Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,150 T→C 7.9% R536R (CGT→CGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1500TC 7.9% 173.4 / 5.6 63R536R (CGT→CGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (39/19);  new base C (3/2);  total (42/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

GTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAT  >  minE/833082‑833218
                                                                    |                                                                    
gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc                                                                    >  1:529407/1‑71 (MQ=255)
gTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTc                                                                    <  1:862890/71‑1 (MQ=255)
   tctcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgat                                                                 >  1:1992396/1‑71 (MQ=255)
            cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                        <  1:3299636/71‑1 (MQ=255)
            cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                        <  1:1469768/71‑1 (MQ=255)
            cAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                        <  1:530504/71‑1 (MQ=255)
             aGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg                                                                   >  1:872914/1‑59 (MQ=255)
              gATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatgat                                                              >  1:3876038/1‑63 (MQ=255)
               attattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCgg                                                     <  1:1306554/71‑1 (MQ=255)
                   ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatga                                                               >  1:875833/1‑57 (MQ=255)
                   ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg                                                 <  1:296428/71‑1 (MQ=255)
                     accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTa                                                            >  1:1762745/3‑58 (MQ=255)
                     accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAt                                                           >  1:3110448/3‑59 (MQ=255)
                     accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCagg                                               <  1:1490025/69‑1 (MQ=255)
                      gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCtgg                                               <  1:1212568/70‑1 (MQ=255)
                      gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCggg                                               <  1:3083936/70‑1 (MQ=255)
                      ccATCGCCCGTGAATTTGCCGCTACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggt                                              >  1:843456/2‑70 (MQ=255)
                        aTCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                            <  1:1204109/71‑1 (MQ=255)
                          cGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc                                                       >  1:557628/1‑58 (MQ=255)
                           gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                         >  1:879762/1‑71 (MQ=255)
                           gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                          <  1:742685/70‑1 (MQ=255)
                           gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                          <  1:3474469/70‑1 (MQ=255)
                           gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                          <  1:3593080/70‑1 (MQ=255)
                               gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc                                                       <  1:1534219/53‑1 (MQ=255)
                               gTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                        >  1:620164/1‑52 (MQ=255)
                                 gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg                                   >  1:718416/1‑71 (MQ=255)
                                 gAATTTGCCGATAACGCTGATAAAACGCACGGGCGTTc                                                                    <  1:1627059/38‑1 (MQ=255)
                                  aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                             >  1:1520512/1‑60 (MQ=255)
                                   aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                  >  1:139358/1‑70 (MQ=255)
                                   aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                  >  1:3614234/1‑70 (MQ=255)
                                   aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                  >  1:1775315/1‑70 (MQ=255)
                                       gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                             >  1:2474027/1‑71 (MQ=255)
                                       gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                             >  1:147388/1‑71 (MQ=255)
                                           aTAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgt                                                        <  1:1002676/40‑1 (MQ=255)
                                           aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                          >  1:1271685/1‑70 (MQ=255)
                                           aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                         <  1:3332809/71‑1 (MQ=255)
                                           aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                         >  1:1047353/1‑71 (MQ=255)
                                           aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                         >  1:1753874/1‑71 (MQ=255)
                                            tAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                         >  1:2822664/1‑70 (MQ=255)
                                             aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                       >  1:3473686/1‑71 (MQ=255)
                                               cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                            >  1:1825987/1‑48 (MQ=255)
                                               cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg                        >  1:2847355/1‑68 (MQ=255)
                                               cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata                     >  1:187174/1‑71 (MQ=255)
                                                gCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                          <  1:1861822/65‑1 (MQ=255)
                                                  tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                   >  1:1678013/1‑70 (MQ=255)
                                                    aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                              >  1:1118939/1‑41 (MQ=255)
                                                    aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                >  1:30463/1‑71 (MQ=255)
                                                      aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                              >  1:3726107/1‑39 (MQ=255)
                                                      aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa                                >  1:336373/1‑53 (MQ=255)
                                                       aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                            >  1:1893518/1‑40 (MQ=255)
                                                         aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg           <  1:393827/71‑1 (MQ=255)
                                                          cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                             >  1:2944199/1‑36 (MQ=255)
                                                          cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt          >  1:1435509/1‑71 (MQ=255)
                                                           gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg         <  1:2385400/71‑3 (MQ=255)
                                                            cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                             >  1:3860512/1‑50 (MQ=255)
                                                            cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg        >  1:2284480/1‑71 (MQ=255)
                                                              cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc      >  1:61076/1‑71 (MQ=255)
                                                                gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg    <  1:3672459/71‑1 (MQ=255)
                                                                 tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                      >  1:2703504/1‑36 (MQ=255)
                                                                 tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                      >  1:3008592/1‑36 (MQ=255)
                                                                  cGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                             >  1:455290/1‑44 (MQ=255)
                                                                   gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt  >  1:281384/1‑70 (MQ=255)
                                                                   gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt  >  1:3471688/1‑70 (MQ=255)
                                                                    ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt                               <  1:1330982/40‑1 (MQ=255)
                                                                    ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt  <  1:296129/69‑1 (MQ=255)
                                                                    |                                                                    
GTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAT  >  minE/833082‑833218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: