345305/1‑71 (MQ=255) tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:2213161/48‑1 (MQ=255) gTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTggcg < 1:1708418/71‑1 (MQ=255) tGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGttc > 1:2339175/1‑70 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAAt > 1:3239481/1‑45 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt < 1:2899027/70‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt < 1:1459859/70‑1 (MQ=255) aGCGGCGAGCTTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATc > 1:31746/1‑71 (MQ=255) gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCgt < 1:2919381/45‑2 (MQ=255) gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1353903/71‑1 (MQ=255) ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:3767439/71‑1 (MQ=255) ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:3518502/71‑1 (MQ=255) ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:168812/71‑1 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGCCGGCGTTATCGCa < 1:2304605/70‑1 (MQ=255) gAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:2937092/47‑1 (MQ=255) cGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc > 1:602715/1‑71 (MQ=255) gtTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCtt < 1:3125983/67‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCTACAAAATGCTGGCGGCGTTATCGCAGGCt < 1:598359/65‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt > 1:2457588/1‑70 (MQ=255) tGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:555725/70‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGc < 1:702812/41‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATc > 1:875236/1‑70 (MQ=255) gggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:3431554/4‑71 (MQ=255) gggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:1732390/4‑71 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcac < 1:1232237/71‑1 (MQ=255) aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcaca < 1:2900179/71‑1 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAAt > 1:215213/1‑35 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAAt > 1:3676241/1‑35 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2430194/51‑1 (MQ=255) aaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:3542458/1‑70 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1927645/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa > 1:2282388/1‑70 (MQ=255) aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa < 1:3768447/69‑1 (MQ=255) aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:238895/67‑1 (MQ=255) ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2168222/54‑1 (MQ=255) ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:2955028/1‑68 (MQ=255) ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc > 1:754662/1‑69 (MQ=255) | ACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGC > minE/189512‑189649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |