| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,423 | T→A | 12.2% | L294Q (CTG→CAG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,423 | 0 | T | A | 12.2% | 241.1 / 22.9 | 90 | L294Q (CTG→CAG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (41/38); new base A (5/6); total (46/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGT > minE/832354‑832490 | tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCt < 1:219463/70‑1 (MQ=255) cGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtgt < 1:1111121/70‑1 (MQ=255) gTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtgg < 1:2952137/71‑1 (MQ=255) gTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtgg < 1:3665311/71‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc < 1:1655177/71‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc < 1:3627765/71‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc < 1:2074091/71‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc < 1:884587/71‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctg > 1:2784215/1‑71 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctg > 1:278699/1‑71 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctg > 1:456901/1‑71 (MQ=255) aaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctg < 1:3156765/68‑1 (MQ=255) gggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc < 1:125354/71‑1 (MQ=255) gggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc < 1:1716563/71‑1 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCg > 1:106184/1‑70 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtgtg < 1:2162351/59‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:829271/71‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:2526951/71‑1 (MQ=255) cAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:3288342/62‑1 (MQ=255) aaaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctggc > 1:934550/1‑62 (MQ=255) aaaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:1192691/71‑1 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCa > 1:2768099/1‑62 (MQ=255) aaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg > 1:1602109/1‑71 (MQ=255) aaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg > 1:2216480/1‑71 (MQ=255) aCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtgt > 1:3613883/1‑52 (MQ=255) aCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtgt > 1:930493/1‑52 (MQ=255) tGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCa > 1:2970708/1‑71 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc > 1:3620646/1‑71 (MQ=255) gCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAg > 1:2231822/1‑63 (MQ=255) cGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGAt < 1:350066/70‑1 (MQ=255) cGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATg < 1:708412/71‑1 (MQ=255) gTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGc < 1:1945686/71‑1 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:2179610/53‑1 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGc > 1:1565246/1‑68 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:1494829/1‑70 (MQ=255) ccAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAc > 1:3241691/1‑46 (MQ=255) cAGACTACGCTGCAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAGACAGGGCACCGATGCGGCAATg > 1:1818749/1‑71 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg < 1:2466817/71‑1 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:384313/1‑71 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:1469432/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2809393/71‑1 (MQ=255) gACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:3039408/1‑70 (MQ=255) actactCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCa < 1:3502691/54‑1 (MQ=19) actactCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCa < 1:816732/54‑1 (MQ=19) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:2164379/1‑68 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:3805538/1‑68 (MQ=255) tACGCTGAAATCGCCAAACTGTGAGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:1175749/1‑68 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:2326459/69‑1 (MQ=255) gCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc < 1:1074059/71‑1 (MQ=255) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCa > 1:3584605/1‑47 (MQ=19) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc > 1:3692799/1‑46 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCa > 1:1536119/1‑47 (MQ=255) aaaTCGCCTAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:1715310/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAc > 1:1329575/1‑46 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAc > 1:2850585/1‑46 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:3213067/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2973687/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:966293/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:1942153/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2522704/70‑1 (MQ=255) aaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATgg > 1:1098882/1‑71 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg > 1:2943493/1‑71 (MQ=255) tCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGc < 1:3717259/71‑1 (MQ=255) cGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg > 1:1321583/1‑62 (MQ=255) gCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGcc < 1:1701434/70‑1 (MQ=255) gCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCa > 1:3053525/1‑71 (MQ=255) ccAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCat < 1:418280/71‑2 (MQ=255) cAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCActg < 1:1583995/41‑3 (MQ=19) cAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCatg > 1:2124485/1‑69 (MQ=255) aaaTTGTGCGACCAGTGGCTGGCACCGAAACAagg < 1:2224396/35‑1 (MQ=16) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCa > 1:1999934/1‑37 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:3310625/71‑1 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:1055514/71‑1 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:3767962/1‑45 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCAcct > 1:1642185/1‑68 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACg > 1:1401032/1‑69 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:1703189/1‑70 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:3090243/1‑70 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:1569310/1‑70 (MQ=255) tgtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2401328/48‑1 (MQ=255) tgtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2405978/48‑1 (MQ=255) tgtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAtt > 1:2941500/1‑70 (MQ=255) gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgc > 1:1987441/1‑71 (MQ=255) gtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGc > 1:2269038/1‑61 (MQ=255) tgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAttt > 1:596674/1‑68 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc < 1:3913534/71‑1 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc < 1:2682659/71‑1 (MQ=255) gATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:1933014/43‑1 (MQ=255) aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:3609478/1‑71 (MQ=255) aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:3799497/1‑71 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:1039792/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:3653872/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:3353623/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg > 1:2492217/1‑69 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:1233680/1‑70 (MQ=255) tgtgGCTGGCACCGAAACATGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAt > 1:2915741/1‑61 (MQ=255) | TGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGT > minE/832354‑832490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |