| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,464 | C→A | 7.2% | L308M (CTG→ATG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,464 | 0 | C | A | 7.2% | 267.9 / 4.0 | 82 | L308M (CTG→ATG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (40/36); new base A (4/2); total (44/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
CGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGT > minE/832397‑832534 | cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:2326459/69‑1 (MQ=255) gCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc < 1:1074059/71‑1 (MQ=255) aaaTCGCCTAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:1715310/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:966293/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2522704/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2973687/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:1942153/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:3213067/70‑1 (MQ=255) aaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATgg > 1:1098882/1‑71 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg > 1:2943493/1‑71 (MQ=255) tCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGc < 1:3717259/71‑1 (MQ=255) cGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg > 1:1321583/1‑62 (MQ=255) gCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGcc < 1:1701434/70‑1 (MQ=255) gCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCa > 1:3053525/1‑71 (MQ=255) ccAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCat < 1:418280/71‑2 (MQ=255) cAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCatg > 1:2124485/1‑69 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:3310625/71‑1 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:1055514/71‑1 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCAcct > 1:1642185/1‑68 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACg > 1:1401032/1‑69 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:1703189/1‑70 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:3090243/1‑70 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt > 1:1569310/1‑70 (MQ=255) tgtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAtt > 1:2941500/1‑70 (MQ=255) gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgc > 1:1987441/1‑71 (MQ=255) gtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGc > 1:2269038/1‑61 (MQ=255) tgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAttt > 1:596674/1‑68 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc < 1:2682659/71‑1 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc < 1:3913534/71‑1 (MQ=255) aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:3799497/1‑71 (MQ=255) aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:3609478/1‑71 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:1039792/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:3353623/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:3653872/45‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg > 1:2492217/1‑69 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:1233680/1‑70 (MQ=255) tgtgGCTGGCACCGAAACATGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAt > 1:2915741/1‑61 (MQ=255) tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg < 1:2416428/65‑1 (MQ=255) tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGa < 1:986865/68‑1 (MQ=255) tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGACACGTAATGCTGCGTGa < 1:2115255/68‑1 (MQ=255) tggctgacACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAAtt > 1:802/1‑71 (MQ=255) ggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTc > 1:2049401/1‑71 (MQ=255) tggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCAc > 1:2446409/1‑71 (MQ=255) gcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt > 1:765298/1‑71 (MQ=255) gcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt < 1:2313349/71‑1 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa < 1:2477854/37‑1 (MQ=255) gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACa < 1:843796/71‑1 (MQ=255) gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACa < 1:2194359/71‑1 (MQ=255) aaaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACaa > 1:1302991/1‑71 (MQ=255) aaaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACaa > 1:1622538/1‑71 (MQ=255) aaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt > 1:2583947/1‑64 (MQ=255) cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAAccc < 1:3215379/71‑1 (MQ=255) cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAAcc > 1:2465597/1‑70 (MQ=255) gggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCaa < 1:918481/71‑1 (MQ=255) ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:1619693/1‑71 (MQ=255) ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:1755151/1‑71 (MQ=255) ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:1667857/1‑71 (MQ=255) ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:2512421/1‑71 (MQ=255) ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:2616635/1‑71 (MQ=255) gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:1785149/1‑71 (MQ=255) gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:3395877/1‑71 (MQ=255) cACCGATGCGGCAATGGCGCTTGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGcc < 1:381264/71‑1 (MQ=255) cACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg < 1:2637359/69‑1 (MQ=255) cACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGcc < 1:1731723/71‑1 (MQ=255) ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg > 1:313832/1‑71 (MQ=255) ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg > 1:2663934/1‑71 (MQ=255) ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg > 1:3907090/1‑71 (MQ=255) cGATGCGGCAATGGCGCTGGCGATGGGCCACGTaa > 1:1561248/1‑35 (MQ=255) cGATGCGGCAATGGCGCTGGCGATGGGCCACGTaa > 1:350319/1‑35 (MQ=255) aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGa > 1:347961/1‑55 (MQ=255) aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGa > 1:93241/1‑55 (MQ=255) aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt < 1:2622479/71‑1 (MQ=255) aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt < 1:2626359/71‑1 (MQ=255) tGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAtt < 1:1023727/71‑1 (MQ=255) gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTc < 1:182222/51‑1 (MQ=255) gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc < 1:2418178/54‑1 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt < 1:1676676/70‑1 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt < 1:1953701/70‑1 (MQ=255) ggCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt < 1:547279/69‑1 (MQ=255) tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:535717/1‑71 (MQ=255) tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:317888/1‑71 (MQ=255) tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:1980104/1‑71 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:358105/1‑53 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg > 1:1924440/1‑53 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACt < 1:530625/70‑1 (MQ=255) cgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAt < 1:1191480/71‑1 (MQ=255) gcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAt < 1:717870/70‑1 (MQ=255) cTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtgt < 1:2270230/71‑1 (MQ=255) cTGGCGATGGGCCACGTAATGATGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtgt > 1:245330/1‑71 (MQ=255) | CGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGT > minE/832397‑832534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |