| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,515 | A→G | 42.9% | E991E (GAA→GAG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,515 | 0 | A | G | 42.9% | 14.1 / 47.8 | 42 | E991E (GAA→GAG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base G (10/8); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTC > minE/834447‑834583 | gTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAagaaga < 1:2445255/71‑1 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACg < 1:1527250/70‑1 (MQ=255) cTGGGCTGCCCTGAGCGAATTTACCGGTCGTGCCCATACGCATCTGGCGCTGAATAAAGAAGACgag > 1:800475/1‑67 (MQ=255) cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGAt > 1:2332549/1‑64 (MQ=255) cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCtt < 1:1227682/70‑1 (MQ=255) cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCtt < 1:2870825/70‑1 (MQ=255) cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCt < 1:3683722/69‑1 (MQ=255) ccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCtt < 1:485273/69‑1 (MQ=255) tGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcg > 1:243645/1‑70 (MQ=255) aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa > 1:288234/1‑71 (MQ=255) gCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa > 1:210116/1‑70 (MQ=255) cGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGat < 1:2344688/70‑1 (MQ=255) tcaCCGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAg < 1:544803/69‑1 (MQ=255) caCCGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAgg > 1:1978356/2‑71 (MQ=255) caCCGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAgg > 1:833000/2‑71 (MQ=255) ccGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATAtt < 1:3300136/65‑1 (MQ=255) ccGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCt < 1:1319235/54‑1 (MQ=255) ccGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg < 1:1850715/71‑2 (MQ=255) ccGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg < 1:525403/71‑2 (MQ=255) cGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGatat < 1:3829395/63‑1 (MQ=255) cGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcac < 1:708986/71‑1 (MQ=255) gTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc < 1:3301163/38‑1 (MQ=255) gTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAg < 1:3711096/71‑1 (MQ=255) cGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGcc > 1:1505855/1‑71 (MQ=255) gaacATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcg > 1:2012385/4‑71 (MQ=255) aCCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg > 1:3373149/1‑70 (MQ=255) cATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCa > 1:1686105/1‑56 (MQ=255) aTACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcgc > 1:2100489/1‑47 (MQ=255) aTACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaa < 1:444828/71‑1 (MQ=255) aTACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAg > 1:1265997/1‑56 (MQ=255) cccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAt > 1:726047/3‑70 (MQ=255) gCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTa < 1:1798718/71‑1 (MQ=255) ccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgc > 1:71946/2‑62 (MQ=255) cATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAt < 1:501873/71‑1 (MQ=255) cATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAt < 1:2869360/71‑1 (MQ=255) aTCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt > 1:2402586/1‑68 (MQ=255) aTCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAtc < 1:1834838/71‑1 (MQ=255) tCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTcgc < 1:461865/40‑1 (MQ=255) tGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAgg > 1:3407723/1‑48 (MQ=255) tGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAgg > 1:3069163/1‑48 (MQ=255) gcgcTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTcc < 1:715014/69‑1 (MQ=255) cgcTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGc > 1:3406273/1‑71 (MQ=255) gAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa > 1:1145938/1‑71 (MQ=255) aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAc > 1:3447536/1‑67 (MQ=255) aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg < 1:946677/71‑1 (MQ=255) aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg > 1:2264364/1‑71 (MQ=255) aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg > 1:1272132/1‑71 (MQ=255) aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg > 1:1483700/1‑71 (MQ=255) agaggaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggt > 1:3512271/1‑71 (MQ=255) agaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtctc < 1:1289726/57‑1 (MQ=255) gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc > 1:1206579/1‑71 (MQ=255) | GTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTC > minE/834447‑834583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |