| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,029 | C→T | 19.2% | A829A (GCC→GCT) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,029 | 0 | C | T | 19.2% | 109.6 / 19.7 | 52 | A829A (GCC→GCT) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (20/22); new base T (4/6); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
TTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA > minE/833962‑834099 | ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATc > 1:1281837/1‑71 (MQ=255) tccGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATcgcca < 1:921612/70‑3 (MQ=255) tccGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATcgcca < 1:781429/70‑3 (MQ=255) tccGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATcgcca < 1:2048209/70‑3 (MQ=255) tccGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATcgcca < 1:292052/70‑3 (MQ=255) cGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATcgcca > 1:3470978/1‑67 (MQ=255) cGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATcgcg > 1:2037592/1‑68 (MQ=255) cGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATcgcg > 1:1838794/1‑68 (MQ=255) gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaag > 1:1536389/1‑70 (MQ=255) tCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATcgcg < 1:2063502/63‑1 (MQ=255) tCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATcgcg < 1:2575807/63‑1 (MQ=255) cggTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGaa < 1:1211621/71‑1 (MQ=255) gTCGATACGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAg > 1:210192/1‑70 (MQ=255) tCGATCCGGCCTGGGAAGCGAAACGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAAt < 1:1941501/60‑1 (MQ=255) tCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCg < 1:1616871/66‑1 (MQ=255) tCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtg > 1:2782389/1‑71 (MQ=255) cGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATcgcg < 1:2342961/52‑1 (MQ=255) aTCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATCGCcaaa < 1:3020136/53‑2 (MQ=255) tCCGGCCTTGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCg < 1:945227/71‑1 (MQ=255) ccGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt > 1:3054163/1‑71 (MQ=255) ccGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt > 1:1433009/1‑71 (MQ=255) cTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct > 1:3820388/1‑53 (MQ=255) ctggAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc > 1:2525992/3‑63 (MQ=255) ggAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc > 1:597351/1‑71 (MQ=255) ggAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc > 1:1586483/1‑71 (MQ=255) gAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt < 1:2591893/71‑1 (MQ=255) aaGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg < 1:1974148/71‑1 (MQ=255) aGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGaa > 1:1634938/1‑52 (MQ=255) aGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg > 1:2398472/1‑71 (MQ=255) gCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg < 1:1754002/71‑1 (MQ=255) gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg < 1:2087617/68‑1 (MQ=255) gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa < 1:1517705/71‑1 (MQ=255) gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa < 1:3754192/71‑1 (MQ=255) aaaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:3922874/71‑1 (MQ=255) aGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa > 1:2475190/1‑69 (MQ=255) gCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa < 1:3540844/70‑1 (MQ=255) aCTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAAcc < 1:880791/71‑1 (MQ=255) cTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa < 1:2596003/62‑1 (MQ=255) tGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGaaa > 1:3043177/1‑67 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc > 1:1845737/1‑70 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa > 1:2880556/1‑71 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa < 1:716772/71‑1 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa > 1:378323/1‑71 (MQ=255) gAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAcg > 1:3090338/1‑69 (MQ=255) aaaTCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc < 1:3558644/71‑1 (MQ=255) aaTCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCg < 1:2328367/71‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc > 1:335780/1‑47 (MQ=255) tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaaa > 1:2993752/1‑58 (MQ=255) aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa < 1:40990/51‑1 (MQ=255) aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaaa > 1:1087941/1‑57 (MQ=255) taGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt < 1:354189/42‑1 (MQ=255) aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCt < 1:1274669/70‑1 (MQ=255) cATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa > 1:3232936/1‑71 (MQ=255) | TTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA > minE/833962‑834099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |