Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,198 T→C 10.8% D552D (GAT→GAC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1980TC10.8% 121.3 / 6.0 46D552D (GAT→GACnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base C (3/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTG  >  minE/833129‑833266
                                                                     |                                                                    
cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGata                                                                     >  1:187174/1‑71 (MQ=255)
   tGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                                                                   >  1:1678013/1‑70 (MQ=255)
     aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                                                >  1:30463/1‑71 (MQ=255)
          aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                           <  1:393827/71‑1 (MQ=255)
           cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                                          >  1:1435509/1‑71 (MQ=255)
            gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg                                                         <  1:2385400/71‑3 (MQ=255)
             cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                        >  1:2284480/1‑71 (MQ=255)
               cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                      >  1:61076/1‑71 (MQ=255)
                 gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                    <  1:3672459/71‑1 (MQ=255)
                    gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGgatgat                                                  >  1:3920848/3‑70 (MQ=255)
                    gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                  >  1:3471688/1‑70 (MQ=255)
                    gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                  >  1:281384/1‑70 (MQ=255)
                     ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                  <  1:296129/69‑1 (MQ=255)
                      tCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATgta                                                                 <  1:1583876/53‑3 (MQ=255)
                       cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                >  1:241407/1‑69 (MQ=255)
                        gatgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACa                                             >  1:3254207/1‑71 (MQ=255)
                        gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                                             >  1:1961334/1‑69 (MQ=255)
                        gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                                             >  1:349241/1‑69 (MQ=255)
                         atgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                            >  1:1304343/1‑71 (MQ=255)
                           gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGat                                                                      >  1:2491208/1‑43 (MQ=255)
                           gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                          >  1:1351504/1‑71 (MQ=255)
                            atTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGAGCAATATGc                                          <  1:1822440/70‑1 (MQ=255)
                                      gTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                               <  1:3068470/71‑1 (MQ=255)
                                         cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTTGTCTGATCAACATGCTGATTTTCTGAGGc                            <  1:3184480/71‑1 (MQ=255)
                                         cGGGGCTGAACCACTGGTAT‑‑CCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                          <  1:3312301/71‑1 (MQ=255)
                                          ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                <  1:115143/50‑1 (MQ=255)
                                          ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                               <  1:2087285/67‑1 (MQ=255)
                                           gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                          >  1:327411/1‑71 (MQ=255)
                                             gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                     <  1:2900878/42‑1 (MQ=255)
                                              cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                       <  1:3901796/71‑1 (MQ=255)
                                              cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                       <  1:87909/71‑1 (MQ=255)
                                                gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATATTCTGCGGCtg                          >  1:2957393/1‑66 (MQ=255)
                                                 aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                    >  1:3377018/1‑71 (MQ=255)
                                                 aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                    >  1:2823613/1‑71 (MQ=255)
                                                  aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                               <  1:2092011/59‑1 (MQ=255)
                                                  aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                   <  1:2039939/71‑1 (MQ=255)
                                                  aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                   <  1:532157/71‑1 (MQ=255)
                                                    cACTGGTATCACCTCGATATGATCTATCGTGGTCTGATCAACATGCTGAttt                                    <  1:2263478/52‑1 (MQ=255)
                                                    cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAttt                                    <  1:2944246/52‑1 (MQ=255)
                                                    cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                 <  1:2452171/71‑1 (MQ=255)
                                                     aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                <  1:229776/71‑1 (MQ=255)
                                                      cTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg                        <  1:3141028/62‑1 (MQ=255)
                                                           aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg                             <  1:2167149/52‑1 (MQ=255)
                                                           aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg          >  1:3192615/1‑71 (MQ=255)
                                                            tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg         >  1:3019126/1‑71 (MQ=255)
                                                                  cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt   >  1:707151/1‑71 (MQ=255)
                                                                   gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt   <  1:593856/70‑1 (MQ=255)
                                                                   gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg  <  1:2358624/71‑1 (MQ=255)
                                                                    atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt                         <  1:455751/47‑1 (MQ=255)
                                                                     |                                                                    
CGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTG  >  minE/833129‑833266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: