| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,933 | C→T | 36.2% | D797D (GAC→GAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,933 | 0 | C | T | 36.2% | 57.3 / 53.7 | 58 | D797D (GAC→GAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/19); new base T (10/11); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG > minE/833871‑834000 | tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa < 1:3617365/68‑1 (MQ=255)tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATAc > 1:1785461/1‑71 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACtt > 1:1507433/1‑71 (MQ=255) tGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATg < 1:2630184/57‑1 (MQ=255) ctctATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCa > 1:1652325/1‑71 (MQ=255) ttctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCAt < 1:2153056/66‑1 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa < 1:601654/51‑1 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg < 1:2145552/70‑1 (MQ=255) tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt > 1:729285/1‑71 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATc > 1:406587/1‑66 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGa > 1:2581712/1‑57 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa < 1:1145252/71‑1 (MQ=255) cGATATCGTTCTGCCCACCGCCACCT‑GTACGAAAAAGACGATATGAACACCTCGGATATGc > 1:684424/1‑61 (MQ=255) gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt < 1:3459817/67‑1 (MQ=255) gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt < 1:2051805/67‑1 (MQ=255) atatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCAt > 1:3230386/3‑62 (MQ=255) tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc < 1:3029061/70‑1 (MQ=255) tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc < 1:2742127/70‑1 (MQ=255) tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc < 1:2565881/70‑1 (MQ=255) catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc < 1:169395/71‑1 (MQ=255) catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc < 1:720934/71‑1 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGaa > 1:2818811/1‑44 (MQ=16) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGaa > 1:2478943/1‑44 (MQ=16) atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCa < 1:171742/71‑1 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc > 1:2837136/1‑56 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc > 1:1547707/1‑70 (MQ=255) ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTAtt < 1:3586881/65‑1 (MQ=255) tctGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGat > 1:2069477/3‑49 (MQ=255) cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:300223/43‑1 (MQ=255) aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGatat > 1:2091941/1‑44 (MQ=255) aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata > 1:1347391/1‑43 (MQ=255) aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTAGGATATGCATCCGTTTATTCACCCGCTGTCTgc > 1:184044/1‑71 (MQ=255) cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggcg < 1:1316571/71‑1 (MQ=255) caCCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa < 1:3568388/49‑1 (MQ=255) aCCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGt < 1:3340782/71‑1 (MQ=255) ccTTGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc < 1:2701348/51‑1 (MQ=255) ccTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTc > 1:1082343/1‑71 (MQ=255) ccTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc > 1:523411/1‑71 (MQ=255) ccTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc > 1:645282/1‑71 (MQ=255) cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc < 1:2702202/52‑1 (MQ=255) cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCg < 1:1147388/71‑1 (MQ=255) tGGTACGAAAAACACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc < 1:382861/52‑1 (MQ=255) ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:2218132/1‑45 (MQ=255) ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGAt > 1:539908/1‑71 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:2894652/70‑1 (MQ=255) aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg > 1:1174350/1‑71 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGttt > 1:827096/1‑38 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGttt > 1:2951607/1‑38 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCg < 1:3899757/47‑1 (MQ=255) aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt < 1:2898435/71‑3 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCt > 1:3946166/1‑71 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCt < 1:2567963/71‑1 (MQ=255) aaaGACGATATGAACACCTCGGATATGCATCCGtt < 1:3112855/35‑1 (MQ=25) aaaGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:981683/1‑37 (MQ=37) aaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTg < 1:3714343/71‑1 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc > 1:1896657/1‑60 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg > 1:2116689/1‑71 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg < 1:942542/71‑1 (MQ=255) cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa < 1:3126815/70‑1 (MQ=255) cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:404217/71‑1 (MQ=255) | TCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG > minE/833871‑834000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |