| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,355 | G→A | 6.9% | V271V (GTG→GTA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,355 | 0 | G | A | 6.9% | 221.8 / 3.3 | 72 | V271V (GTG→GTA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (31/36); new base A (3/2); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCT > minE/832289‑832423 | catcatCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt < 1:866089/70‑1 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTa < 1:1878824/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:3473666/71‑1 (MQ=255) atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTa < 1:2476045/71‑1 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:3042733/70‑1 (MQ=255) cGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg > 1:1611567/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:3844702/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:531809/71‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg > 1:2569466/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:84732/71‑1 (MQ=255) ccTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAggg < 1:2463365/71‑1 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3325524/1‑71 (MQ=255) tGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCAc > 1:3366837/1‑71 (MQ=255) tGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAc > 1:467969/1‑71 (MQ=255) gggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTA‑AAAGGGAcc > 1:3904245/1‑70 (MQ=255) ggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa < 1:134540/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:353513/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCa > 1:317598/1‑70 (MQ=255) gTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAgg < 1:3732973/63‑1 (MQ=255) gTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaaa < 1:759853/71‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3450809/1‑71 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:838937/1‑71 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGCACCAAAACTGTTGcc < 1:1592419/71‑1 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg > 1:2477889/1‑70 (MQ=255) cAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca > 1:1664343/1‑70 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:3465792/71‑1 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:108177/71‑1 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac > 1:3088918/1‑68 (MQ=255) cgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGa < 1:479214/71‑1 (MQ=255) gTACGCCGGACGCCCACTTCTTTACCGAACTACGCTACAAAGGCACAAAAAcc > 1:1529866/1‑52 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAggg < 1:3381511/44‑1 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGa > 1:591485/1‑45 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGa > 1:1628907/1‑45 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAcc < 1:2224803/47‑1 (MQ=255) tACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:420838/39‑1 (MQ=255) tACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACt < 1:217214/71‑1 (MQ=255) aCCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa > 1:2124047/1‑38 (MQ=255) aCCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGa > 1:894911/1‑68 (MQ=255) ccccGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:3627714/36‑1 (MQ=255) cccGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:3251747/36‑1 (MQ=255) cGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGt > 1:1109946/1‑49 (MQ=255) cGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCt < 1:223840/71‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGAcc < 1:3524582/40‑1 (MQ=255) aTGCTCACTTCTTTACTTAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa < 1:2710426/71‑1 (MQ=255) aTGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGa < 1:1685507/70‑1 (MQ=255) cTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:2569078/70‑1 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:1687722/36‑1 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGc > 1:2013815/1‑63 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGc > 1:1877977/1‑63 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATc > 1:2709380/1‑70 (MQ=255) cACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa > 1:2469899/1‑66 (MQ=255) cACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa > 1:1332984/1‑66 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAAt < 1:1206059/67‑1 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:945253/1‑70 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:975520/1‑70 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:280352/1‑70 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:1840944/1‑70 (MQ=255) cttcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:3150950/71‑1 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa > 1:3131431/1‑70 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:493160/1‑71 (MQ=255) tttACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac < 1:2742556/46‑1 (MQ=255) tttACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac < 1:2584679/46‑1 (MQ=255) tttACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa < 1:2380354/68‑1 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac > 1:1450416/1‑43 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:357423/70‑1 (MQ=255) cTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGc < 1:2708205/71‑1 (MQ=255) tGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTcac > 1:851099/1‑39 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCg > 1:3872703/1‑57 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCg > 1:576091/1‑57 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCg > 1:1129232/1‑57 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:2276500/69‑1 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCt < 1:219463/70‑1 (MQ=255) | CATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCT > minE/832289‑832423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |