Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,231 T→C 7.4% I563I (ATT→ATC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,2310TC 7.4% 150.1 / 3.4 55I563I (ATT→ATCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base C (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGT  >  minE/833167‑833297
                                                                |                                                                  
gTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                                              <  1:3068470/71‑1 (MQ=255)
   cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTTGTCTGATCAACATGCTGATTTTCTGAGGc                                                           <  1:3184480/71‑1 (MQ=255)
   cGGGGCTGAACCACTGGTAT‑‑CCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                                                         <  1:3312301/71‑1 (MQ=255)
    ggggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                                              <  1:2087285/67‑1 (MQ=255)
     gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg                                                         >  1:327411/1‑71 (MQ=255)
        cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                                                      <  1:3901796/71‑1 (MQ=255)
        cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                                                      <  1:87909/71‑1 (MQ=255)
          gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATATTCTGCGGCtg                                                         >  1:2957393/1‑66 (MQ=255)
           aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                                                   >  1:2823613/1‑71 (MQ=255)
           aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                                                   >  1:3377018/1‑71 (MQ=255)
            aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGc                                                              <  1:2092011/59‑1 (MQ=255)
            aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                                                  <  1:532157/71‑1 (MQ=255)
            aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                                                  <  1:2039939/71‑1 (MQ=255)
              cACTGGTATCACCTCGATATGATCTATCGTGGTCTGATCAACATGCTGAttt                                                                   <  1:2263478/52‑1 (MQ=255)
              cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAttt                                                                   <  1:2944246/52‑1 (MQ=255)
              cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                                                <  1:2452171/71‑1 (MQ=255)
               aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                                               <  1:229776/71‑1 (MQ=255)
                cTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg                                                       <  1:3141028/62‑1 (MQ=255)
                     aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg                                                            <  1:2167149/52‑1 (MQ=255)
                     aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg                                         >  1:3192615/1‑71 (MQ=255)
                      tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg                                        >  1:3019126/1‑71 (MQ=255)
                            cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt                                  >  1:707151/1‑71 (MQ=255)
                             gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt                                  <  1:593856/70‑1 (MQ=255)
                             gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg                                 <  1:2358624/71‑1 (MQ=255)
                              atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt                                                        <  1:455751/47‑1 (MQ=255)
                                 tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                                                >  1:2618477/1‑52 (MQ=255)
                                 tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGgc                              <  1:1425439/70‑1 (MQ=255)
                                  gAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggggg                                       <  1:2117250/60‑1 (MQ=255)
                                   aaCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc                                            <  1:3379657/54‑1 (MQ=255)
                                        tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg                      >  1:3229928/1‑71 (MQ=255)
                                         cGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg                      >  1:937595/1‑70 (MQ=255)
                                          gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg                                                   >  1:1841227/1‑40 (MQ=255)
                                          gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                                                  >  1:2489336/1‑41 (MQ=255)
                                          gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGt                     <  1:3169175/70‑1 (MQ=255)
                                           tGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg                                                    <  1:1027163/38‑1 (MQ=255)
                                           tGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg                                                    <  1:3307607/38‑1 (MQ=255)
                                             gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg                                        >  1:2546758/1‑48 (MQ=255)
                                                  aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAg              >  1:1017707/1‑69 (MQ=255)
                                                  aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCg                                           <  1:235335/40‑1 (MQ=255)
                                                  aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg                                    >  1:2649595/1‑47 (MQ=255)
                                                  aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg                                    >  1:2610281/1‑47 (MQ=255)
                                                  aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg                                    >  1:514187/1‑47 (MQ=255)
                                                  aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg                                    >  1:2196467/1‑47 (MQ=255)
                                                    cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa           >  1:101332/1‑70 (MQ=255)
                                                    cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa           >  1:2285684/1‑70 (MQ=255)
                                                    cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa           >  1:1672617/1‑70 (MQ=255)
                                                     aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaa         <  1:2775460/71‑1 (MQ=255)
                                                      aCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaaa        <  1:2876246/71‑1 (MQ=255)
                                                      aCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaaa        <  1:3908201/71‑1 (MQ=255)
                                                       cATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGt                     >  1:14721/1‑57 (MQ=255)
                                                       cATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc       >  1:2136151/1‑71 (MQ=255)
                                                        aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACt      >  1:1388559/1‑71 (MQ=255)
                                                         tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg     >  1:2992050/1‑71 (MQ=255)
                                                         tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg     <  1:2421184/71‑1 (MQ=255)
                                                           cTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCg   <  1:1008393/71‑1 (MQ=255)
                                                            tGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGgc                              <  1:320456/43‑1 (MQ=255)
                                                             gATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGt  >  1:3474505/1‑70 (MQ=255)
                                                                |                                                                  
GTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGT  >  minE/833167‑833297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: