Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,662 T→G 20.8% S1040S (TCT→TCG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6620TG20.8% 119.7 / 44.8 77S1040S (TCT→TCGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base G (6/10);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834593‑834732
                                                                     |                                                                      
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       >  1:3472485/1‑71 (MQ=255)
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       >  1:1127115/1‑71 (MQ=255)
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       >  1:1381597/1‑71 (MQ=255)
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       >  1:587615/1‑71 (MQ=255)
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       >  1:3331147/1‑71 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:2667360/71‑1 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:893974/71‑1 (MQ=255)
   tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc                                                                    >  1:2139387/1‑71 (MQ=255)
     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  <  1:1873440/71‑1 (MQ=255)
     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  <  1:552425/71‑1 (MQ=255)
      tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  >  1:1666397/2‑69 (MQ=255)
        acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  <  1:159881/68‑2 (MQ=255)
        acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg                                                               >  1:1962048/1‑71 (MQ=255)
             tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:3464236/1‑65 (MQ=255)
             tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:3565001/1‑65 (MQ=255)
             tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:1259746/1‑71 (MQ=255)
                tataaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCa                                                                <  1:2323948/59‑1 (MQ=255)
                 aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                       <  1:2709250/70‑1 (MQ=255)
                  taaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTa                                                       <  1:3553339/68‑1 (MQ=255)
                   aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      >  1:2042326/1‑69 (MQ=255)
                    aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCa                                                    <  1:965805/70‑1 (MQ=255)
                    aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                   >  1:807224/1‑71 (MQ=255)
                     cgcgGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  <  1:281607/55‑2 (MQ=255)
                     cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            <  1:2240643/61‑1 (MQ=255)
                     cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                  <  1:2041313/71‑1 (MQ=255)
                        cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                                >  1:1650743/1‑70 (MQ=255)
                         ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      <  1:3104310/63‑1 (MQ=255)
                         ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      <  1:2732990/63‑1 (MQ=255)
                           ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             >  1:1316801/1‑70 (MQ=255)
                            tACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatcc                                           <  1:3462352/71‑4 (MQ=255)
                            tACACCAACGTTCACGAGCTTATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:655461/1‑50 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:3221082/1‑56 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:2049253/1‑56 (MQ=255)
                             acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                           <  1:3838051/70‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:7733/67‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3906863/71‑1 (MQ=255)
                                 cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt                                                                     >  1:1363833/1‑40 (MQ=255)
                                 cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:107192/1‑71 (MQ=255)
                                 cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1683362/1‑71 (MQ=255)
                                     gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGc                                   >  1:2720589/1‑70 (MQ=255)
                                     gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg                                  >  1:3904524/1‑71 (MQ=255)
                                       tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:2369895/1‑70 (MQ=255)
                                       tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:1241453/1‑70 (MQ=255)
                                        cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                          >  1:2554934/1‑60 (MQ=255)
                                          cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:202207/71‑1 (MQ=255)
                                          cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:2117088/71‑1 (MQ=255)
                                          cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:269066/71‑1 (MQ=255)
                                           gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            <  1:205397/71‑1 (MQ=255)
                                            aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa                                                             <  1:2546258/37‑1 (MQ=255)
                                              cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            <  1:906536/68‑1 (MQ=255)
                                                gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                          <  1:519447/68‑1 (MQ=255)
                                                 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:2906606/53‑1 (MQ=255)
                                                 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                        <  1:1306503/69‑1 (MQ=255)
                                                 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      <  1:3213661/71‑1 (MQ=255)
                                                  tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1478975/1‑54 (MQ=255)
                                                  tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1831850/1‑54 (MQ=255)
                                                  tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      >  1:3933976/1‑70 (MQ=255)
                                                    ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                   >  1:3103255/1‑71 (MQ=255)
                                                     cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                  >  1:3604711/1‑71 (MQ=255)
                                                      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc                 >  1:1294340/1‑71 (MQ=255)
                                                       tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:3759842/43‑1 (MQ=255)
                                                       tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:631000/43‑1 (MQ=255)
                                                       tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                >  1:649083/1‑71 (MQ=255)
                                                       tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                <  1:3139982/71‑1 (MQ=255)
                                                         gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                      <  1:3624194/63‑1 (MQ=255)
                                                         gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                      <  1:598416/63‑1 (MQ=255)
                                                         gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc              <  1:1219791/71‑1 (MQ=255)
                                                          cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg               <  1:2218364/69‑1 (MQ=255)
                                                           gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg            <  1:2237273/71‑1 (MQ=255)
                                                             aCGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggc          >  1:2513776/1‑70 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                        >  1:1391373/1‑56 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         <  1:1766230/71‑1 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt          <  1:1069052/70‑1 (MQ=255)
                                                               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct         >  1:1339550/1‑68 (MQ=255)
                                                               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct         >  1:921693/1‑68 (MQ=255)
                                                               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt        <  1:1594662/71‑1 (MQ=255)
                                                               gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:3119623/1‑70 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:602307/1‑50 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:1459784/1‑50 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:1865375/1‑50 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       <  1:211357/71‑1 (MQ=255)
                                                                    ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                    >  1:2364691/1‑54 (MQ=255)
                                                                    ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt   >  1:372095/1‑71 (MQ=255)
                                                                     tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc  >  1:1663510/1‑71 (MQ=255)
                                                                     |                                                                      
AGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834593‑834732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: