Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,177 G→T 10.7% L545L (CTG→CTT) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1770GT10.7% 173.3 / 5.6 56L545L (CTG→CTT) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (32/18);  new base T (3/3);  total (35/21)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGT  >  minE/833109‑833245
                                                                    |                                                                    
gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                                                    >  1:879762/1‑71 (MQ=255)
gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                                     <  1:3593080/70‑1 (MQ=255)
gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                                     <  1:3474469/70‑1 (MQ=255)
gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                                     <  1:742685/70‑1 (MQ=255)
      gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg                                                              >  1:718416/1‑71 (MQ=255)
        aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                             >  1:3614234/1‑70 (MQ=255)
        aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                             >  1:139358/1‑70 (MQ=255)
        aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                             >  1:1775315/1‑70 (MQ=255)
            gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                        >  1:147388/1‑71 (MQ=255)
            gCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                        >  1:2474027/1‑71 (MQ=255)
                aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                                                     >  1:1271685/1‑70 (MQ=255)
                aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                    >  1:1047353/1‑71 (MQ=255)
                aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                    <  1:3332809/71‑1 (MQ=255)
                aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                    >  1:1753874/1‑71 (MQ=255)
                 tAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                    >  1:2822664/1‑70 (MQ=255)
                  aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                                  >  1:3473686/1‑71 (MQ=255)
                    cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg                                                   >  1:2847355/1‑68 (MQ=255)
                    cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata                                                >  1:187174/1‑71 (MQ=255)
                     gCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCt                                                     <  1:1861822/65‑1 (MQ=255)
                       tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                                              >  1:1678013/1‑70 (MQ=255)
                         aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                           >  1:30463/1‑71 (MQ=255)
                           aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa                                                           >  1:336373/1‑53 (MQ=255)
                              aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                      <  1:393827/71‑1 (MQ=255)
                               cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                     >  1:1435509/1‑71 (MQ=255)
                                gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg                                    <  1:2385400/71‑3 (MQ=255)
                                 cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                        >  1:3860512/1‑50 (MQ=255)
                                 cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                   >  1:2284480/1‑71 (MQ=255)
                                   cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                 >  1:61076/1‑71 (MQ=255)
                                     gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                               <  1:3672459/71‑1 (MQ=255)
                                      tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                                                 >  1:3008592/1‑36 (MQ=255)
                                      tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                                                 >  1:2703504/1‑36 (MQ=255)
                                       cGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCa                                                        >  1:455290/1‑44 (MQ=255)
                                        gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGgatgat                             >  1:3920848/3‑70 (MQ=255)
                                        gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                             >  1:3471688/1‑70 (MQ=255)
                                        gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                             >  1:281384/1‑70 (MQ=255)
                                         ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt                                                          <  1:1330982/40‑1 (MQ=255)
                                         ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                             <  1:296129/69‑1 (MQ=255)
                                          tCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATgta                                            <  1:1583876/53‑3 (MQ=255)
                                          tCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCa                                                        <  1:3749859/41‑1 (MQ=12)
                                           cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                           >  1:241407/1‑69 (MQ=255)
                                            gatgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACa                        >  1:3254207/1‑71 (MQ=255)
                                            gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                        >  1:1961334/1‑69 (MQ=255)
                                            gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                        >  1:349241/1‑69 (MQ=255)
                                             atgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                       >  1:1304343/1‑71 (MQ=255)
                                               gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGat                                                 >  1:2491208/1‑43 (MQ=255)
                                               gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                     >  1:1351504/1‑71 (MQ=255)
                                                atTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGAGCAATATGc                     <  1:1822440/70‑1 (MQ=255)
                                                          gTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc          <  1:3068470/71‑1 (MQ=255)
                                                             cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTTGTCTGATCAACATGCTGATTTTCTGAGGc       <  1:3184480/71‑1 (MQ=255)
                                                             cGGGGCTGAACCACTGGTAT‑‑CCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg     <  1:3312301/71‑1 (MQ=255)
                                                              ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                           <  1:115143/50‑1 (MQ=255)
                                                              ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc          <  1:2087285/67‑1 (MQ=255)
                                                               gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg     >  1:327411/1‑71 (MQ=255)
                                                                 gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                <  1:2900878/42‑1 (MQ=255)
                                                                  cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt  <  1:3901796/71‑1 (MQ=255)
                                                                  cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt  <  1:87909/71‑1 (MQ=255)
                                                                    gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATATTCTGCGGCtg     >  1:2957393/1‑66 (MQ=255)
                                                                    |                                                                    
GCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGT  >  minE/833109‑833245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: