Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,942 T→C 35.7% T800T (ACT→ACC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,9420TC35.7% 50.1 / 52.6 56T800T (ACT→ACCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (16/20);  new base C (8/12);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG  >  minE/833873‑834000
                                                                     |                                                          
gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACtt                                                           >  1:1507433/1‑71 (MQ=255)
          ctctATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCa                                                 >  1:1652325/1‑71 (MQ=255)
             ttctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCAt                                                <  1:2153056/66‑1 (MQ=255)
               ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg                                             <  1:2145552/70‑1 (MQ=255)
                tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt                                           >  1:729285/1‑71 (MQ=255)
                 ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATc                                               >  1:406587/1‑66 (MQ=255)
                  cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGa                                                       >  1:2581712/1‑57 (MQ=255)
                  cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                         <  1:1145252/71‑1 (MQ=255)
                  cGATATCGTTCTGCCCACCGCCACCTGTACGAAAAAGACGATATGAACACCTCGGATATGc                                                  >  1:684424/1‑61 (MQ=255)
                   gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt                                            <  1:2051805/67‑1 (MQ=255)
                   gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt                                            <  1:3459817/67‑1 (MQ=255)
                    atatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCAt                                                >  1:3230386/3‑62 (MQ=255)
                     tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc                                      <  1:3029061/70‑1 (MQ=255)
                     tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc                                      <  1:2742127/70‑1 (MQ=255)
                     tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc                                      <  1:2565881/70‑1 (MQ=255)
                     catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc                                      <  1:169395/71‑1 (MQ=255)
                     catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc                                      <  1:720934/71‑1 (MQ=255)
                      atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCa                                     <  1:171742/71‑1 (MQ=255)
                        catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc                                                  >  1:2837136/1‑56 (MQ=255)
                        catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc                                    >  1:1547707/1‑70 (MQ=255)
                          ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTAtt                                       <  1:3586881/65‑1 (MQ=255)
                           tctGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGat                                                      >  1:2069477/3‑49 (MQ=255)
                                cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa                                                       <  1:300223/43‑1 (MQ=255)
                                  aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGatat                                                    >  1:2091941/1‑44 (MQ=255)
                                  aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata                                                     >  1:1347391/1‑43 (MQ=255)
                                  aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTAGGATATGCATCCGTTTATTCACCCGCTGTCTgc                         >  1:184044/1‑71 (MQ=255)
                                      cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggcg                     <  1:1316571/71‑1 (MQ=255)
                                       caCCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                         <  1:3568388/49‑1 (MQ=255)
                                        aCCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGt                   <  1:3340782/71‑1 (MQ=255)
                                         ccTTGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc                                      <  1:2701348/51‑1 (MQ=255)
                                         ccTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTc                  >  1:1082343/1‑71 (MQ=255)
                                         ccTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc                  >  1:523411/1‑71 (MQ=255)
                                         ccTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc                  >  1:645282/1‑71 (MQ=255)
                                          cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc                                    <  1:2702202/52‑1 (MQ=255)
                                          cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCg                 <  1:1147388/71‑1 (MQ=255)
                                           tGGTACGAAAAACACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc                                   <  1:382861/52‑1 (MQ=255)
                                            ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                         >  1:2218132/1‑45 (MQ=255)
                                            ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGAt               >  1:539908/1‑71 (MQ=255)
                                             gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt               <  1:2894652/70‑1 (MQ=255)
                                               aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg            >  1:1174350/1‑71 (MQ=255)
                                                  aaaaaGACGACATGAATACTTCGGATATGCATCCGttt                                          >  1:827096/1‑38 (MQ=255)
                                                  aaaaaGACGACATGAATACTTCGGATATGCATCCGttt                                          >  1:2951607/1‑38 (MQ=255)
                                                  aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCg                                 <  1:3899757/47‑1 (MQ=255)
                                                   aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt        <  1:2898435/71‑3 (MQ=255)
                                                   aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCt        <  1:2567963/71‑1 (MQ=255)
                                                   aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCt        >  1:3946166/1‑71 (MQ=255)
                                                    aaaGACGATATGAACACCTCGGATATGCATCCGtt                                           <  1:3112855/35‑1 (MQ=25)
                                                    aaaGACGATATGAACACCTCGGATATGCATCCGTTTa                                         >  1:981683/1‑37 (MQ=37)
                                                    aaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTg       <  1:3714343/71‑1 (MQ=255)
                                                    aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc                  >  1:1896657/1‑60 (MQ=255)
                                                    aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg       >  1:2116689/1‑71 (MQ=255)
                                                    aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg       <  1:942542/71‑1 (MQ=255)
                                                         cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa   <  1:3126815/70‑1 (MQ=255)
                                                         cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg  <  1:404217/71‑1 (MQ=255)
                                                           ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGaa   <  1:2987246/66‑1 (MQ=255)
                                                                 aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTgg      <  1:1288601/59‑1 (MQ=255)
                                                                   aCTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGa    <  1:3113469/59‑1 (MQ=255)
                                                                     |                                                          
GAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG  >  minE/833873‑834000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: