Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 189,622 G→T 9.5% S373S (TCG→TCT ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE189,6220GT 9.5% 200.0 / 5.5 63S373S (TCG→TCTldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (24/33);  new base T (4/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGC  >  minE/189552‑189689
                                                                      |                                                                    
gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg                                                                      <  1:1353903/71‑1 (MQ=255)
  ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa                                                                    <  1:3518502/71‑1 (MQ=255)
  ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa                                                                    <  1:3767439/71‑1 (MQ=255)
  ggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa                                                                    <  1:168812/71‑1 (MQ=255)
   gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGCCGGCGTTATCGCa                                                                    <  1:2304605/70‑1 (MQ=255)
        cGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc                                                              >  1:602715/1‑71 (MQ=255)
           gtTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCtt                                                               <  1:3125983/67‑1 (MQ=255)
            ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCTACAAAATGCTGGCGGCGTTATCGCAGGCt                                                                <  1:598359/65‑1 (MQ=255)
            ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt                                                           >  1:2457588/1‑70 (MQ=255)
             tGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg                                                          <  1:555725/70‑1 (MQ=255)
                gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATc                                                       >  1:875236/1‑70 (MQ=255)
                 gggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca                                                     >  1:1732390/4‑71 (MQ=255)
                 gggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca                                                     >  1:3431554/4‑71 (MQ=255)
                  gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcac                                                    <  1:1232237/71‑1 (MQ=255)
                   aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcaca                                                   <  1:2900179/71‑1 (MQ=255)
                    aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg                                                                      <  1:2430194/51‑1 (MQ=255)
                     aaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt                                                  >  1:3542458/1‑70 (MQ=255)
                      aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa                                                <  1:1927645/71‑1 (MQ=255)
                       gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa                                                >  1:2282388/1‑70 (MQ=255)
                          aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa                                              <  1:3768447/69‑1 (MQ=255)
                          aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa                                                <  1:238895/67‑1 (MQ=255)
                             ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg                                                          <  1:2168222/54‑1 (MQ=255)
                             ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg                                            >  1:2955028/1‑68 (MQ=255)
                             ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc                                           >  1:754662/1‑69 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTTATCCACATTAAAGGc                                           <  1:3926652/67‑1 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc                                           <  1:375758/67‑1 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc                                           <  1:1068284/67‑1 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg                                          <  1:1811161/68‑1 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                       <  1:2076571/70‑1 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                       >  1:3454258/1‑70 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                       >  1:1049595/1‑70 (MQ=255)
                               cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTAGCTGATCCACATTAAAGGCg                                          <  1:2447914/68‑1 (MQ=255)
                                  aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATg                                   <  1:2130320/71‑1 (MQ=255)
                                   aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa                                  >  1:1912756/1‑71 (MQ=255)
                                   aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa                                  >  1:2772430/1‑71 (MQ=255)
                                    cGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg                                          <  1:3825564/63‑1 (MQ=255)
                                        aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                >  1:3144947/1‑68 (MQ=255)
                                         tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                              >  1:1795071/1‑69 (MQ=255)
                                         tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                              >  1:30969/1‑69 (MQ=255)
                                              ccACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGc                         >  1:577684/1‑69 (MQ=255)
                                               cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATCACGAAGAGGCCtt                      >  1:2614385/1‑71 (MQ=255)
                                                acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                     <  1:3878029/71‑1 (MQ=255)
                                                 caAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTa                    <  1:106517/71‑1 (MQ=255)
                                                 caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                     >  1:342552/1‑70 (MQ=255)
                                                 caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                     >  1:1440890/1‑70 (MQ=255)
                                                 caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                     >  1:109014/1‑70 (MQ=255)
                                                  aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTaa                   >  1:3327390/1‑71 (MQ=255)
                                                  aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTaa                   >  1:3151622/1‑71 (MQ=255)
                                                  aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGa                               >  1:3602599/1‑59 (MQ=255)
                                                   aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAAc                  <  1:468205/71‑1 (MQ=255)
                                                    aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACg                 <  1:2524304/71‑1 (MQ=255)
                                                    aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACg                 <  1:1989331/71‑1 (MQ=255)
                                                     aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTat                                               <  1:2838230/41‑2 (MQ=255)
                                                      tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg                                          >  1:489712/1‑45 (MQ=255)
                                                      tGCTGGCGGCGTTATCGCAGGCTTCGCTGATACACATTAAAGGCGAGTATGACGAag                             >  1:874904/1‑57 (MQ=255)
                                                        cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAg              <  1:949683/70‑1 (MQ=255)
                                                        cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAg              <  1:1421299/70‑1 (MQ=255)
                                                        cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAg              <  1:1100066/70‑1 (MQ=255)
                                                           gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCt           <  1:1810438/70‑1 (MQ=9)
                                                              gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTat       >  1:1164167/1‑71 (MQ=255)
                                                                 ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                              <  1:2776494/45‑1 (MQ=255)
                                                                 ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                              <  1:1195601/45‑1 (MQ=255)
                                                                 ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat    <  1:3050058/71‑1 (MQ=255)
                                                                   aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg   >  1:541136/1‑70 (MQ=255)
                                                                    tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGc  <  1:1846554/70‑1 (MQ=255)
                                                                      |                                                                    
GCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGC  >  minE/189552‑189689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: