| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,144 | C→T | 8.6% | H534H (CAC→CAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,144 | 0 | C | T | 8.6% | 169.6 / 5.2 | 58 | H534H (CAC→CAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/19); new base T (3/2); total (37/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTC > minE/833079‑833214 | ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCAc < 1:627827/66‑1 (MQ=255)ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCg > 1:595709/1‑71 (MQ=255) gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc > 1:529407/1‑71 (MQ=255) gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc < 1:862890/71‑1 (MQ=255) tctcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgat > 1:1992396/1‑71 (MQ=255) cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt < 1:530504/71‑1 (MQ=255) cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt < 1:3299636/71‑1 (MQ=255) cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt < 1:1469768/71‑1 (MQ=255) aGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg > 1:872914/1‑59 (MQ=255) gATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatgat > 1:3876038/1‑63 (MQ=255) attattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCgg < 1:1306554/71‑1 (MQ=255) tattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACgg < 1:1379086/48‑1 (MQ=255) ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatga > 1:875833/1‑57 (MQ=255) ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg < 1:296428/71‑1 (MQ=255) accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTa > 1:1762745/3‑58 (MQ=255) accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAt > 1:3110448/3‑59 (MQ=255) accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCagg < 1:1490025/69‑1 (MQ=255) gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCtgg < 1:1212568/70‑1 (MQ=255) gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCggg < 1:3083936/70‑1 (MQ=255) ccATCGCCCGTGAATTTGCCGCTACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggt > 1:843456/2‑70 (MQ=255) aTCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt < 1:1204109/71‑1 (MQ=255) cGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc > 1:557628/1‑58 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa > 1:879762/1‑71 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa < 1:3474469/70‑1 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa < 1:742685/70‑1 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa < 1:3593080/70‑1 (MQ=255) gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc < 1:1534219/53‑1 (MQ=255) gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt > 1:620164/1‑52 (MQ=255) gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg > 1:718416/1‑71 (MQ=255) gAATTTGCCGATAACGCTGATAAAACGCACGGGCGTTc < 1:1627059/38‑1 (MQ=255) aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc > 1:1520512/1‑60 (MQ=255) aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg > 1:1775315/1‑70 (MQ=255) aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg > 1:3614234/1‑70 (MQ=255) aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg > 1:139358/1‑70 (MQ=255) gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa > 1:2474027/1‑71 (MQ=255) gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa > 1:147388/1‑71 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt < 1:1002676/40‑1 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt > 1:1271685/1‑70 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:1047353/1‑71 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc < 1:3332809/71‑1 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:1753874/1‑71 (MQ=255) tAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:2822664/1‑70 (MQ=255) aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa > 1:3473686/1‑71 (MQ=255) cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt > 1:1825987/1‑48 (MQ=255) cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg > 1:2847355/1‑68 (MQ=255) cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata > 1:187174/1‑71 (MQ=255) gCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt < 1:1861822/65‑1 (MQ=255) tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg > 1:1678013/1‑70 (MQ=255) aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCgggg > 1:1118939/1‑41 (MQ=255) aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc > 1:30463/1‑71 (MQ=255) aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCgggg > 1:3726107/1‑39 (MQ=255) aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa > 1:336373/1‑53 (MQ=255) aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt > 1:1893518/1‑40 (MQ=255) aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg < 1:393827/71‑1 (MQ=255) cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc > 1:2944199/1‑36 (MQ=255) cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt > 1:1435509/1‑71 (MQ=255) gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg < 1:2385400/71‑3 (MQ=255) cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa > 1:3860512/1‑50 (MQ=255) cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg > 1:2284480/1‑71 (MQ=255) cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc > 1:61076/1‑71 (MQ=255) | GGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTC > minE/833079‑833214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |