| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,240 | C→T | 9.1% | G566G (GGC→GGT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,240 | 0 | C | T | 9.1% | 157.5 / 5.7 | 55 | G566G (GGC→GGT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (25/25); new base T (2/3); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACC > minE/833170‑833306 | cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTTGTCTGATCAACATGCTGATTTTCTGAGGc < 1:3184480/71‑1 (MQ=255)cGGGGCTGAACCACTGGTAT‑‑CCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg < 1:3312301/71‑1 (MQ=255) gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtg > 1:327411/1‑71 (MQ=255) cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt < 1:87909/71‑1 (MQ=255) cTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt < 1:3901796/71‑1 (MQ=255) gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATATTCTGCGGCtg > 1:2957393/1‑66 (MQ=255) aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg > 1:2823613/1‑71 (MQ=255) aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg > 1:3377018/1‑71 (MQ=255) aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt < 1:532157/71‑1 (MQ=255) aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt < 1:2039939/71‑1 (MQ=255) cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa < 1:2452171/71‑1 (MQ=255) aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:229776/71‑1 (MQ=255) cTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg < 1:3141028/62‑1 (MQ=255) aTCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg > 1:3192615/1‑71 (MQ=255) tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg > 1:3019126/1‑71 (MQ=255) cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt > 1:707151/1‑71 (MQ=255) gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt < 1:593856/70‑1 (MQ=255) gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg < 1:2358624/71‑1 (MQ=255) atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt < 1:455751/47‑1 (MQ=255) tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa > 1:2618477/1‑52 (MQ=255) tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGgc < 1:1425439/70‑1 (MQ=255) gAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggggg < 1:2117250/60‑1 (MQ=255) aaCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc < 1:3379657/54‑1 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:3229928/1‑71 (MQ=255) cGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:937595/1‑70 (MQ=255) gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCgg > 1:1841227/1‑40 (MQ=255) gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt > 1:2489336/1‑41 (MQ=255) gTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGt < 1:3169175/70‑1 (MQ=255) tGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg < 1:1027163/38‑1 (MQ=255) tGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg < 1:3307607/38‑1 (MQ=255) gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgggg > 1:2546758/1‑48 (MQ=255) aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAg > 1:1017707/1‑69 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCg < 1:235335/40‑1 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg > 1:2649595/1‑47 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg > 1:514187/1‑47 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg > 1:2196467/1‑47 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg > 1:2610281/1‑47 (MQ=255) cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa > 1:2285684/1‑70 (MQ=255) cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa > 1:101332/1‑70 (MQ=255) cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaa > 1:1672617/1‑70 (MQ=255) aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaa < 1:2775460/71‑1 (MQ=255) aCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaaa < 1:2876246/71‑1 (MQ=255) aCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaaaa < 1:3908201/71‑1 (MQ=255) cATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGt > 1:14721/1‑57 (MQ=255) cATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:2136151/1‑71 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACt > 1:1388559/1‑71 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg < 1:2421184/71‑1 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg > 1:2992050/1‑71 (MQ=255) cTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCg < 1:1008393/71‑1 (MQ=255) tGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGgc < 1:320456/43‑1 (MQ=255) gATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGt > 1:3474505/1‑70 (MQ=255) tCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:3760383/57‑1 (MQ=255) cTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCcaca < 1:780955/69‑1 (MQ=255) cTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCaa < 1:2464834/70‑1 (MQ=255) gCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAAcc > 1:2004451/1‑71 (MQ=255) gCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:1182187/51‑1 (MQ=255) | CGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACC > minE/833170‑833306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |