Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,611 C→T 26.8% Y1023Y (TAC→TAT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6110CT26.8% 110.4 / 40.1 67Y1023Y (TAC→TATnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (31/18);  new base T (7/11);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 9.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTA  >  minE/834543‑834679
                                                                    |                                                                    
cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa                                                                     <  1:1228092/70‑1 (MQ=255)
 cggCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTAc                                                                      <  1:550323/67‑1 (MQ=255)
 aGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAAc                                                                   >  1:1431713/1‑71 (MQ=255)
   gcgcaGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACg                                                                  <  1:3136073/67‑1 (MQ=255)
     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc                                                                <  1:710114/70‑1 (MQ=255)
     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                               >  1:526679/1‑71 (MQ=255)
     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                               >  1:3223269/1‑71 (MQ=255)
     acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                               >  1:3656926/1‑71 (MQ=255)
             gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACAcc                                                       <  1:1113689/71‑1 (MQ=255)
              taaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCggga                                                             <  1:3233394/63‑2 (MQ=255)
              cAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                                        >  1:2292212/1‑69 (MQ=255)
               aaaaTTATCTCCAGCCCGACCTGGTCTGGTCTGGATGATGAACACGTTTCTTACAACGCCGGTTACACCaa                                                     >  1:1759730/1‑71 (MQ=255)
                aaaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAAc                                                    >  1:3283398/1‑71 (MQ=255)
                aaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAAc                                                    >  1:3884871/1‑71 (MQ=255)
                 aaTTATCTCCAGCCCGCCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAAc                                                    >  1:2240582/1‑70 (MQ=255)
                  aTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa                                                           >  1:3101921/1‑62 (MQ=255)
                  aTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGt                                                  <  1:2394697/71‑1 (MQ=255)
                  aTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGt                                                  <  1:2390005/71‑1 (MQ=255)
                   ttATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATa                                                           <  1:3339452/61‑1 (MQ=255)
                   ttATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATa                                                           <  1:2956570/61‑1 (MQ=255)
                     aTCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAAc                                                                   >  1:1464103/1‑51 (MQ=255)
                      tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCgg                                                              >  1:49419/1‑55 (MQ=255)
                          cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGa                                            <  1:3493738/69‑1 (MQ=255)
                          cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                                          >  1:1885374/1‑71 (MQ=255)
                              ccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGAt                                      >  1:2931444/1‑71 (MQ=255)
                               cGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATc                                     >  1:2420899/1‑71 (MQ=255)
                                  ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                  <  1:997730/71‑1 (MQ=255)
                                   ctggtccggtCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCat                                              <  1:2195101/58‑2 (MQ=255)
                                      gtctggtctgGAAGATGAACACTTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                  <  1:2066342/67‑1 (MQ=255)
                                      gtctggtctgGAAGATGAACACGTTTCTTACAACGc                                                                 <  1:2911476/36‑1 (MQ=255)
                                      gtccggtctgGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTgg                               <  1:3774248/70‑1 (MQ=255)
                                          ggtctggAAGATTAACACGTTTCTTACAACGCCGGTTa                                                           <  1:3135240/38‑1 (MQ=255)
                                           gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg                         >  1:1977973/1‑71 (MQ=255)
                                           gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg                         >  1:578677/1‑71 (MQ=255)
                                            tctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg                         <  1:3428785/70‑1 (MQ=255)
                                                gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct                     <  1:1248991/70‑1 (MQ=255)
                                                gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct                     <  1:887036/70‑1 (MQ=255)
                                                gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct                     <  1:1625502/70‑1 (MQ=255)
                                                  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                  >  1:587615/1‑71 (MQ=255)
                                                  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                  >  1:1381597/1‑71 (MQ=255)
                                                  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                  >  1:3472485/1‑71 (MQ=255)
                                                  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                  >  1:3331147/1‑71 (MQ=255)
                                                  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                  >  1:1127115/1‑71 (MQ=255)
                                                   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                 <  1:893974/71‑1 (MQ=255)
                                                   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                 <  1:2667360/71‑1 (MQ=255)
                                                    aTGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                  <  1:897873/53‑1 (MQ=255)
                                                     tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc               >  1:2139387/1‑71 (MQ=255)
                                                      gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg                                >  1:667060/1‑53 (MQ=255)
                                                       aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt             <  1:1873440/71‑1 (MQ=255)
                                                       aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt             <  1:552425/71‑1 (MQ=255)
                                                        tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGctgt                     <  1:1475867/61‑3 (MQ=255)
                                                        tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc             >  1:1666397/2‑69 (MQ=255)
                                                        acacGTTTCTTACAACGCCGGTTACACCAACGTTCa                                               >  1:3526942/1‑36 (MQ=255)
                                                        acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                           >  1:1072750/1‑40 (MQ=255)
                                                        acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                             >  1:1314246/1‑54 (MQ=255)
                                                        acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                             >  1:2481734/1‑54 (MQ=255)
                                                         cacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctc                      >  1:189805/1‑60 (MQ=255)
                                                         cacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGctgtc                    >  1:826260/1‑62 (MQ=255)
                                                          acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa                           >  1:1628715/1‑54 (MQ=255)
                                                          acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa                           >  1:2798653/1‑54 (MQ=255)
                                                          acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa                           >  1:1998141/1‑54 (MQ=255)
                                                          acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc             <  1:159881/68‑2 (MQ=255)
                                                          acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg          >  1:1962048/1‑71 (MQ=255)
                                                           cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg                                        <  1:1888435/40‑1 (MQ=255)
                                                               tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa           >  1:3565001/1‑65 (MQ=255)
                                                               tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa           >  1:3464236/1‑65 (MQ=255)
                                                               tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt     >  1:1259746/1‑71 (MQ=255)
                                                                   aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa  <  1:2709250/70‑1 (MQ=255)
                                                                    |                                                                    
CAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTA  >  minE/834543‑834679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: