Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,207 T→C 10.4% Y555Y (TAT→TAC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,2070TC10.4% 126.5 / 5.4 48Y555Y (TAT→TACnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (17/26);  new base C (3/2);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATG  >  minE/833139‑833277
                                                                    |                                                                      
aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                                      <  1:393827/71‑1 (MQ=255)
 cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                                                     >  1:1435509/1‑71 (MQ=255)
  gCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCgcg                                                                    <  1:2385400/71‑3 (MQ=255)
   cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                                   >  1:2284480/1‑71 (MQ=255)
     cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 >  1:61076/1‑71 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                               <  1:3672459/71‑1 (MQ=255)
          gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGgatgat                                                             >  1:3920848/3‑70 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                             >  1:281384/1‑70 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                             >  1:3471688/1‑70 (MQ=255)
           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                             <  1:296129/69‑1 (MQ=255)
             cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                           >  1:241407/1‑69 (MQ=255)
              gatgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACa                                                        >  1:3254207/1‑71 (MQ=255)
              gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                                                        >  1:1961334/1‑69 (MQ=255)
              gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                                                        >  1:349241/1‑69 (MQ=255)
               atgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                                       >  1:1304343/1‑71 (MQ=255)
                 gatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                                     >  1:1351504/1‑71 (MQ=255)
                  atTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGAGCAATATGc                                                     <  1:1822440/70‑1 (MQ=255)
                            gTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                          <  1:3068470/71‑1 (MQ=255)
                               cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTTGTCTGATCAACATGCTGATTTTCTGAGGc                                       <  1:3184480/71‑1 (MQ=255)
                               cGGGGCTGAACCACTGGTAT‑‑CCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                                     <  1:3312301/71‑1 (MQ=255)
                                ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                           <  1:115143/50‑1 (MQ=255)
                                ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                          <  1:2087285/67‑1 (MQ=255)
                                 gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                                     >  1:327411/1‑71 (MQ=255)
                                   gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                                <  1:2900878/42‑1 (MQ=255)
                                    cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                                  <  1:3901796/71‑1 (MQ=255)
                                    cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                                  <  1:87909/71‑1 (MQ=255)
                                      gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATATTCTGCGGCtg                                     >  1:2957393/1‑66 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                               >  1:3377018/1‑71 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                               >  1:2823613/1‑71 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                          <  1:2092011/59‑1 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                              <  1:2039939/71‑1 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                              <  1:532157/71‑1 (MQ=255)
                                          cACTGGTATCACCTCGATATGATCTATCGTGGTCTGATCAACATGCTGAttt                                               <  1:2263478/52‑1 (MQ=255)
                                          cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAttt                                               <  1:2944246/52‑1 (MQ=255)
                                          cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                            <  1:2452171/71‑1 (MQ=255)
                                           aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:229776/71‑1 (MQ=255)
                                            cTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg                                   <  1:3141028/62‑1 (MQ=255)
                                                 aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg                                        <  1:2167149/52‑1 (MQ=255)
                                                 aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg                     >  1:3192615/1‑71 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg                    >  1:3019126/1‑71 (MQ=255)
                                                        cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt              >  1:707151/1‑71 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt              <  1:593856/70‑1 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg             <  1:2358624/71‑1 (MQ=255)
                                                          atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt                                    <  1:455751/47‑1 (MQ=255)
                                                             tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                            >  1:2618477/1‑52 (MQ=255)
                                                             tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGgc          <  1:1425439/70‑1 (MQ=255)
                                                              gAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggggg                   <  1:2117250/60‑1 (MQ=255)
                                                               aaCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc                        <  1:3379657/54‑1 (MQ=255)
                                                                    tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg  >  1:3229928/1‑71 (MQ=255)
                                                                    |                                                                      
ACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATG  >  minE/833139‑833277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: