| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,641 | A→G | 9.2% | I380V (ATT→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,641 | 0 | A | G | 9.2% | 146.5 / 5.2 | 54 | I380V (ATT→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (26/23); new base G (3/2); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTT > minE/189571‑189710 | aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcaca < 1:2900179/71‑1 (MQ=255) aaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:3542458/1‑70 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1927645/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa > 1:2282388/1‑70 (MQ=255) aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa < 1:3768447/69‑1 (MQ=255) aTCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:238895/67‑1 (MQ=255) ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:2955028/1‑68 (MQ=255) ttCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc > 1:754662/1‑69 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTTATCCACATTAAAGGc < 1:3926652/67‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:1068284/67‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:375758/67‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:1811161/68‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:1049595/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:2076571/70‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:3454258/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTAGCTGATCCACATTAAAGGCg < 1:2447914/68‑1 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATg < 1:2130320/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:1912756/1‑71 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:2772430/1‑71 (MQ=255) cGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:3825564/63‑1 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg > 1:3144947/1‑68 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:30969/1‑69 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:1795071/1‑69 (MQ=255) ccACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGc > 1:577684/1‑69 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATCACGAAGAGGCCtt > 1:2614385/1‑71 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:3878029/71‑1 (MQ=255) caAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTa < 1:106517/71‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:109014/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:1440890/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:342552/1‑70 (MQ=255) aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTaa > 1:3151622/1‑71 (MQ=255) aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTaa > 1:3327390/1‑71 (MQ=255) aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGa > 1:3602599/1‑59 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAAc < 1:468205/71‑1 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg < 1:2524304/71‑1 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg < 1:1989331/71‑1 (MQ=255) aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTat < 1:2838230/41‑2 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg > 1:489712/1‑45 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATACACATTAAAGGCGA‑GTATGACGAag > 1:874904/1‑57 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:949683/70‑1 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:1100066/70‑1 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:1421299/70‑1 (MQ=255) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCt < 1:1810438/70‑1 (MQ=9) gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat > 1:1164167/1‑71 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2776494/45‑1 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:1195601/45‑1 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat < 1:3050058/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:541136/1‑70 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGc < 1:1846554/70‑1 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGc > 1:1171744/1‑37 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc > 1:2429243/1‑71 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc > 1:1936249/1‑71 (MQ=255) gCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCt > 1:1620737/1‑71 (MQ=255) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc > 1:2163227/2‑71 (MQ=9) aTCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCg > 1:608139/1‑69 (MQ=255) cacaTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAg < 1:3307876/71‑1 (MQ=255) acaTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCa > 1:3013327/1‑69 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGtt < 1:723364/71‑1 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGtt < 1:2203421/71‑1 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGt > 1:14578/1‑70 (MQ=255) | AAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTT > minE/189571‑189710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |