| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,653 | T→C | 18.5% | R1037R (CGT→CGC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,653 | 0 | T | C | 18.5% | 175.3 / 32.6 | 81 | R1037R (CGT→CGC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/31); new base C (6/9); total (41/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTG > minE/834586‑834722 | gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg > 1:578677/1‑71 (MQ=255)gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg > 1:1977973/1‑71 (MQ=255) tctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg < 1:3428785/70‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct < 1:887036/70‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct < 1:1248991/70‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct < 1:1625502/70‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:1127115/1‑71 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:3331147/1‑71 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:587615/1‑71 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:1381597/1‑71 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:3472485/1‑71 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:893974/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:2667360/71‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc > 1:2139387/1‑71 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt < 1:552425/71‑1 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt < 1:1873440/71‑1 (MQ=255) tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGctgt < 1:1475867/61‑3 (MQ=255) tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc > 1:1666397/2‑69 (MQ=255) cacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctc > 1:189805/1‑60 (MQ=255) cacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGctgtc > 1:826260/1‑62 (MQ=255) acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa > 1:1628715/1‑54 (MQ=255) acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa > 1:2798653/1‑54 (MQ=255) acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTa > 1:1998141/1‑54 (MQ=255) acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc < 1:159881/68‑2 (MQ=255) acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg > 1:1962048/1‑71 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa > 1:3464236/1‑65 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa > 1:3565001/1‑65 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1259746/1‑71 (MQ=255) tataaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCa < 1:2323948/59‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa < 1:2709250/70‑1 (MQ=255) taaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTa < 1:3553339/68‑1 (MQ=255) aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt > 1:2042326/1‑69 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCa < 1:965805/70‑1 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg > 1:807224/1‑71 (MQ=255) cgcgGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc < 1:281607/55‑2 (MQ=255) cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa < 1:2240643/61‑1 (MQ=255) cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg < 1:2041313/71‑1 (MQ=255) cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt > 1:1650743/1‑70 (MQ=255) ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt < 1:3104310/63‑1 (MQ=255) ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt < 1:2732990/63‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctc < 1:2077042/41‑1 (MQ=255) ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc > 1:1316801/1‑70 (MQ=255) tACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatcc < 1:3462352/71‑4 (MQ=255) tACACCAACGTTCACGAGCTTATCCCATGGCGTACGCTCTCTGGTCGTCa > 1:655461/1‑50 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:3221082/1‑56 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:2049253/1‑56 (MQ=255) acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa < 1:3838051/70‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc < 1:7733/67‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg < 1:3906863/71‑1 (MQ=255) cAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:2888050/1‑36 (MQ=255) cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt > 1:1363833/1‑40 (MQ=255) cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa > 1:1683362/1‑71 (MQ=255) cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa > 1:107192/1‑71 (MQ=255) gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGc > 1:2720589/1‑70 (MQ=255) gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg > 1:3904524/1‑71 (MQ=255) tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt > 1:2369895/1‑70 (MQ=255) tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt > 1:1241453/1‑70 (MQ=255) cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg > 1:2554934/1‑60 (MQ=255) cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt < 1:2117088/71‑1 (MQ=255) cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt < 1:269066/71‑1 (MQ=255) cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt < 1:202207/71‑1 (MQ=255) gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt < 1:205397/71‑1 (MQ=255) aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa < 1:2546258/37‑1 (MQ=255) cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt < 1:906536/68‑1 (MQ=255) gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg < 1:519447/68‑1 (MQ=255) aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg < 1:2906606/53‑1 (MQ=255) aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt < 1:1306503/69‑1 (MQ=255) aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa < 1:3213661/71‑1 (MQ=255) tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa > 1:1478975/1‑54 (MQ=255) tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa > 1:1831850/1‑54 (MQ=255) tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa > 1:3933976/1‑70 (MQ=255) ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg > 1:3103255/1‑71 (MQ=255) cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc > 1:3604711/1‑71 (MQ=255) aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc > 1:1294340/1‑71 (MQ=255) tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc < 1:631000/43‑1 (MQ=255) tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc < 1:3759842/43‑1 (MQ=255) tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct > 1:649083/1‑71 (MQ=255) tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct < 1:3139982/71‑1 (MQ=255) gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa < 1:598416/63‑1 (MQ=255) gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa < 1:3624194/63‑1 (MQ=255) gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc < 1:1219791/71‑1 (MQ=255) cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg < 1:2218364/69‑1 (MQ=255) gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg < 1:2237273/71‑1 (MQ=255) | GTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTG > minE/834586‑834722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |