Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,464 C→A 7.2% L308M (CTG→ATG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,4640CA 7.2% 267.9 / 4.0 82L308M (CTG→ATG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (40/36);  new base A (4/2);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

CGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGT  >  minE/832397‑832534
                                                                   |                                                                      
cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt                                                                       <  1:2326459/69‑1 (MQ=255)
 gCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc                                                                    <  1:1074059/71‑1 (MQ=255)
     aaaTCGCCTAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:1715310/70‑1 (MQ=255)
     aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:966293/70‑1 (MQ=255)
     aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:2522704/70‑1 (MQ=255)
     aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:2973687/70‑1 (MQ=255)
     aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:1942153/70‑1 (MQ=255)
     aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt                                                                 <  1:3213067/70‑1 (MQ=255)
      aaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATgg                                                               >  1:1098882/1‑71 (MQ=255)
       aTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                                                              >  1:2943493/1‑71 (MQ=255)
        tCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGc                                                             <  1:3717259/71‑1 (MQ=255)
         cGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg                                                                     >  1:1321583/1‑62 (MQ=255)
          gCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGcc                                                            <  1:1701434/70‑1 (MQ=255)
          gCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCa                                                           >  1:3053525/1‑71 (MQ=255)
           ccAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCat                                                          <  1:418280/71‑2 (MQ=255)
            cAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCatg                                                         >  1:2124485/1‑69 (MQ=255)
             aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt                                                        <  1:3310625/71‑1 (MQ=255)
             aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt                                                        <  1:1055514/71‑1 (MQ=255)
              aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCAcct                                                        >  1:1642185/1‑68 (MQ=255)
              aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACg                                                         >  1:1401032/1‑69 (MQ=255)
              aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt                                                        >  1:1703189/1‑70 (MQ=255)
              aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt                                                        >  1:3090243/1‑70 (MQ=255)
              aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt                                                        >  1:1569310/1‑70 (MQ=255)
                 tgtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAtt                                                    >  1:2941500/1‑70 (MQ=255)
                  gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgc                                                   >  1:1987441/1‑71 (MQ=255)
                  gtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGc                                                             >  1:2269038/1‑61 (MQ=255)
                   tgCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGTGAttt                                                   >  1:596674/1‑68 (MQ=255)
                     cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc                                                <  1:2682659/71‑1 (MQ=255)
                     cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgctgc                                                <  1:3913534/71‑1 (MQ=255)
                       aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt                                              >  1:3799497/1‑71 (MQ=255)
                       aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt                                              >  1:3609478/1‑71 (MQ=255)
                        tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt                                                                       <  1:1039792/45‑1 (MQ=255)
                        tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt                                                                       <  1:3353623/45‑1 (MQ=255)
                        tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt                                                                       <  1:3653872/45‑1 (MQ=255)
                        tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg                                               >  1:2492217/1‑69 (MQ=255)
                        tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt                                              >  1:1233680/1‑70 (MQ=255)
                          tgtgGCTGGCACCGAAACATGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAt                                                     >  1:2915741/1‑61 (MQ=255)
                            tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg                                               <  1:2416428/65‑1 (MQ=255)
                            tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGa                                            <  1:986865/68‑1 (MQ=255)
                            tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGACACGTAATGCTGCGTGa                                            <  1:2115255/68‑1 (MQ=255)
                            tggctgacACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAAtt                                         >  1:802/1‑71 (MQ=255)
                             ggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTc                                        >  1:2049401/1‑71 (MQ=255)
                                tggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCAc                                     >  1:2446409/1‑71 (MQ=255)
                                  gcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt                                   >  1:765298/1‑71 (MQ=255)
                                  gcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt                                   <  1:2313349/71‑1 (MQ=255)
                                     ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa                                                                  <  1:2477854/37‑1 (MQ=255)
                                       gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACa                              <  1:843796/71‑1 (MQ=255)
                                       gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACa                              <  1:2194359/71‑1 (MQ=255)
                                        aaaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACaa                             >  1:1302991/1‑71 (MQ=255)
                                        aaaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACaa                             >  1:1622538/1‑71 (MQ=255)
                                         aaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCt                                   >  1:2583947/1‑64 (MQ=255)
                                           cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAAccc                          <  1:3215379/71‑1 (MQ=255)
                                           cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAAcc                           >  1:2465597/1‑70 (MQ=255)
                                             gggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCaa                        <  1:918481/71‑1 (MQ=255)
                                              ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:1619693/1‑71 (MQ=255)
                                              ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:1755151/1‑71 (MQ=255)
                                              ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:1667857/1‑71 (MQ=255)
                                              ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:2512421/1‑71 (MQ=255)
                                              ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:2616635/1‑71 (MQ=255)
                                               gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc                      >  1:1785149/1‑71 (MQ=255)
                                               gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc                      >  1:3395877/1‑71 (MQ=255)
                                                cACCGATGCGGCAATGGCGCTTGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGcc                     <  1:381264/71‑1 (MQ=255)
                                                cACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       <  1:2637359/69‑1 (MQ=255)
                                                cACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGcc                     <  1:1731723/71‑1 (MQ=255)
                                                  ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg                   >  1:313832/1‑71 (MQ=255)
                                                  ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg                   >  1:2663934/1‑71 (MQ=255)
                                                  ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAg                   >  1:3907090/1‑71 (MQ=255)
                                                   cGATGCGGCAATGGCGCTGGCGATGGGCCACGTaa                                                      >  1:1561248/1‑35 (MQ=255)
                                                   cGATGCGGCAATGGCGCTGGCGATGGGCCACGTaa                                                      >  1:350319/1‑35 (MQ=255)
                                                     aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGa                                >  1:347961/1‑55 (MQ=255)
                                                     aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGa                                >  1:93241/1‑55 (MQ=255)
                                                     aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt                <  1:2622479/71‑1 (MQ=255)
                                                     aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt                <  1:2626359/71‑1 (MQ=255)
                                                      tGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAtt               <  1:1023727/71‑1 (MQ=255)
                                                       gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTc                                  <  1:182222/51‑1 (MQ=255)
                                                       gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc                               <  1:2418178/54‑1 (MQ=255)
                                                        cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt              <  1:1676676/70‑1 (MQ=255)
                                                        cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt              <  1:1953701/70‑1 (MQ=255)
                                                         ggCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt              <  1:547279/69‑1 (MQ=255)
                                                              tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc       >  1:535717/1‑71 (MQ=255)
                                                              tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc       >  1:317888/1‑71 (MQ=255)
                                                              tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc       >  1:1980104/1‑71 (MQ=255)
                                                                gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:358105/1‑53 (MQ=255)
                                                                gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAg                       >  1:1924440/1‑53 (MQ=255)
                                                                gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACt      <  1:530625/70‑1 (MQ=255)
                                                                 cgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAt    <  1:1191480/71‑1 (MQ=255)
                                                                  gcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAt    <  1:717870/70‑1 (MQ=255)
                                                                   cTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtgt  <  1:2270230/71‑1 (MQ=255)
                                                                   cTGGCGATGGGCCACGTAATGATGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtgt  >  1:245330/1‑71 (MQ=255)
                                                                   |                                                                      
CGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGT  >  minE/832397‑832534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: