| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,847 | G→C | 8.9% | V769L (GTG→CTG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,847 | 0 | G | C | 8.9% | 156.6 / 5.7 | 56 | V769L (GTG→CTG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (30/21); new base C (2/3); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTG > minE/833777‑833917 | gCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCtgg < 1:1579500/71‑1 (MQ=255) aCAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCtggtg < 1:3848923/70‑1 (MQ=255) ggcggcGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTAc > 1:1042999/1‑71 (MQ=255) ggcggcGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTAc > 1:353916/1‑71 (MQ=255) ggcggcGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTAc > 1:93836/1‑71 (MQ=255) gTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGa > 1:3922503/1‑71 (MQ=255) gTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGa > 1:2099251/1‑71 (MQ=255) tGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCtgg < 1:218400/57‑1 (MQ=255) tGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGc > 1:1420810/1‑66 (MQ=255) aaGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACtt > 1:2965366/1‑71 (MQ=255) aGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCtggtg > 1:1121658/1‑56 (MQ=255) aGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCtggtg > 1:1397650/1‑56 (MQ=255) aGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACtt < 1:662166/70‑1 (MQ=255) ggAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTc < 1:1820759/71‑1 (MQ=255) aagTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTc < 1:3367957/70‑1 (MQ=255) gTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAg > 1:170765/1‑71 (MQ=255) gTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAg < 1:1867046/71‑1 (MQ=255) gACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCAc < 1:353209/71‑1 (MQ=255) ggCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGt > 1:2150872/1‑71 (MQ=255) gCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTg < 1:3879027/69‑1 (MQ=255) gCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGtc < 1:2423677/71‑1 (MQ=255) cAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGtct < 1:841087/71‑1 (MQ=255) ggACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTg < 1:3636206/43‑1 (MQ=255) ggACAATGGTCTGGAAGGCAAGCTGGATCTGCTGGTTACGCTg < 1:1559453/43‑1 (MQ=255) gACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTg < 1:2608539/54‑1 (MQ=255) gACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTa > 1:1941801/1‑71 (MQ=255) aCAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACtt < 1:920669/46‑1 (MQ=255) cAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTa > 1:230671/1‑35 (MQ=255) cAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTa > 1:1091855/1‑35 (MQ=255) cAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGtctc > 1:3766115/1‑67 (MQ=255) aaTGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTcc > 1:2844672/1‑46 (MQ=255) aaTGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTcc > 1:3195794/1‑46 (MQ=255) aaTGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTcc > 1:2038522/1‑46 (MQ=255) gTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGa > 1:517880/1‑70 (MQ=255) gTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGa > 1:628468/1‑70 (MQ=255) tCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCg < 1:1354309/49‑1 (MQ=255) tttgAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGa < 1:1693588/65‑1 (MQ=255) attgAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAGTACATGCCTGTTCTCCGatat < 1:78509/68‑1 (MQ=255) tGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcat > 1:3906359/1‑70 (MQ=255) tGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcat > 1:1794463/1‑70 (MQ=255) gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCAc > 1:562351/1‑50 (MQ=255) gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcatca < 1:2543467/70‑1 (MQ=255) gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcataat > 1:1805447/1‑71 (MQ=255) gAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAg > 1:1254039/1‑47 (MQ=255) gAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAg > 1:2319323/1‑47 (MQ=255) aaGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCa < 1:843662/48‑1 (MQ=255) aaGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTc > 1:3540995/1‑61 (MQ=255) aGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAttt < 1:2103152/71‑1 (MQ=255) aGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAttt < 1:2738845/71‑1 (MQ=255) aGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAtt > 1:2705971/1‑70 (MQ=255) ggCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAtttt < 1:3019309/71‑1 (MQ=255) gCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGtctc < 1:142174/53‑1 (MQ=255) gCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCg < 1:2905222/60‑1 (MQ=255) gCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTg > 1:465022/1‑71 (MQ=255) cAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTa > 1:337403/1‑54 (MQ=255) aGCTGGATCTGGTGGTTACCCTGGACTTCCGTCTGTCGAGCACCTGTCTCTa > 1:2885293/1‑52 (MQ=255) gCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGa > 1:996399/1‑71 (MQ=255) cTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAc < 1:2875369/71‑1 (MQ=255) tCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTAt < 1:476899/46‑1 (MQ=255) tCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGa > 1:1051907/1‑71 (MQ=255) cTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGAc > 1:1257131/1‑71 (MQ=255) tggtggTTACGCTGGACTTCCGTCTGTCGAGCACCTGtctc < 1:2172931/41‑1 (MQ=255) tggtggTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAc > 1:2466527/1‑64 (MQ=255) gtggtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCt < 1:3694872/70‑1 (MQ=255) gtggtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTg > 1:1699652/1‑71 (MQ=255) | GCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTG > minE/833777‑833917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |