Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,548 A→G 34.5% A1002A (GCA→GCG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,5480AG34.5% 55.9 / 51.0 58A1002A (GCA→GCGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (21/17);  new base G (12/8);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01

CGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCG  >  minE/834479‑834618
                                                                     |                                                                      
cGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcac                                                                       <  1:708986/71‑1 (MQ=255)
  gTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAg                                                                     <  1:3711096/71‑1 (MQ=255)
    cGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGcc                                                                   >  1:1505855/1‑71 (MQ=255)
       gaacATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcg                                                                >  1:2012385/4‑71 (MQ=255)
        aCCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                                >  1:3373149/1‑70 (MQ=255)
           aTACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaa                                                            <  1:444828/71‑1 (MQ=255)
              cccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAt                                                          >  1:726047/3‑70 (MQ=255)
               gCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTa                                                        <  1:1798718/71‑1 (MQ=255)
               ccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgc                                                                 >  1:71946/2‑62 (MQ=255)
                cATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAt                                                       <  1:501873/71‑1 (MQ=255)
                cATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAt                                                       <  1:2869360/71‑1 (MQ=255)
                 aTCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt                                                         >  1:2402586/1‑68 (MQ=255)
                 aTCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAtc                                                      <  1:1834838/71‑1 (MQ=255)
                      gcgcTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTcc                                                   <  1:715014/69‑1 (MQ=255)
                       cgcTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGc                                                >  1:3406273/1‑71 (MQ=255)
                           gAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa                                            >  1:1145938/1‑71 (MQ=255)
                                aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAc                                           >  1:3447536/1‑67 (MQ=255)
                                aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg                                       >  1:2264364/1‑71 (MQ=255)
                                aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg                                       <  1:946677/71‑1 (MQ=255)
                                aagaggACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctgg                                       >  1:1272132/1‑71 (MQ=255)
                                aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg                                       >  1:1483700/1‑71 (MQ=255)
                                 agaggaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggt                                      >  1:3512271/1‑71 (MQ=255)
                                 agaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtctc                                                    <  1:1289726/57‑1 (MQ=255)
                                  gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc                                     >  1:1206579/1‑71 (MQ=255)
                                     gaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctgg                                  >  1:3154823/1‑71 (MQ=255)
                                     gaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctgg                                  >  1:92992/1‑71 (MQ=255)
                                       cGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCtggtccggtc                                >  1:3442243/1‑71 (MQ=255)
                                        gagaAGATTCGCTTTCGCGATATTCAGGCGCAGCcgc                                                                 <  1:584969/37‑1 (MQ=25)
                                        gagaAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCtggtccggtc                                <  1:1052559/70‑1 (MQ=255)
                                        gagaAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCa                                                              >  1:719255/1‑40 (MQ=255)
                                        gagaAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCa                                                              >  1:795493/1‑40 (MQ=255)
                                          gaagaTTCGCTTTCGCGATATTCAGGCGCAgcggcgt                                                               >  1:2152428/1‑36 (MQ=16)
                                          gaagaTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGgtctggtctg                              >  1:2688955/1‑70 (MQ=255)
                                            agaTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGa                            <  1:2507330/70‑1 (MQ=255)
                                             gaTTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGa                            >  1:2016113/1‑69 (MQ=255)
                                             gaTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGgtctgtctgg                             <  1:1862095/67‑1 (MQ=255)
                                               tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGaa                           >  1:272111/1‑68 (MQ=255)
                                               tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGAt                        >  1:2720303/1‑71 (MQ=255)
                                               tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGAt                        >  1:2658065/1‑71 (MQ=255)
                                                tcGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt                                                         <  1:958024/36‑1 (MQ=25)
                                                 cGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGa                      <  1:579043/71‑1 (MQ=255)
                                                  gCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAt                                                       >  1:1614774/1‑37 (MQ=255)
                                                  gCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGaa                     <  1:597578/71‑1 (MQ=255)
                                                   cTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAac                    <  1:17509/71‑1 (MQ=255)
                                                    ttCCGCGATATTCAGGCACAGCCGCGCAAAATTAt                                                       <  1:3553451/35‑1 (MQ=255)
                                                    ttCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg                                       >  1:3934788/1‑51 (MQ=255)
                                                     tCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGaa                     >  1:3157990/1‑68 (MQ=255)
                                                     tCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                  >  1:162863/1‑71 (MQ=255)
                                                      ccGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                  >  1:1261589/1‑70 (MQ=255)
                                                      ccGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                  <  1:250499/70‑1 (MQ=255)
                                                           atatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt            <  1:3872957/71‑1 (MQ=255)
                                                            tatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCtt           <  1:1375015/71‑1 (MQ=255)
                                                              ttCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCtggtccggtc                                <  1:366804/48‑1 (MQ=255)
                                                              ttCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctg                                   <  1:283827/45‑1 (MQ=255)
                                                                cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa          >  1:3752800/1‑68 (MQ=255)
                                                                cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa          >  1:653439/1‑68 (MQ=255)
                                                                cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa        <  1:1228092/70‑1 (MQ=255)
                                                                 cggCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTAc         <  1:550323/67‑1 (MQ=255)
                                                                 aGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAAc      >  1:1431713/1‑71 (MQ=255)
                                                                     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc   <  1:710114/70‑1 (MQ=255)
                                                                     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg  >  1:526679/1‑71 (MQ=255)
                                                                     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg  >  1:3223269/1‑71 (MQ=255)
                                                                     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg  >  1:3656926/1‑71 (MQ=255)
                                                                     |                                                                      
CGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCG  >  minE/834479‑834618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: