Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,599 A→T 23.9% E1019D (GAA→GAT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,5990AT23.9% 96.0 / 26.5 54E1019D (GAA→GATnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (24/17);  new base T (4/9);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGT  >  minE/834529‑834668
                                                                      |                                                                     
gCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGaa                                                                       <  1:597578/71‑1 (MQ=255)
 cTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAac                                                                      <  1:17509/71‑1 (MQ=255)
   tCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGaa                                                                       >  1:3157990/1‑68 (MQ=255)
   tCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                                                                    >  1:162863/1‑71 (MQ=255)
    ccGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                                                                    <  1:250499/70‑1 (MQ=255)
    ccGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAacac                                                                    >  1:1261589/1‑70 (MQ=255)
         atatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt                                                              <  1:3872957/71‑1 (MQ=255)
          tatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCtt                                                             <  1:1375015/71‑1 (MQ=255)
              cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa                                                            >  1:3752800/1‑68 (MQ=255)
              cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa                                                            >  1:653439/1‑68 (MQ=255)
              cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa                                                          <  1:1228092/70‑1 (MQ=255)
               cggCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTAc                                                           <  1:550323/67‑1 (MQ=255)
               aGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAAc                                                        >  1:1431713/1‑71 (MQ=255)
                 gcgcaGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACg                                                       <  1:3136073/67‑1 (MQ=255)
                   acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc                                                     <  1:710114/70‑1 (MQ=255)
                   acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                    >  1:3223269/1‑71 (MQ=255)
                   acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                    >  1:526679/1‑71 (MQ=255)
                   acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                    >  1:3656926/1‑71 (MQ=255)
                           gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACAcc                                            <  1:1113689/71‑1 (MQ=255)
                            taaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCggga                                                  <  1:3233394/63‑2 (MQ=255)
                            cAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                             >  1:2292212/1‑69 (MQ=255)
                             aaaaTTATCTCCAGCCCGACCTGGTCTGGTCTGGATGATGAACACGTTTCTTACAACGCCGGTTACACCaa                                          >  1:1759730/1‑71 (MQ=255)
                             aaaaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCtt                                                             >  1:3949103/1‑52 (MQ=255)
                              aaaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAAc                                         >  1:3283398/1‑71 (MQ=255)
                              aaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAAc                                         >  1:3884871/1‑71 (MQ=255)
                               aaTTATCTCCAGCCCGCCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAAc                                         >  1:2240582/1‑70 (MQ=255)
                                aTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa                                                >  1:3101921/1‑62 (MQ=255)
                                aTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGt                                       <  1:2394697/71‑1 (MQ=255)
                                aTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGt                                       <  1:2390005/71‑1 (MQ=255)
                                 ttATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATa                                                <  1:2956570/61‑1 (MQ=255)
                                 ttATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATa                                                <  1:3339452/61‑1 (MQ=255)
                                   aTCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAAc                                                        >  1:1464103/1‑51 (MQ=255)
                                    tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCgg                                                   >  1:49419/1‑55 (MQ=255)
                                        cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGa                                 <  1:3493738/69‑1 (MQ=255)
                                        cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                               >  1:1885374/1‑71 (MQ=255)
                                         aGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt                                                              >  1:898653/1‑39 (MQ=255)
                                            ccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGAt                           >  1:2931444/1‑71 (MQ=255)
                                             cGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATc                          >  1:2420899/1‑71 (MQ=255)
                                                ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                       <  1:997730/71‑1 (MQ=255)
                                                 ctggtccggtCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCat                                   <  1:2195101/58‑2 (MQ=255)
                                                    gtctggtctgGAAGATGAACACTTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                       <  1:2066342/67‑1 (MQ=255)
                                                    gtctggtctgGAAGATGAACACGTTTCTTACAACGc                                                      <  1:2911476/36‑1 (MQ=255)
                                                    gtccggtctgGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTgg                    <  1:3774248/70‑1 (MQ=255)
                                                        ggtctggAAGATTAACACGTTTCTTACAACGCCGGTTa                                                <  1:3135240/38‑1 (MQ=255)
                                                         gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg              >  1:578677/1‑71 (MQ=255)
                                                         gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg              >  1:1977973/1‑71 (MQ=255)
                                                          tctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg              <  1:3428785/70‑1 (MQ=255)
                                                              gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct          <  1:1248991/70‑1 (MQ=255)
                                                              gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct          <  1:887036/70‑1 (MQ=255)
                                                              gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct          <  1:1625502/70‑1 (MQ=255)
                                                                aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg       >  1:3331147/1‑71 (MQ=255)
                                                                aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg       >  1:1381597/1‑71 (MQ=255)
                                                                aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg       >  1:587615/1‑71 (MQ=255)
                                                                aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg       >  1:1127115/1‑71 (MQ=255)
                                                                aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg       >  1:3472485/1‑71 (MQ=255)
                                                                 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg      <  1:893974/71‑1 (MQ=255)
                                                                 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg      <  1:2667360/71‑1 (MQ=255)
                                                                  aTGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                       <  1:897873/53‑1 (MQ=255)
                                                                   tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc    >  1:2139387/1‑71 (MQ=255)
                                                                    gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg                     >  1:667060/1‑53 (MQ=255)
                                                                     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt  <  1:1873440/71‑1 (MQ=255)
                                                                     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt  <  1:552425/71‑1 (MQ=255)
                                                                      acacGTTTCTTACAACGCCGGTTACACCAACGTTCa                                    >  1:3526942/1‑36 (MQ=255)
                                                                      acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                >  1:1072750/1‑40 (MQ=255)
                                                                      acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                  >  1:1314246/1‑54 (MQ=255)
                                                                      acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                  >  1:2481734/1‑54 (MQ=255)
                                                                      |                                                                     
GCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGT  >  minE/834529‑834668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: