| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,712 | T→G | 8.7% | S390S (TCT→TCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,712 | 0 | T | G | 8.7% | 109.8 / 4.5 | 46 | S390S (TCT→TCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/20); new base G (2/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCGAAGA > minE/832644‑832780 | gAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAt > 1:3845632/1‑71 (MQ=255)agcaaCAATCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGAt < 1:1198656/68‑1 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTg > 1:1945875/1‑71 (MQ=255) aaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTgg < 1:1870889/71‑1 (MQ=255) aCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGc > 1:690940/1‑71 (MQ=255) aaTCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCtt < 1:939188/71‑1 (MQ=255) aaTCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCt < 1:694246/70‑1 (MQ=255) aaTCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGttt > 1:63254/1‑71 (MQ=255) ccGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCg > 1:398101/1‑71 (MQ=255) gAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg < 1:3497072/71‑1 (MQ=255) gAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg > 1:3505311/1‑71 (MQ=255) gAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg < 1:320852/71‑1 (MQ=255) gAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg > 1:64398/1‑71 (MQ=255) gtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTggg < 1:699230/70‑1 (MQ=255) tGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:2806182/54‑1 (MQ=255) tGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:670058/54‑1 (MQ=255) aaaaCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg > 1:2195524/1‑65 (MQ=255) aaaCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTg < 1:3455641/64‑1 (MQ=255) aaCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCgaga < 1:3220860/71‑1 (MQ=255) aCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGaa < 1:308044/71‑1 (MQ=255) cTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAAt > 1:1104260/1‑71 (MQ=255) tttAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAggg > 1:3295014/1‑65 (MQ=255) tttAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAgg > 1:959610/1‑64 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATgg < 1:1235800/70‑1 (MQ=255) aaTACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGaa > 1:783362/1‑71 (MQ=255) tACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg > 1:3758320/1‑50 (MQ=255) aCCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGCCTTCCGCTGGGGCGAGAAGGGCa < 1:757441/61‑1 (MQ=255) tGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg < 1:3951377/44‑1 (MQ=255) tGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGagc < 1:558955/71‑1 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGTCAAATGGAATCTTGagcag > 1:3361745/1‑71 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGagcag > 1:3840418/1‑71 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGagcag > 1:1921927/1‑71 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGagca < 1:683252/70‑1 (MQ=255) gAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCa < 1:1670657/52‑1 (MQ=255) gAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg < 1:3736617/64‑1 (MQ=255) aaTGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAgcgc > 1:431527/1‑70 (MQ=255) tGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGAc > 1:2860741/1‑71 (MQ=255) tGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGAc < 1:3678742/71‑1 (MQ=255) ggTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGa < 1:3177440/69‑1 (MQ=255) gtgcCGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGaa > 1:3104719/3‑50 (MQ=25) aaCGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTgg > 1:1963597/1‑71 (MQ=255) aaCGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTg > 1:3528698/1‑70 (MQ=255) aCGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg > 1:3896115/1‑48 (MQ=255) ggTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCga < 1:1908242/71‑1 (MQ=255) gTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCg > 1:3730915/1‑69 (MQ=255) cTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCgaaga > 1:3148223/1‑70 (MQ=255) cTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCgaaga < 1:1464670/70‑1 (MQ=255) cTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGAGCGACGGCAAAACTGGCgaaga < 1:3844998/70‑1 (MQ=255) | GAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCGAAGA > minE/832644‑832780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |