Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,144 C→T 8.6% H534H (CAC→CAT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1440CT 8.6% 169.6 / 5.2 58H534H (CAC→CATnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (34/19);  new base T (3/2);  total (37/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTC  >  minE/833079‑833214
                                                                 |                                                                      
ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCAc                                                                        <  1:627827/66‑1 (MQ=255)
ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCg                                                                   >  1:595709/1‑71 (MQ=255)
   gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc                                                                >  1:529407/1‑71 (MQ=255)
   gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc                                                                <  1:862890/71‑1 (MQ=255)
      tctcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgat                                                             >  1:1992396/1‑71 (MQ=255)
               cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                    <  1:530504/71‑1 (MQ=255)
               cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                    <  1:3299636/71‑1 (MQ=255)
               cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                    <  1:1469768/71‑1 (MQ=255)
                aGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg                                                               >  1:872914/1‑59 (MQ=255)
                 gATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatgat                                                          >  1:3876038/1‑63 (MQ=255)
                  attattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCgg                                                 <  1:1306554/71‑1 (MQ=255)
                    tattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACgg                                                                      <  1:1379086/48‑1 (MQ=255)
                      ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatga                                                           >  1:875833/1‑57 (MQ=255)
                      ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg                                             <  1:296428/71‑1 (MQ=255)
                        accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTa                                                        >  1:1762745/3‑58 (MQ=255)
                        accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAt                                                       >  1:3110448/3‑59 (MQ=255)
                        accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCagg                                           <  1:1490025/69‑1 (MQ=255)
                         gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCtgg                                           <  1:1212568/70‑1 (MQ=255)
                         gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCggg                                           <  1:3083936/70‑1 (MQ=255)
                         ccATCGCCCGTGAATTTGCCGCTACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggt                                          >  1:843456/2‑70 (MQ=255)
                           aTCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                        <  1:1204109/71‑1 (MQ=255)
                             cGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc                                                   >  1:557628/1‑58 (MQ=255)
                              gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                     >  1:879762/1‑71 (MQ=255)
                              gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                      <  1:3474469/70‑1 (MQ=255)
                              gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                      <  1:742685/70‑1 (MQ=255)
                              gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                      <  1:3593080/70‑1 (MQ=255)
                                  gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc                                                   <  1:1534219/53‑1 (MQ=255)
                                  gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                    >  1:620164/1‑52 (MQ=255)
                                    gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg                               >  1:718416/1‑71 (MQ=255)
                                    gAATTTGCCGATAACGCTGATAAAACGCACGGGCGTTc                                                                <  1:1627059/38‑1 (MQ=255)
                                     aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                         >  1:1520512/1‑60 (MQ=255)
                                      aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                              >  1:1775315/1‑70 (MQ=255)
                                      aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                              >  1:3614234/1‑70 (MQ=255)
                                      aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                              >  1:139358/1‑70 (MQ=255)
                                          gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                         >  1:2474027/1‑71 (MQ=255)
                                          gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                         >  1:147388/1‑71 (MQ=255)
                                              aTAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                    <  1:1002676/40‑1 (MQ=255)
                                              aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                      >  1:1271685/1‑70 (MQ=255)
                                              aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                     >  1:1047353/1‑71 (MQ=255)
                                              aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                     <  1:3332809/71‑1 (MQ=255)
                                              aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                     >  1:1753874/1‑71 (MQ=255)
                                               tAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                     >  1:2822664/1‑70 (MQ=255)
                                                aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                   >  1:3473686/1‑71 (MQ=255)
                                                  cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                        >  1:1825987/1‑48 (MQ=255)
                                                  cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg                    >  1:2847355/1‑68 (MQ=255)
                                                  cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata                 >  1:187174/1‑71 (MQ=255)
                                                   gCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                      <  1:1861822/65‑1 (MQ=255)
                                                     tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg               >  1:1678013/1‑70 (MQ=255)
                                                       aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                          >  1:1118939/1‑41 (MQ=255)
                                                       aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc            >  1:30463/1‑71 (MQ=255)
                                                         aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                          >  1:3726107/1‑39 (MQ=255)
                                                         aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa                            >  1:336373/1‑53 (MQ=255)
                                                          aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                        >  1:1893518/1‑40 (MQ=255)
                                                            aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg       <  1:393827/71‑1 (MQ=255)
                                                             cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                         >  1:2944199/1‑36 (MQ=255)
                                                             cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt      >  1:1435509/1‑71 (MQ=255)
                                                              gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg     <  1:2385400/71‑3 (MQ=255)
                                                               cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                         >  1:3860512/1‑50 (MQ=255)
                                                               cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg    >  1:2284480/1‑71 (MQ=255)
                                                                 cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc  >  1:61076/1‑71 (MQ=255)
                                                                 |                                                                      
GGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTC  >  minE/833079‑833214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: