Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,168 T→C 13.3% G542G (GGT→GGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1680TC13.3% 149.3 / 13.3 60G542G (GGT→GGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (37/15);  new base C (4/4);  total (41/19)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGC  >  minE/833101‑833237
                                                                   |                                                                     
ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg                                                                    <  1:296428/71‑1 (MQ=255)
  accATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCagg                                                                  <  1:1490025/69‑1 (MQ=255)
   gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCtgg                                                                  <  1:1212568/70‑1 (MQ=255)
   gCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCggg                                                                  <  1:3083936/70‑1 (MQ=255)
   ccATCGCCCGTGAATTTGCCGCTACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggt                                                                 >  1:843456/2‑70 (MQ=255)
     aTCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                                               <  1:1204109/71‑1 (MQ=255)
        gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                                            >  1:879762/1‑71 (MQ=255)
        gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                             <  1:742685/70‑1 (MQ=255)
        gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                             <  1:3593080/70‑1 (MQ=255)
        gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                             <  1:3474469/70‑1 (MQ=255)
              gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg                                                      >  1:718416/1‑71 (MQ=255)
               aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                                                >  1:1520512/1‑60 (MQ=255)
                aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                     >  1:139358/1‑70 (MQ=255)
                aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                     >  1:3614234/1‑70 (MQ=255)
                aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                     >  1:1775315/1‑70 (MQ=255)
                    gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                >  1:147388/1‑71 (MQ=255)
                    gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                >  1:2474027/1‑71 (MQ=255)
                        aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                                             >  1:1271685/1‑70 (MQ=255)
                        aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                            >  1:1753874/1‑71 (MQ=255)
                        aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                            <  1:3332809/71‑1 (MQ=255)
                        aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                            >  1:1047353/1‑71 (MQ=255)
                         tAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                            >  1:2822664/1‑70 (MQ=255)
                          aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                          >  1:3473686/1‑71 (MQ=255)
                            cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                                               >  1:1825987/1‑48 (MQ=255)
                            cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg                                           >  1:2847355/1‑68 (MQ=255)
                            cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata                                        >  1:187174/1‑71 (MQ=255)
                             gCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                                             <  1:1861822/65‑1 (MQ=255)
                               tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                                      >  1:1678013/1‑70 (MQ=255)
                                 aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                                                 >  1:1118939/1‑41 (MQ=255)
                                 aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                   >  1:30463/1‑71 (MQ=255)
                                   aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCgggg                                                                 >  1:3726107/1‑39 (MQ=255)
                                   aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa                                                   >  1:336373/1‑53 (MQ=255)
                                    aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                                               >  1:1893518/1‑40 (MQ=255)
                                      aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                              <  1:393827/71‑1 (MQ=255)
                                       cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGc                                                                >  1:2944199/1‑36 (MQ=255)
                                       cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                             >  1:1435509/1‑71 (MQ=255)
                                        gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg                            <  1:2385400/71‑3 (MQ=255)
                                         cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                >  1:3860512/1‑50 (MQ=255)
                                         cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                           >  1:2284480/1‑71 (MQ=255)
                                           cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                         >  1:61076/1‑71 (MQ=255)
                                             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                       <  1:3672459/71‑1 (MQ=255)
                                              tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                                         >  1:2703504/1‑36 (MQ=255)
                                              tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                                         >  1:3008592/1‑36 (MQ=255)
                                               cGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                >  1:455290/1‑44 (MQ=255)
                                                gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGgatgat                     >  1:3920848/3‑70 (MQ=255)
                                                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                     >  1:3471688/1‑70 (MQ=255)
                                                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                     >  1:281384/1‑70 (MQ=255)
                                                 ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt                                                  <  1:1330982/40‑1 (MQ=255)
                                                 ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                     <  1:296129/69‑1 (MQ=255)
                                                  tCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATgta                                    <  1:1583876/53‑3 (MQ=255)
                                                  tCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCa                                                <  1:3749859/41‑1 (MQ=12)
                                                   cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                   >  1:241407/1‑69 (MQ=255)
                                                    gatgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACa                >  1:3254207/1‑71 (MQ=255)
                                                    gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                >  1:349241/1‑69 (MQ=255)
                                                    gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                >  1:1961334/1‑69 (MQ=255)
                                                     atgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt               >  1:1304343/1‑71 (MQ=255)
                                                       gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGat                                         >  1:2491208/1‑43 (MQ=255)
                                                       gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc             >  1:1351504/1‑71 (MQ=255)
                                                        atTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGAGCAATATGc             <  1:1822440/70‑1 (MQ=255)
                                                                  gTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc  <  1:3068470/71‑1 (MQ=255)
                                                                   |                                                                     
TTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGC  >  minE/833101‑833237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: