| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,182 | T→C | 15.9% | G880G (GGT→GGC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,182 | 0 | T | C | 15.9% | 146.4 / 19.9 | 63 | G880G (GGT→GGC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/30); new base C (6/4); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAT > minE/834113‑834250 | tGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGt > 1:3216224/1‑70 (MQ=255) ccGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTaa < 1:1014080/70‑1 (MQ=255) cGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTaaaa > 1:3435869/1‑71 (MQ=255) gcgcAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCcaca < 1:877372/71‑1 (MQ=255) gcgcAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCcaca > 1:2600445/1‑71 (MQ=255) aGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAtt < 1:3157929/71‑1 (MQ=255) aGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAt > 1:747776/1‑70 (MQ=255) ccGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAt < 1:2032425/68‑1 (MQ=255) ccGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTAt < 1:2250446/71‑1 (MQ=255) cGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAt < 1:3408732/67‑1 (MQ=255) cTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAt < 1:13583/65‑1 (MQ=255) cTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACAt < 1:2183706/65‑1 (MQ=255) ggATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCg > 1:3828412/1‑71 (MQ=255) aTGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTa < 1:233607/63‑1 (MQ=255) aaGACTGTAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAgcgc < 1:2203861/71‑1 (MQ=255) aGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAgcgc > 1:3061287/1‑70 (MQ=255) aCTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGAt < 1:1542809/71‑1 (MQ=255) tGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTaa < 1:364169/35‑1 (MQ=255) gAAAAAAGGCGAGTGCGACCTGATCCCAGGTaaaa > 1:3710496/1‑35 (MQ=255) gAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCgcgc > 1:1986784/1‑41 (MQ=255) gAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:3722169/69‑1 (MQ=255) gAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTAt < 1:1140874/70‑1 (MQ=255) gAAAAAAGGCGAGTGCGACCAGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:3300199/69‑1 (MQ=255) aaaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:1007052/68‑1 (MQ=255) aaaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGAGCCACACAtt < 1:3055504/48‑1 (MQ=255) aaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACcgc > 1:2432850/1‑37 (MQ=255) aaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:2253779/67‑1 (MQ=255) aaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:2411981/67‑1 (MQ=255) aaaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTa < 1:1219952/67‑1 (MQ=255) aaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATg > 1:2076887/1‑49 (MQ=255) aaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTAGt < 1:549649/54‑1 (MQ=255) aaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTag > 1:1314382/1‑54 (MQ=255) ggCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGAc > 1:296099/1‑71 (MQ=255) ggCGAATGCGATCTGATTCCAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTAc < 1:834024/71‑1 (MQ=255) gCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACt < 1:3167794/71‑1 (MQ=255) cGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACtt < 1:3174812/71‑1 (MQ=255) gTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTAc > 1:92043/1‑70 (MQ=255) tGCGATCTGATTCCAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGa < 1:3664380/71‑1 (MQ=255) gACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTAc < 1:2486990/66‑1 (MQ=255) ccTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTa < 1:588364/40‑1 (MQ=255) ccTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCt > 1:953478/1‑71 (MQ=255) cTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCtt < 1:2009306/71‑1 (MQ=255) tGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCtt > 1:1517072/1‑70 (MQ=255) gATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTa > 1:2449633/1‑71 (MQ=255) attccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCtt < 1:2850084/65‑1 (MQ=255) attccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTAc > 1:1821970/4‑71 (MQ=255) aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAgc < 1:2495032/40‑1 (MQ=255) aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc < 1:600680/71‑1 (MQ=255) ttccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTAcc > 1:1029086/3‑71 (MQ=255) ttccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTAcc < 1:708956/69‑1 (MQ=255) ttccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTAcc > 1:3436047/3‑71 (MQ=255) tccAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACct > 1:198545/2‑71 (MQ=255) ccAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCgg > 1:1851382/1‑49 (MQ=255) ccAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAAc < 1:223077/61‑1 (MQ=255) cAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACctc > 1:1208483/1‑70 (MQ=255) cAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACctcg > 1:1074586/1‑70 (MQ=255) cAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACctcg > 1:2751193/1‑70 (MQ=255) aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGc < 1:1726855/48‑1 (MQ=255) aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGaa > 1:388248/1‑58 (MQ=255) aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc > 1:3209724/1‑66 (MQ=255) aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa > 1:3951602/1‑71 (MQ=255) aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa > 1:378712/1‑71 (MQ=255) ggTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAt > 1:3084154/1‑71 (MQ=255) gTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACt > 1:799961/1‑50 (MQ=255) | TGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAT > minE/834113‑834250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |