| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,656 | G→A | 9.0% | D385N (GAC→AAC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,656 | 0 | G | A | 9.0% | 162.1 / 5.4 | 56 | D385N (GAC→AAC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (25/25); new base A (3/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
AACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTT > minE/189586‑189725 | aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATg < 1:2130320/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:2772430/1‑71 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:1912756/1‑71 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg > 1:3144947/1‑68 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:30969/1‑69 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:1795071/1‑69 (MQ=255) ccACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGc > 1:577684/1‑69 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATCACGAAGAGGCCtt > 1:2614385/1‑71 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:3878029/71‑1 (MQ=255) caAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTa < 1:106517/71‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:109014/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:1440890/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:342552/1‑70 (MQ=255) aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTaa > 1:3327390/1‑71 (MQ=255) aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTaa > 1:3151622/1‑71 (MQ=255) aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGa > 1:3602599/1‑59 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAAc < 1:468205/71‑1 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg < 1:1989331/71‑1 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg < 1:2524304/71‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATACACATTAAAGGCGA‑GTATGACGAag > 1:874904/1‑57 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:949683/70‑1 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:1100066/70‑1 (MQ=255) cTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAg < 1:1421299/70‑1 (MQ=255) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCt < 1:1810438/70‑1 (MQ=9) gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat > 1:1164167/1‑71 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:1195601/45‑1 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2776494/45‑1 (MQ=255) ttATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat < 1:3050058/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:541136/1‑70 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGc < 1:1846554/70‑1 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGc > 1:1171744/1‑37 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc > 1:2429243/1‑71 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc > 1:1936249/1‑71 (MQ=255) gCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCt > 1:1620737/1‑71 (MQ=255) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc > 1:2163227/2‑71 (MQ=9) aTCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCg > 1:608139/1‑69 (MQ=255) cacaTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAg < 1:3307876/71‑1 (MQ=255) acaTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCa > 1:3013327/1‑69 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGtt < 1:723364/71‑1 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGtt < 1:2203421/71‑1 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGt > 1:14578/1‑70 (MQ=255) ttAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGc > 1:3027248/1‑37 (MQ=255) aaaGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATa < 1:3889274/49‑1 (MQ=255) aaGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatg < 1:207303/41‑1 (MQ=255) aaGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatg < 1:204087/41‑1 (MQ=255) aaGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATccc < 1:563082/71‑1 (MQ=255) aGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATacca < 1:3345295/50‑1 (MQ=255) gCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGcc < 1:2642849/58‑1 (MQ=255) cGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCTCCACCTCGCCCa < 1:3931271/59‑1 (MQ=255) cGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttg < 1:3083419/71‑1 (MQ=255) cGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttg < 1:485159/71‑1 (MQ=255) gA‑GTATGACGATGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCg < 1:3155015/54‑1 (MQ=255) gA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttgt < 1:2170908/71‑1 (MQ=255) gTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttgttg > 1:425362/1‑71 (MQ=255) tGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCtt > 1:1524118/1‑71 (MQ=255) tGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCtt > 1:822975/1‑71 (MQ=255) tGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCt > 1:818377/1‑70 (MQ=255) | AACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTT > minE/189586‑189725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |